Restriction map for
pF1KB5837
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AlwNI Aor51HI
AscI AseI AsnI Asp700I AviII
AvrII BalI BbeI BbsI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BseAI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraII DrdI EagI EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 148 GGTACC G^GTACC
AcsI 190 AAATTT [GA]^AATT[CT]
AhdI 301 GACCCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 74 GTGCAC G^TGCAC
AocI 223 CCTGAGG CC^T[ACGT]AGG
ApaI 612 GGGCCC GGGCC^C
ApaLI 74 GTGCAC G^TGCAC
ApoI 190 AAATTT [GA]^AATT[CT]
Asp718I 148 GGTACC G^GTACC
AspEI 301 GACCCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 74 GTGCAC G[AT]GC[AT]^C
AspI 422 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 121 CCCGAG C^[CT]CG[GA]G
BamHI 26 GGATCC G^GATCC
BanI 148 GGTACC G^G[CT][GA]CC
BbrPI 77 CACGTG CAC^GTG
BglI 703 GCCCCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 641 CTGGAG CTGGAG(16/14)
BsaAI 77 CACGTG [CT]AC^GT[GA]
BsaI 631 GAGACC GGTCTC(1/5)
BsaWI 429 TCCGGT [AT]^CCGG[AT]
BseRI 644 GAGGAG GAGGAG(10/8)
BsiHKAI 74 GTGCAC G[AT]GC[AT]^C
BsmAI 631 GAGAC GTCTC(1/5)
BsmFI 13 GTCCC GGGAC(10/14)
BsoBI 121 CCCGAG C^[CT]CG[GA]G
BspMI 385 ACCTGC ACCTGC(4/8)
BsrI 242 CCAGT ACTGG(1/-1)
BssSI 628 CACGAG CACGAG(-5/-1)
BstXI 452 CCACCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 26 GGATCC [GA]^GATC[CT]
Bsu36I 223 CCTGAGG CC^T[ACGT]AGG
DpnII 27 GATC ^GATC
DraIII 77 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 329 TGGCCG [CT]^GGCC[GA]
Eam1105I 301 GACCCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 115 CTCTTC CTCTTC(1/4)
Eco81I 223 CCTGAGG CC^T[ACGT]AGG
EcoNI 544 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 273 GGCGCT [GA]GCGC^[CT]
KpnI 148 GGTACC GGTAC^C
Ksp632I 115 CTCTTC CTCTTC(1/4)
KspI 22 CCGCGG CCGC^GG
MboI 27 GATC ^GATC
MflI 26 GGATCC [GA]^GATC[CT]
NcoI 608 CCATGG C^CATGG
NdeII 27 GATC ^GATC
NspI 465 GCATGC [GA]CATG^[CT]
PflFI 422 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 603 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 77 CACGTG CAC^GTG
PmlI 77 CACGTG CAC^GTG
PvuII 262 CAGCTG CAG^CTG
SacII 22 CCGCGG CCGC^GG
Sau3AI 27 GATC ^GATC
SphI 465 GCATGC GCATG^C
Tsp509I 191 AATT ^AATT
Tth111I 422 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 603 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 26 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 149 GTAC GT^AC
582 GTAC
AlwI 27 GATCC GGATC(4/5)
26 GGATC
AvaII 12 GGTCC G^G[AT]CC
511 GGACC
BanII 267 GGGCTC G[GA]GC[CT]^C
612 GGGCCC
BcnI 70 CCGGG CC^[GC]GG
687 CCCGG
EcoT14I 326 CCTTGG C^C[AT][AT]GG
608 CCATGG
FauI 16 CCCGC CCCGC(4/6)
664 CCCGC
HinfI 107 GACTC G^A[ACGT]TC
124 GAGTC
MaeIII 364 GTCAC ^GT[ACGT]AC
436 GTCAC
MboII 186 GAAGA GAAGA(8/7)
116 TCTTC
MspA1I 22 CCGCGG C[AC]G^C[GT]G
262 CAGCTG
NciI 70 CCGGG CC^[GC]GG
687 CCCGG
PleI 107 GACTC GAGTC(4/5)
124 GAGTC
RsaI 149 GTAC GT^AC
582 GTAC
SfaNI 555 GATGC GCATC(5/9)
700 GATGC
StyI 326 CCTTGG C^C[AT][AT]GG
608 CCATGG
Tsp45I 364 GTCAC ^GT[GC]AC
436 GTCAC
TspRI 243 CAGTG CA[GC]TG[ACGT][ACGT]^
456 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccII AciI AluI BbvCI BbvI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrFI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DsaI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeII MnlI MslI MspI
MvaI MvnI MwoI NlaIII NlaIV
Sau96I ScrFI TseI