Restriction map for
pF1KB5925
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BfaI BfrI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HgaI Hin1I HpaI KasI KpnI
MaeI MaeII MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SspI SwaI TfiI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AlwI 244 GATCC GGATC(4/5)
Aor51HI 947 AGCGCT AGC^GCT
Asp700I 120 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1473 TGGCCA TGG^CCA
BanI 1272 GGTGCC G^G[CT][GA]CC
BcgI 959 CGAACCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1337 GTATCC GTATCC(6/5)
BclI 734 TGATCA T^GATCA
BglI 241 GCCGATCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmAI 208 GAGAC GTCTC(1/5)
Bsp1407I 1263 TGTACA T^GTACA
BsrBI 1056 GAGCGG CCGCTC(-3/-3)
BsrFI 833 GCCGGC [GA]^CCGG[CT]
BsrGI 1263 TGTACA T^GTACA
BstAPI 1422 GCAGCGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 833 GCCGGC [GA]^CCGG[CT]
Eco47III 947 AGCGCT AGC^GCT
EcoNI 1453 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 734 TGATCA T^GATCA
HaeII 947 AGCGCT [GA]GCGC^[CT]
HincII 106 GTCAAC GT[CT]^[GA]AC
HindII 106 GTCAAC GT[CT]^[GA]AC
HindIII 1120 AAGCTT A^AGCTT
KspI 984 CCGCGG CCGC^GG
MaeIII 938 GTGAC ^GT[ACGT]AC
MluNI 1473 TGGCCA TGG^CCA
MscI 1473 TGGCCA TGG^CCA
MseI 281 TTAA T^TAA
MslI 12 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 833 GCCGGC GCC^GGC
NcoI 825 CCATGG C^CATGG
NgoMIV 833 GCCGGC G^CCGGC
PflMI 963 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 984 CCGCGG CCGC^GG
SbfI 703 CCTGCAGG CCTGCA^GG
Sse8387I 703 CCTGCAGG CCTGCA^GG
SspBI 1263 TGTACA T^GTACA
Tru9I 281 TTAA T^TAA
Tsp45I 938 GTGAC ^GT[GC]AC
Van91I 963 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 120 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 40 CGCG CG^CG
985 CGCG
AlwNI 715 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
964 CAGCTGCTG
AocI 397 CCTGAGG CC^T[ACGT]AGG
922 CCTGAGG
AspHI 1150 GAGCTC G[AT]GC[AT]^C
1396 GAGCTC
BbvCI 334 CCTCAGC CCTCAGC(-5/-2)
1070 CCTCAGC
BsgI 416 GTGCAG GTGCAG(16/14)
487 GTGCAG
BsiHKAI 1150 GAGCTC G[AT]GC[AT]^C
1396 GAGCTC
BstUI 40 CGCG CG^CG
985 CGCG
Bsu36I 397 CCTGAGG CC^T[ACGT]AGG
922 CCTGAGG
Eco81I 397 CCTGAGG CC^T[ACGT]AGG
922 CCTGAGG
HinfI 385 GACTC G^A[ACGT]TC
1081 GACTC
HphI 72 GGTGA GGTGA(8/7)
809 TCACC
MvnI 40 CGCG CG^CG
985 CGCG
PleI 385 GACTC GAGTC(4/5)
1081 GACTC
SacI 1150 GAGCTC GAGCT^C
1396 GAGCTC
SapI 442 GAAGAGC GCTCTTC(1/4)
837 GCTCTTC
SfaNI 481 GATGC GCATC(5/9)
131 GCATC
SmaI 320 CCCGGG CCC^GGG
800 CCCGGG
SphI 144 GCATGC GCATG^C
1505 GCATGC
StuI 194 AGGCCT AGG^CCT
1461 AGGCCT
TaqI 1196 TCGA T^CGA
1287 TCGA
Tsp509I 122 AATT ^AATT
1388 AATT
TthHB8I 1196 TCGA T^CGA
1287 TCGA
XcmI 91 CCAGGGCTTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1443 CCAGGCCCCTCCTGG
XmaCI 320 CCCGGG C^CCGGG
800 CCCGGG
XmaI 320 CCCGGG C^CCGGG
800 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI ApaI AvaI
AvaII BanII BbvI BcnI BglII
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI Ksp632I MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TseI TspRI XhoII