##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BbrPI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DraIII
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MaeII
MamI MfeI MluI MluNI MroI
MscI MunI NdeI NheI NotI
NruI NsiI NspI PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcyI 48 GGCGCC G[GA]^CG[CT]C
ApaI 1602 GGGCCC GGGCC^C
AvaI 190 CTCGAG C^[CT]CG[GA]G
BamHI 1112 GGATCC G^GATCC
BanI 48 GGCGCC G^G[CT][GA]CC
BbeI 48 GGCGCC GGCGC^C
BbsI 885 GAAGAC GAAGAC(2/6)
BglI 515 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1319 AGATCT A^GATCT
BpuAI 885 GAAGAC GAAGAC(2/6)
BsaHI 48 GGCGCC G[GA]^CG[CT]C
BsaWI 9 ACCGGA [AT]^CCGG[AT]
BsoBI 190 CTCGAG C^[CT]CG[GA]G
BspHI 684 TCATGA T^CATGA
BspMI 1380 GCAGGT ACCTGC(4/8)
BsrBI 1375 GAGCGG CCGCTC(-3/-3)
BsrDI 1073 GCAATG GCAATG(2/0)
BstBI 795 TTCGAA TT^CGAA
DrdI 757 GACCAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 234 CGGCCG C^GGCCG
EclXI 234 CGGCCG C^GGCCG
Eco52I 234 CGGCCG C^GGCCG
HgaI 13 GACGC GACGC(5/10)
Hin1I 48 GGCGCC G[GA]^CG[CT]C
HphI 1054 TCACC GGTGA(8/7)
KasI 48 GGCGCC G^GCGCC
KspI 156 CCGCGG CCGC^GG
MslI 1452 CACCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 45 GCCGGC GCC^GGC
NarI 48 GGCGCC GG^CGCC
NgoMIV 45 GCCGGC G^CCGGC
NspV 795 TTCGAA TT^CGAA
PaeR7I 190 CTCGAG C^TCGAG
PflMI 1521 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 684 TCATGA T^CATGA
SacII 156 CCGCGG CCGC^GG
SfoI 48 GGCGCC GGC^GCC
SfuI 795 TTCGAA TT^CGAA
SmlI 190 CTCGAG C^T[CT][GA]AG
Tsp509I 413 AATT ^AATT
Van91I 1521 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1448 CCACCACCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 190 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwI 1113 GATCC GGATC(4/5)
1112 GGATC
AspHI 136 GTGCTC G[AT]GC[AT]^C
1216 GTGCTC
BsgI 1032 CTGCAC GTGCAG(16/14)
220 GTGCAG
BsiEI 215 CGGTCG CG[GA][CT]^CG
234 CGGCCG
BsiHKAI 136 GTGCTC G[AT]GC[AT]^C
1216 GTGCTC
BsrFI 45 GCCGGC [GA]^CCGG[CT]
213 GCCGGT
BstAPI 90 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
873 GCAGCAGCTGC
BstF5I 804 GGATG GGATG(2/0)
918 GGATG
BstYI 1112 GGATCC [GA]^GATC[CT]
1319 AGATCT
Cfr10I 45 GCCGGC [GA]^CCGG[CT]
213 GCCGGT
EcoNI 84 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1562 CCTTCCTGAGG
FokI 804 GGATG GGATG(9/13)
918 GGATG
MflI 1112 GGATCC [GA]^GATC[CT]
1319 AGATCT
NcoI 446 CCATGG C^CATGG
1457 CCATGG
TfiI 718 GAATC G^A[AT]TC
798 GAATC
Tsp45I 711 GTCAC ^GT[GC]AC
1365 GTCAC
XhoII 1112 GGATCC [GA]^GATC[CT]
1319 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AluI AlwNI AocI
AvaII BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI BstUI
BstXI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PshAI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
TspRI TthHB8I