Restriction map for
pF1KB5905
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BsaAI BsaHI BsaI
BseAI BseRI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CfoI ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MaeII MluI MluNI MroI MscI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflMI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Van91I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AciI 1455 GCGG CCGC(-3/-1)
AcsI 1066 AAATTT [GA]^AATT[CT]
AflIII 1196 ACATGT A^C[GA][CT]GT
AgeI 1364 ACCGGT A^CCGGT
AlwI 565 GGATC GGATC(4/5)
ApoI 1066 AAATTT [GA]^AATT[CT]
Asp700I 690 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 552 GAGCTC G[AT]GC[AT]^C
AvaI 1109 CTCGAG C^[CT]CG[GA]G
AvrII 213 CCTAGG C^CTAGG
BbsI 118 GAAGAC GAAGAC(2/6)
BglII 993 AGATCT A^GATCT
BlnI 213 CCTAGG C^CTAGG
BlpI 601 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 601 GCTAAGC GC^T[ACGT]AGC
BpuAI 118 GAAGAC GAAGAC(2/6)
BsaBI 1238 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 552 GAGCTC G[AT]GC[AT]^C
BsoBI 1109 CTCGAG C^[CT]CG[GA]G
BspLU11I 1196 ACATGT A^CATGT
BsrDI 783 CATTGC GCAATG(2/0)
BsrFI 1364 ACCGGT [GA]^CCGG[CT]
BstXI 1360 CCAAACCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 993 AGATCT [GA]^GATC[CT]
Cac8I 715 GCCAGC GC[ACGT]^[ACGT]GC
CelII 601 GCTAAGC GC^T[ACGT]AGC
Cfr10I 1364 ACCGGT [GA]^CCGG[CT]
DraII 808 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoNI 1218 CCTTTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 808 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 793 GATATC GAT^ATC
EcoT14I 213 CCTAGG C^C[AT][AT]GG
FauI 1455 GCGGG CCCGC(4/6)
HgaI 889 GACGC GACGC(5/10)
MamI 1238 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 993 AGATCT [GA]^GATC[CT]
MspA1I 717 CAGCTG C[AC]G^C[GT]G
MwoI 1118 GCACCAAACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 1196 ACATGT [GA]CATG^[CT]
PaeR7I 1109 CTCGAG C^TCGAG
PinAI 1364 ACCGGT A^CCGGT
PpuMI 808 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 788 CTGCAG CTGCA^G
PvuII 717 CAGCTG CAG^CTG
SacI 552 GAGCTC GAGCT^C
ScaI 961 AGTACT AGT^ACT
SspI 133 AATATT AAT^ATT
StuI 570 AGGCCT AGG^CCT
StyI 213 CCTAGG C^C[AT][AT]GG
XhoI 1109 CTCGAG C^TCGAG
XhoII 993 AGATCT [GA]^GATC[CT]
XmnI 690 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspI 121 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
804 GACAGGGTC
BanI 34 GGCACC G^G[CT][GA]CC
1117 GGCACC
BanII 552 GAGCTC G[GA]GC[CT]^C
1421 GGGCTC
BpmI 1136 CTCCAG CTGGAG(16/14)
515 CTGGAG
BsaWI 443 TCCGGT [AT]^CCGG[AT]
1364 ACCGGT
BsgI 285 CTGCAC GTGCAG(16/14)
14 GTGCAG
BsmI 280 GAATGC GAATGC(1/-1)
1414 GAATGC
EarI 918 CTCTTC CTCTTC(1/4)
172 GAAGAG
HapII 444 CCGG C^CGG
1365 CCGG
HincII 127 GTCAAC GT[CT]^[GA]AC
1090 GTTGAC
HindII 127 GTCAAC GT[CT]^[GA]AC
1090 GTTGAC
HpaII 444 CCGG C^CGG
1365 CCGG
Ksp632I 918 CTCTTC CTCTTC(1/4)
172 GAAGAG
MfeI 275 CAATTG C^AATTG
980 CAATTG
MslI 844 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1011 CATTCTGGTG
MspI 444 CCGG C^CGG
1365 CCGG
MunI 275 CAATTG C^AATTG
980 CAATTG
PflFI 121 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
804 GACAGGGTC
SfaNI 1334 GCATC GCATC(5/9)
1435 GCATC
SmlI 965 CTCAAG C^T[CT][GA]AG
1109 CTCGAG
TfiI 520 GAATC G^A[AT]TC
609 GATTC
Tth111I 121 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
804 GACAGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AfaI AluI AvaII BbvI BfaI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MvaI NdeII
NlaIII NlaIV PleI RcaI RsaI
Sau3AI Sau96I ScrFI SfcI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I