Restriction map for
pF1KB5893
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BamHI
BanI BbeI BbrPI BbvCI BciVI
BfrI BlnI BsaAI BsaBI BsiEI
BsiWI BsmI Bsp1407I BspDI BspLU11I
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 1225 GACGTC GACGT^C
AccIII 1097 TCCGGA T^CCGGA
AlwNI 631 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 473 AGCGCT AGC^GCT
ApaI 1135 GGGCCC GGGCC^C
AspI 1075 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 44 CCCGGG C^[CT]CG[GA]G
AviII 218 TGCGCA TGC^GCA
BalI 732 TGGCCA TGG^CCA
BcgI 416 GCAACAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1046 TGATCA T^GATCA
BfaI 1486 CTAG C^TAG
BglI 253 GCCACGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 932 AGATCT A^GATCT
BpmI 1390 CTCCAG CTGGAG(16/14)
BsaI 784 GAGACC GGTCTC(1/5)
BseAI 1097 TCCGGA T^CCGGA
BsgI 610 CTGCAC GTGCAG(16/14)
BsoBI 44 CCCGGG C^[CT]CG[GA]G
BspEI 1097 TCCGGA T^CCGGA
BspHI 569 TCATGA T^CATGA
BsrDI 179 GCAATG GCAATG(2/0)
BstAPI 221 GCACTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 222 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 732 TGGCCA [CT]^GGCC[GA]
EarI 1246 GAAGAG CTCTTC(1/4)
Eco47III 473 AGCGCT AGC^GCT
FbaI 1046 TGATCA T^GATCA
FspI 218 TGCGCA TGC^GCA
HgaI 250 GACGC GACGC(5/10)
HindIII 538 AAGCTT A^AGCTT
Ksp632I 1246 GAAGAG CTCTTC(1/4)
MaeI 1486 CTAG C^TAG
MluNI 732 TGGCCA TGG^CCA
MroI 1097 TCCGGA T^CCGGA
MscI 732 TGGCCA TGG^CCA
MseI 803 TTAA T^TAA
PflFI 1075 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 630 CCAGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 653 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 360 GACGGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1480 CTGCAG CTGCA^G
RcaI 569 TCATGA T^CATGA
SmaI 44 CCCGGG CCC^GGG
SmlI 91 CTCAAG C^T[CT][GA]AG
Tru9I 803 TTAA T^TAA
Tsp509I 1104 AATT ^AATT
Tth111I 1075 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 630 CCAGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1232 CCAAGGCCTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 44 CCCGGG C^CCGGG
XmaI 44 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 250 GACGCC G[GA]^CG[CT]C
1225 GACGTC
AlwI 846 GATCC GGATC(4/5)
1162 GATCC
BlpI 34 GCTGAGC GC^T[ACGT]AGC
666 GCTGAGC
Bpu1102I 34 GCTGAGC GC^T[ACGT]AGC
666 GCTGAGC
BsaHI 250 GACGCC G[GA]^CG[CT]C
1225 GACGTC
BsaWI 614 ACCGGA [AT]^CCGG[AT]
1097 TCCGGA
BseRI 748 CTCCTC GAGGAG(10/8)
85 GAGGAG
BsmBI 248 GAGACG CGTCTC(1/5)
358 GAGACG
BsmFI 47 GGGAC GGGAC(10/14)
722 GGGAC
BspMI 215 ACCTGC ACCTGC(4/8)
1148 ACCTGC
BsrBI 445 GAGCGG CCGCTC(-3/-3)
1108 GAGCGG
BssSI 910 CACGAG CACGAG(-5/-1)
676 CTCGTG
BstXI 263 CCATTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
311 CCAGCAACATGG
BstYI 932 AGATCT [GA]^GATC[CT]
1161 AGATCC
CelII 34 GCTGAGC GC^T[ACGT]AGC
666 GCTGAGC
DraII 653 AGGTCCT [GA]G^G[ACGT]CC[CT]
1134 AGGGCCC
EcoO109I 653 AGGTCCT [GA]G^G[ACGT]CC[CT]
1134 AGGGCCC
HaeII 473 AGCGCT [GA]GCGC^[CT]
1181 GGCGCT
Hin1I 250 GACGCC G[GA]^CG[CT]C
1225 GACGTC
MaeII 1226 ACGT A^CGT
1448 ACGT
MflI 932 AGATCT [GA]^GATC[CT]
1161 AGATCC
MslI 672 CACGCTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1428 CACAGCAGTG
MspA1I 528 CAGCGG C[AC]G^C[GT]G
1320 CCGCTG
NcoI 729 CCATGG C^CATGG
1457 CCATGG
PleI 96 GACTC GAGTC(4/5)
487 GACTC
SfaNI 391 GATGC GCATC(5/9)
1028 GCATC
XhoII 932 AGATCT [GA]^GATC[CT]
1161 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BanII BbsI BbvI BcnI
BmyI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV RsaI
SacI Sau3AI Sau96I ScrFI SfcI
StuI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I