Restriction map for
pF1KB5909
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflIII AgeI AhdI
Aor51HI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvrII BbeI
BcgI BlnI BpmI BsaHI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BspHI BspLU11I BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FseI HaeII HgaI
Hin1I HincII HindII HpaI KasI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tsp509I Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AflII 1270 CTTAAG C^TTAAG
Alw44I 1064 GTGCAC G^TGCAC
AocI 583 CCTGAGG CC^T[ACGT]AGG
ApaLI 1064 GTGCAC G^TGCAC
AviII 1262 TGCGCA TGC^GCA
BamHI 668 GGATCC G^GATCC
BbrPI 637 CACGTG CAC^GTG
BbsI 573 GAAGAC GAAGAC(2/6)
BciVI 49 GTATCC GTATCC(6/5)
BclI 978 TGATCA T^GATCA
BcnI 213 CCCGG CC^[GC]GG
BfaI 1187 CTAG C^TAG
BfrI 1270 CTTAAG C^TTAAG
BglI 1169 GCCACTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 609 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 609 GCTCAGC GC^T[ACGT]AGC
BpuAI 573 GAAGAC GAAGAC(2/6)
BsaAI 637 CACGTG [CT]AC^GT[GA]
BsaI 1039 GAGACC GGTCTC(1/5)
BsgI 259 CTGCAC GTGCAG(16/14)
BsmBI 355 CGTCTC CGTCTC(1/5)
BsmI 659 GCATTC GAATGC(1/-1)
Bsp1407I 894 TGTACA T^GTACA
BspMI 624 GCAGGT ACCTGC(4/8)
BsrBI 76 GAGCGG CCGCTC(-3/-3)
BsrFI 620 ACCGGC [GA]^CCGG[CT]
BsrGI 894 TGTACA T^GTACA
BstEII 798 GGTCACC G^GT[ACGT]ACC
BstPI 798 GGTCACC G^GT[ACGT]ACC
BstXI 1433 CCACAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 583 CCTGAGG CC^T[ACGT]AGG
CelII 609 GCTCAGC GC^T[ACGT]AGC
Cfr10I 620 ACCGGC [GA]^CCGG[CT]
DrdI 1237 GACTCCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 583 CCTGAGG CC^T[ACGT]AGG
EcoO65I 798 GGTCACC G^GT[ACGT]ACC
FbaI 978 TGATCA T^GATCA
FspI 1262 TGCGCA TGC^GCA
MaeI 1187 CTAG C^TAG
NciI 213 CCCGG CC^[GC]GG
NcoI 1106 CCATGG C^CATGG
PmaCI 637 CACGTG CAC^GTG
PmlI 637 CACGTG CAC^GTG
PpuMI 827 AGGACCT [GA]G^G[AT]CC[CT]
PstI 286 CTGCAG CTGCA^G
SapI 604 GAAGAGC GCTCTTC(1/4)
ScaI 983 AGTACT AGT^ACT
SexAI 830 ACCTGGT A^CC[AT]GGT
SfiI 1168 GGCCACTCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 894 TGTACA T^GTACA
StuI 930 AGGCCT AGG^CCT
TfiI 1401 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 31 enzymes) #
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Acc65I 112 GGTACC G^GTACC
1339 GGTACC
ApaI 908 GGGCCC GGGCC^C
1176 GGGCCC
Asp718I 112 GGTACC G^GTACC
1339 GGTACC
AvaI 380 CCCGAG C^[CT]CG[GA]G
1173 CTCGGG
BalI 1154 TGGCCA TGG^CCA
1167 TGGCCA
BanI 112 GGTACC G^G[CT][GA]CC
1339 GGTACC
BbvCI 197 CCTCAGC CCTCAGC(-5/-2)
933 CCTCAGC
BglII 1124 AGATCT A^GATCT
1373 AGATCT
BsaBI 1119 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1374 GATCTACATC
BsoBI 380 CCCGAG C^[CT]CG[GA]G
1173 CTCGGG
BsrDI 99 CATTGC GCAATG(2/0)
348 CATTGC
DraII 827 AGGACCT [GA]G^G[ACGT]CC[CT]
1176 GGGCCCC
DsaI 551 CCACGG C^C[GA][CT]GG
1106 CCATGG
EarI 564 GAAGAG CTCTTC(1/4)
604 GAAGAG
EcoO109I 827 AGGACCT [GA]G^G[ACGT]CC[CT]
1176 GGGCCCC
FauI 296 CCCGC CCCGC(4/6)
466 GCGGG
KpnI 112 GGTACC GGTAC^C
1339 GGTACC
Ksp632I 564 GAAGAG CTCTTC(1/4)
604 GAAGAG
MaeII 638 ACGT A^CGT
1143 ACGT
MamI 1119 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1374 GATCTACATC
MluNI 1154 TGGCCA TGG^CCA
1167 TGGCCA
MscI 1154 TGGCCA TGG^CCA
1167 TGGCCA
MseI 1271 TTAA T^TAA
1280 TTAA
PflMI 406 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
825 CCAGGACCTGG
PvuII 283 CAGCTG CAG^CTG
1291 CAGCTG
SacI 607 GAGCTC GAGCT^C
966 GAGCTC
SmlI 1270 CTTAAG C^T[CT][GA]AG
1495 CTCAAG
TaqI 89 TCGA T^CGA
158 TCGA
Tru9I 1271 TTAA T^TAA
1280 TTAA
TthHB8I 89 TCGA T^CGA
158 TCGA
Van91I 406 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
825 CCAGGACCTGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanII BbvI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
TspRI XhoII