Restriction map for
pF1KB6102
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbsI BclI
BfrI BglII BlnI BpuAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraII DraIII Eam1105I Eco47III
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FbaI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MluI MroI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Alw44I 715 GTGCAC G^TGCAC
AocI 44 CCTCAGG CC^T[ACGT]AGG
ApaLI 715 GTGCAC G^TGCAC
Asp700I 652 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 1321 GGATAC GTATCC(6/5)
BfaI 964 CTAG C^TAG
BglI 1682 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 643 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 643 GCTCAGC GC^T[ACGT]AGC
BsaAI 1324 TACGTA [CT]AC^GT[GA]
BsaI 1239 GGTCTC GGTCTC(1/5)
BsiEI 1699 CGGCCG CG[GA][CT]^CG
BsmFI 1228 GGGAC GGGAC(10/14)
BspHI 860 TCATGA T^CATGA
BsrBI 1639 GAGCGG CCGCTC(-3/-3)
BsrFI 1697 GCCGGC [GA]^CCGG[CT]
BstYI 154 AGATCC [GA]^GATC[CT]
Bsu36I 44 CCTCAGG CC^T[ACGT]AGG
CelII 643 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1697 GCCGGC [GA]^CCGG[CT]
DrdI 989 GACACCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1699 CGGCCG C^GGCCG
EclXI 1699 CGGCCG C^GGCCG
Eco52I 1699 CGGCCG C^GGCCG
Eco81I 44 CCTCAGG CC^T[ACGT]AGG
EcoNI 1727 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 499 GAATTC G^AATTC
FauI 588 GCGGG CCCGC(4/6)
FseI 1696 GGCCGGCC GGCCGG^CC
MaeI 964 CTAG C^TAG
MflI 154 AGATCC [GA]^GATC[CT]
MseI 235 TTAA T^TAA
NaeI 1697 GCCGGC GCC^GGC
NgoMIV 1697 GCCGGC G^CCGGC
NspI 1579 ACATGC [GA]CATG^[CT]
PflMI 633 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 860 TCATGA T^CATGA
SmaI 868 CCCGGG CCC^GGG
SmlI 562 CTCAAG C^T[CT][GA]AG
SnaBI 1324 TACGTA TAC^GTA
Tru9I 235 TTAA T^TAA
Van91I 633 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1647 CCACCGCCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 154 AGATCC [GA]^GATC[CT]
XmaCI 868 CCCGGG C^CCGGG
XmaI 868 CCCGGG C^CCGGG
XmnI 652 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 499 GAATTC [GA]^AATT[CT]
1376 AAATTC
AlwI 155 GATCC GGATC(4/5)
124 GGATC
ApoI 499 GAATTC [GA]^AATT[CT]
1376 AAATTC
AvaI 608 CTCGGG C^[CT]CG[GA]G
868 CCCGGG
BalI 18 TGGCCA TGG^CCA
1674 TGGCCA
BanII 1680 GAGCCC G[GA]GC[CT]^C
1722 GGGCTC
BbvCI 1215 GCTGAGG CCTCAGC(-5/-2)
1411 GCTGAGG
BcgI 600 GCACTGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1783 GCAACCACATCG
BcnI 868 CCCGG CC^[GC]GG
869 CCGGG
BpmI 1242 CTCCAG CTGGAG(16/14)
197 CTGGAG
BsoBI 608 CTCGGG C^[CT]CG[GA]G
868 CCCGGG
BstXI 72 CCAACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
993 CCACAGTCTTGG
DsaI 1538 CCACGG C^C[GA][CT]GG
1686 CCACGG
HaeII 1127 GGCGCT [GA]GCGC^[CT]
1148 GGCGCT
HapII 869 CCGG C^CGG
1698 CCGG
HpaII 869 CCGG C^CGG
1698 CCGG
MaeII 75 ACGT A^CGT
1325 ACGT
MaeIII 487 GTGAC ^GT[ACGT]AC
1816 GTGAC
MluNI 18 TGGCCA TGG^CCA
1674 TGGCCA
MscI 18 TGGCCA TGG^CCA
1674 TGGCCA
MslI 708 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
718 CACGGAAATG
MspA1I 586 CAGCGG C[AC]G^C[GT]G
1702 CCGCTG
MspI 869 CCGG C^CGG
1698 CCGG
NciI 868 CCCGG CC^[GC]GG
869 CCGGG
PstI 583 CTGCAG CTGCA^G
1746 CTGCAG
Tsp45I 487 GTGAC ^GT[GC]AC
1816 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BanI BbvI BmyI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EarI EcoRII Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MboI MboII
MfeI MnlI MunI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI TseI Tsp509I
TspRI TthHB8I