Restriction map for
pF1KB5852
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
Acc65I AccII AccIII AciI AclI
AflII AflIII AhdI AlwNI AocI
Aor51HI ApaI AscI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaI
BseAI BseRI BsgI BsiEI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI KasI
KpnI KspI MfeI MluI MroI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
TaqI Tth111I TthHB8I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 85 GACGTC GACGT^C
AccI 845 GTCTAC GT^[AC][GT]AC
AcyI 85 GACGTC G[GA]^CG[CT]C
AgeI 323 ACCGGT A^CCGGT
Alw44I 595 GTGCAC G^TGCAC
ApaLI 595 GTGCAC G^TGCAC
AseI 249 ATTAAT AT^TAAT
AsnI 249 ATTAAT AT^TAAT
AvaII 1101 GGACC G^G[AT]CC
BanI 313 GGCACC G^G[CT][GA]CC
BpmI 427 CTGGAG CTGGAG(16/14)
BsaBI 808 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 85 GACGTC G[GA]^CG[CT]C
BsaWI 323 ACCGGT [AT]^CCGG[AT]
BsiWI 130 CGTACG C^GTACG
BsmAI 542 GAGAC GTCTC(1/5)
BsmFI 590 GGGAC GGGAC(10/14)
Bsp1407I 68 TGTACA T^GTACA
BsrFI 323 ACCGGT [GA]^CCGG[CT]
BsrGI 68 TGTACA T^GTACA
BstXI 56 CCAACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 232 GCGC GCG^C
Cfr10I 323 ACCGGT [GA]^CCGG[CT]
DraII 1100 AGGACCT [GA]G^G[ACGT]CC[CT]
EarI 537 GAAGAG CTCTTC(1/4)
EcoO109I 1100 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 913 CCATGG C^C[AT][AT]GG
HaeII 231 GGCGCT [GA]GCGC^[CT]
HgaI 164 GCGTC GACGC(5/10)
HhaI 232 GCGC GCG^C
Hin1I 85 GACGTC G[GA]^CG[CT]C
HinP1I 232 GCGC G^CGC
HincII 1383 GTTAAC GT[CT]^[GA]AC
HindII 1383 GTTAAC GT[CT]^[GA]AC
HindIII 141 AAGCTT A^AGCTT
HpaI 1383 GTTAAC GTT^AAC
HphI 52 TCACC GGTGA(8/7)
Ksp632I 537 GAAGAG CTCTTC(1/4)
MamI 808 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 913 CCATGG C^CATGG
NspI 59 ACATGC [GA]CATG^[CT]
PflMI 932 CCACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 323 ACCGGT A^CCGGT
PleI 1054 GAGTC GAGTC(4/5)
PpuMI 1100 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 249 ATTAAT AT^TAAT
PstI 466 CTGCAG CTGCA^G
SacI 430 GAGCTC GAGCT^C
SspBI 68 TGTACA T^GTACA
SspI 788 AATATT AAT^ATT
StuI 1114 AGGCCT AGG^CCT
StyI 913 CCATGG C^C[AT][AT]GG
Tsp45I 716 GTCAC ^GT[GC]AC
Van91I 932 CCACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1050 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BalI 346 TGGCCA TGG^CCA
458 TGGCCA
BanII 430 GAGCTC G[GA]GC[CT]^C
1175 GAGCCC
BbsI 82 GAAGAC GAAGAC(2/6)
166 GTCTTC
BbvI 256 GCTGC GCAGC(8/12)
465 GCTGC
BcnI 482 CCCGG CC^[GC]GG
1171 CCCGG
BfaI 161 CTAG C^TAG
1051 CTAG
BpuAI 82 GAAGAC GAAGAC(2/6)
166 GTCTTC
DsaI 261 CCACGG C^C[GA][CT]GG
913 CCATGG
EcoRI 119 GAATTC G^AATTC
1060 GAATTC
Fnu4HI 256 GCTGC GC^[ACGT]GC
465 GCTGC
ItaI 256 GCTGC GC^[ACGT]GC
465 GCTGC
MaeI 161 CTAG C^TAG
1051 CTAG
MluNI 346 TGGCCA TGG^CCA
458 TGGCCA
MscI 346 TGGCCA TGG^CCA
458 TGGCCA
MslI 60 CATGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
498 CATTTTCATG
MwoI 1168 GCTCCCGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1177 GCCCCCAGTGC
NciI 482 CCCGG CC^[GC]GG
1171 CCCGG
SfaNI 99 GATGC GCATC(5/9)
1261 GATGC
TseI 256 GCTGC G^C[AT]GC
465 GCTGC
XcmI 29 CCAGCAACGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
349 CCATCAAATTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI BmyI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EaeI EcoRII FokI HaeIII
HapII HinfI HpaII MaeII MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfcI
SmlI TfiI Tru9I Tsp509I TspRI
XhoII