Restriction map for
pF1KB5935
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AvaI AviII
AvrII BalI BanI BanII BbeI
BbrPI BbsI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaI BsaJI
BsaWI BseAI BseRI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspMI BsrDI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRI EcoT14I FbaI FseI
FspI HaeII HapII HindIII HpaII
HphI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI StyI
SwaI Van91I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AflIII 296 ACATGT A^C[GA][CT]GT
AocI 950 CCTCAGG CC^T[ACGT]AGG
AseI 70 ATTAAT AT^TAAT
AsnI 70 ATTAAT AT^TAAT
AspI 31 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1117 GGACC G^G[AT]CC
BamHI 714 GGATCC G^GATCC
BbvCI 565 GCTGAGG CCTCAGC(-5/-2)
BcgI 1428 CGAGAGAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsgI 278 GTGCAG GTGCAG(16/14)
Bsp1407I 1243 TGTACA T^GTACA
BspLU11I 296 ACATGT A^CATGT
BsrBI 613 GAGCGG CCGCTC(-3/-3)
BsrGI 1243 TGTACA T^GTACA
BssSI 1469 CACGAG CACGAG(-5/-1)
Bsu36I 950 CCTCAGG CC^T[ACGT]AGG
CfoI 1398 GCGC GCG^C
Eco81I 950 CCTCAGG CC^T[ACGT]AGG
EcoNI 526 CCTTCAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1165 ATGCAT ATGCA^T
HaeIII 761 GGCC GG^CC
HhaI 1398 GCGC GCG^C
HinP1I 1398 GCGC G^CGC
HpaI 901 GTTAAC GTT^AAC
MaeII 153 ACGT A^CGT
NlaIV 714 GGATCC GG[ACGT]^[ACGT]CC
NsiI 1165 ATGCAT ATGCA^T
PflFI 31 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 70 ATTAAT AT^TAAT
ScaI 1507 AGTACT AGT^ACT
SfcI 596 CTACAG C^T[GA][CT]AG
SspBI 1243 TGTACA T^GTACA
SspI 1223 AATATT AAT^ATT
Tth111I 31 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1155 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 748 AAATTT [GA]^AATT[CT]
1449 AAATTT
AcyI 1232 GGCGTC G[GA]^CG[CT]C
1274 GACGCC
ApoI 748 AAATTT [GA]^AATT[CT]
1449 AAATTT
AspHI 515 GTGCTC G[AT]GC[AT]^C
1208 GTGCTC
BpmI 413 CTCCAG CTGGAG(16/14)
802 CTGGAG
BsaBI 43 GATAACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1419 GATAGACATC
BsaHI 1232 GGCGTC G[GA]^CG[CT]C
1274 GACGCC
BsiHKAI 515 GTGCTC G[AT]GC[AT]^C
1208 GTGCTC
BssKI 415 CCAGG ^CC[ACGT]GG
1394 CCAGG
BstNI 415 CCAGG CC^[AT]GG
1394 CCAGG
BstYI 714 GGATCC [GA]^GATC[CT]
1158 AGATCC
Cfr13I 760 GGGCC G^G[ACGT]CC
1117 GGACC
EcoRII 415 CCAGG ^CC[AT]GG
1394 CCAGG
FauI 146 CCCGC CCCGC(4/6)
1082 CCCGC
Hin1I 1232 GGCGTC G[GA]^CG[CT]C
1274 GACGCC
MamI 43 GATAACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1419 GATAGACATC
MflI 714 GGATCC [GA]^GATC[CT]
1158 AGATCC
MvaI 415 CCAGG CC^[AT]GG
1394 CCAGG
NspI 296 ACATGT [GA]CATG^[CT]
636 GCATGT
SapI 340 GAAGAGC GCTCTTC(1/4)
610 GAAGAGC
Sau96I 760 GGGCC G^G[ACGT]CC
1117 GGACC
ScrFI 415 CCAGG CC^[ACGT]GG
1394 CCAGG
SfaNI 472 GATGC GCATC(5/9)
889 GATGC
Tsp45I 155 GTGAC ^GT[GC]AC
1334 GTGAC
XhoII 714 GGATCC [GA]^GATC[CT]
1158 AGATCC
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BfaI BmyI BsiYI BslI BsmFI
Bsp1286I BsrI BstF5I Cac8I DdeI
DpnII EarI EcoRV Fnu4HI FokI
HgaI HincII HindII HinfI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MslI MwoI NdeII
NlaIII PleI RsaI Sau3AI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I