################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BbrPI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BssSI Bst1107I BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeII MamI MfeI MluI MluNI MroI MscI MunI NdeI NheI NotI NruI NsiI NspI PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcyI 48 GGCGCC G[GA]^CG[CT]C ApaI 1602 GGGCCC GGGCC^C AvaI 190 CTCGAG C^[CT]CG[GA]G BamHI 1112 GGATCC G^GATCC BanI 48 GGCGCC G^G[CT][GA]CC BbeI 48 GGCGCC GGCGC^C BbsI 885 GAAGAC GAAGAC(2/6) BglI 515 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1319 AGATCT A^GATCT BpuAI 885 GAAGAC GAAGAC(2/6) BsaHI 48 GGCGCC G[GA]^CG[CT]C BsaWI 9 ACCGGA [AT]^CCGG[AT] BsoBI 190 CTCGAG C^[CT]CG[GA]G BspHI 684 TCATGA T^CATGA BspMI 1380 GCAGGT ACCTGC(4/8) BsrBI 1375 GAGCGG CCGCTC(-3/-3) BsrDI 1073 GCAATG GCAATG(2/0) BstBI 795 TTCGAA TT^CGAA DrdI 757 GACCAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 234 CGGCCG C^GGCCG EclXI 234 CGGCCG C^GGCCG Eco52I 234 CGGCCG C^GGCCG HgaI 13 GACGC GACGC(5/10) Hin1I 48 GGCGCC G[GA]^CG[CT]C HphI 1054 TCACC GGTGA(8/7) KasI 48 GGCGCC G^GCGCC KspI 156 CCGCGG CCGC^GG MslI 1452 CACCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 45 GCCGGC GCC^GGC NarI 48 GGCGCC GG^CGCC NgoMIV 45 GCCGGC G^CCGGC NspV 795 TTCGAA TT^CGAA PaeR7I 190 CTCGAG C^TCGAG PflMI 1521 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 684 TCATGA T^CATGA SacII 156 CCGCGG CCGC^GG SfoI 48 GGCGCC GGC^GCC SfuI 795 TTCGAA TT^CGAA SmlI 190 CTCGAG C^T[CT][GA]AG Tsp509I 413 AATT ^AATT Van91I 1521 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1448 CCACCACCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 190 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwI 1113 GATCC GGATC(4/5) 1112 GGATC AspHI 136 GTGCTC G[AT]GC[AT]^C 1216 GTGCTC BsgI 1032 CTGCAC GTGCAG(16/14) 220 GTGCAG BsiEI 215 CGGTCG CG[GA][CT]^CG 234 CGGCCG BsiHKAI 136 GTGCTC G[AT]GC[AT]^C 1216 GTGCTC BsrFI 45 GCCGGC [GA]^CCGG[CT] 213 GCCGGT BstAPI 90 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 873 GCAGCAGCTGC BstF5I 804 GGATG GGATG(2/0) 918 GGATG BstYI 1112 GGATCC [GA]^GATC[CT] 1319 AGATCT Cfr10I 45 GCCGGC [GA]^CCGG[CT] 213 GCCGGT EcoNI 84 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1562 CCTTCCTGAGG FokI 804 GGATG GGATG(9/13) 918 GGATG MflI 1112 GGATCC [GA]^GATC[CT] 1319 AGATCT NcoI 446 CCATGG C^CATGG 1457 CCATGG TfiI 718 GAATC G^A[AT]TC 798 GAATC Tsp45I 711 GTCAC ^GT[GC]AC 1365 GTCAC XhoII 1112 GGATCC [GA]^GATC[CT] 1319 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AccII AciI AluI AlwNI AocI AvaII BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI BstUI BstXI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PshAI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI TspRI TthHB8I