Restriction map for
pF1KB5973
##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BamHI
BbvCI BcgI BclI BfaI BfrI
BglII BlnI BsaBI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI Bst1107I
BstBI BstXI BstZ17I ClaI CpoI
DraI DraIII Eam1105I EarI Eco47III
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
Ksp632I MaeI MamI MfeI MluI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SfaNI SfiI SfuI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I XbaI XcmI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AfaI 393 GTAC GT^AC
AspI 1318 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1399 CCCGGG C^[CT]CG[GA]G
BbrPI 72 CACGTG CAC^GTG
BbsI 1324 GTCTTC GAAGAC(2/6)
BciVI 19 GGATAC GTATCC(6/5)
BlpI 1090 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1090 GCTAAGC GC^T[ACGT]AGC
BpuAI 1324 GTCTTC GAAGAC(2/6)
BsgI 1445 CTGCAC GTGCAG(16/14)
BsoBI 1399 CCCGGG C^[CT]CG[GA]G
BssSI 184 CACGAG CACGAG(-5/-1)
BstAPI 71 GCACGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1293 GGTAACC G^GT[ACGT]ACC
BstPI 1293 GGTAACC G^GT[ACGT]ACC
BstYI 325 GGATCT [GA]^GATC[CT]
CelII 1090 GCTAAGC GC^T[ACGT]AGC
DrdI 880 GACGGCCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1293 GGTAACC G^GT[ACGT]ACC
HphI 542 TCACC GGTGA(8/7)
MflI 325 GGATCT [GA]^GATC[CT]
MslI 543 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 416 CCATGG C^CATGG
NspI 1178 GCATGC [GA]CATG^[CT]
PflFI 1318 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 837 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 72 CACGTG CAC^GTG
PmlI 72 CACGTG CAC^GTG
PshAI 847 GACGGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1493 CAGCTG CAG^CTG
RsaI 393 GTAC GT^AC
SexAI 571 ACCTGGT A^CC[AT]GGT
SmaI 1399 CCCGGG CCC^GGG
SphI 1178 GCATGC GCATG^C
TaqI 788 TCGA T^CGA
TfiI 307 GATTC G^A[AT]TC
TspRI 1007 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 1318 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 788 TCGA T^CGA
Van91I 837 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 325 GGATCT [GA]^GATC[CT]
XmaCI 1399 CCCGGG C^CCGGG
XmaI 1399 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 139 GTCTAC GT^[AC][GT]AC
151 GTCTAC
AccIII 85 TCCGGA T^CCGGA
911 TCCGGA
AlwI 235 GGATC GGATC(4/5)
325 GGATC
AlwNI 350 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1305 CAGAAACTG
ApaI 112 GGGCCC GGGCC^C
965 GGGCCC
AviII 330 TGCGCA TGC^GCA
640 TGCGCA
BglI 118 GCCGCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1204 GCCCCTGCGGC
BsaAI 72 CACGTG [CT]AC^GT[GA]
256 TACGTG
BsaI 454 GAGACC GGTCTC(1/5)
564 GGTCTC
BsaWI 85 TCCGGA [AT]^CCGG[AT]
911 TCCGGA
BseAI 85 TCCGGA T^CCGGA
911 TCCGGA
BsiEI 197 CGGCCG CG[GA][CT]^CG
992 CGGCCG
BspEI 85 TCCGGA T^CCGGA
911 TCCGGA
EagI 197 CGGCCG C^GGCCG
992 CGGCCG
EclXI 197 CGGCCG C^GGCCG
992 CGGCCG
Eco52I 197 CGGCCG C^GGCCG
992 CGGCCG
FspI 330 TGCGCA TGC^GCA
640 TGCGCA
MboII 522 GAAGA GAAGA(8/7)
1325 TCTTC
MroI 85 TCCGGA T^CCGGA
911 TCCGGA
PstI 481 CTGCAG CTGCA^G
1496 CTGCAG
SacI 187 GAGCTC GAGCT^C
720 GAGCTC
SfcI 481 CTGCAG C^T[GA][CT]AG
1496 CTGCAG
SgrAI 589 CGCCGGCG C[GA]^CCGG[CT]G
809 CGCCGGCG
Tsp45I 259 GTGAC ^GT[GC]AC
773 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AluI AocI
AspHI AvaII BalI BanI BanII
BbeI BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI KasI
KspI MaeII MaeIII MboI MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI SacII Sau3AI Sau96I ScrFI
SfoI SmlI StuI StyI TseI