Restriction map for
pF1KB5815
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BlnI BpmI BpuAI BsaBI BsaI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SbfI
ScaI SfcI SfuI SnaBI SpeI
Sse8387I SspBI SspI SwaI XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 130 CGCG CG^CG
AcsI 585 GAATTT [GA]^AATT[CT]
AocI 1144 CCTGAGG CC^T[ACGT]AGG
ApaI 847 GGGCCC GGGCC^C
ApoI 585 GAATTT [GA]^AATT[CT]
AvaI 164 CCCGGG C^[CT]CG[GA]G
BbeI 446 GGCGCC GGCGC^C
BglI 1331 GCCTGACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1280 AGATCT A^GATCT
BlpI 1029 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1029 GCTGAGC GC^T[ACGT]AGC
BsiEI 132 CGACCG CG[GA][CT]^CG
BsmAI 619 GAGAC GTCTC(1/5)
BsmI 1020 GAATGC GAATGC(1/-1)
BsoBI 164 CCCGGG C^[CT]CG[GA]G
BspHI 1127 TCATGA T^CATGA
BsrDI 948 CATTGC GCAATG(2/0)
BssSI 244 CACGAG CACGAG(-5/-1)
BstUI 130 CGCG CG^CG
Bsu36I 1144 CCTGAGG CC^T[ACGT]AGG
CelII 1029 GCTGAGC GC^T[ACGT]AGC
DrdI 925 GACATCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1101 CGGCCA [CT]^GGCC[GA]
EarI 359 CTCTTC CTCTTC(1/4)
Eco81I 1144 CCTGAGG CC^T[ACGT]AGG
EcoNI 867 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 896 CCAAGG C^C[AT][AT]GG
KasI 446 GGCGCC G^GCGCC
Ksp632I 359 CTCTTC CTCTTC(1/4)
MseI 572 TTAA T^TAA
MvnI 130 CGCG CG^CG
NarI 446 GGCGCC GG^CGCC
PflMI 627 CCAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1115 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1110 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 970 CAGCTG CAG^CTG
RcaI 1127 TCATGA T^CATGA
SacI 1080 GAGCTC GAGCT^C
SapI 358 GCTCTTC GCTCTTC(1/4)
SfiI 1330 GGCCTGACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 446 GGCGCC GGC^GCC
SgrAI 404 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 164 CCCGGG CCC^GGG
SmlI 853 CTCAAG C^T[CT][GA]AG
SphI 965 GCATGC GCATG^C
StyI 896 CCAAGG C^C[AT][AT]GG
Tru9I 572 TTAA T^TAA
Tsp509I 586 AATT ^AATT
Van91I 627 CCAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 696 CCAGCCGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 164 CCCGGG C^CCGGG
XmaI 164 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AlwNI 350 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
1327 CAGGGCCTG
AspI 817 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1252 GACGGCGTC
BsaAI 454 TACGTG [CT]AC^GT[GA]
919 TACGTG
BstXI 537 CCAGGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1352 CCAACAGAGTGG
DsaI 413 CCGTGG C^C[GA][CT]GG
884 CCACGG
MspA1I 151 CAGCGG C[AC]G^C[GT]G
970 CAGCTG
NspI 710 GCATGT [GA]CATG^[CT]
965 GCATGC
PflFI 817 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1252 GACGGCGTC
PleI 766 GACTC GAGTC(4/5)
1387 GACTC
SexAI 818 ACCAGGT A^CC[AT]GGT
866 ACCTGGT
StuI 682 AGGCCT AGG^CCT
857 AGGCCT
TfiI 228 GATTC G^A[AT]TC
345 GATTC
Tth111I 817 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1252 GACGGCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaII BanI BanII BbvI
BcnI BmyI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII