Restriction map for
pF1KB6134
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BbrPI BfrI BglII BlnI BsaI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV FseI
HincII HindII HindIII HpaI KpnI
KspI MluI MroI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 954 GACGTC GACGT^C
AccI 625 GTCTAC GT^[AC][GT]AC
AocI 1858 CCTCAGG CC^T[ACGT]AGG
AviII 8 TGCGCA TGC^GCA
BalI 611 TGGCCA TGG^CCA
BbvCI 603 CCTCAGC CCTCAGC(-5/-2)
BcgI 929 CGATCCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 199 GGATAC GTATCC(6/5)
BclI 404 TGATCA T^GATCA
BfaI 1330 CTAG C^TAG
BlpI 534 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 534 GCTCAGC GC^T[ACGT]AGC
BsaAI 864 CACGTA [CT]AC^GT[GA]
BsaBI 924 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1680 TCCGGT [AT]^CCGG[AT]
BsgI 124 CTGCAC GTGCAG(16/14)
BsmAI 76 GTCTC GTCTC(1/5)
Bsp1407I 113 TGTACA T^GTACA
BspMI 431 ACCTGC ACCTGC(4/8)
BsrDI 1799 GCAATG GCAATG(2/0)
BsrGI 113 TGTACA T^GTACA
BssSI 619 CACGAG CACGAG(-5/-1)
BstEII 1683 GGTCACC G^GT[ACGT]ACC
BstPI 1683 GGTCACC G^GT[ACGT]ACC
Bsu36I 1858 CCTCAGG CC^T[ACGT]AGG
CelII 534 GCTCAGC GC^T[ACGT]AGC
DsaI 143 CCGTGG C^C[GA][CT]GG
Eco81I 1858 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1683 GGTCACC G^GT[ACGT]ACC
EcoT14I 1025 CCAAGG C^C[AT][AT]GG
EcoT22I 1651 ATGCAT ATGCA^T
FbaI 404 TGATCA T^GATCA
FspI 8 TGCGCA TGC^GCA
MaeI 1330 CTAG C^TAG
MamI 924 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1658 CAATTG C^AATTG
MluNI 611 TGGCCA TGG^CCA
MscI 611 TGGCCA TGG^CCA
MseI 257 TTAA T^TAA
MunI 1658 CAATTG C^AATTG
NsiI 1651 ATGCAT ATGCA^T
PaeR7I 1186 CTCGAG C^TCGAG
PleI 1735 GACTC GAGTC(4/5)
SmaI 1217 CCCGGG CCC^GGG
SspBI 113 TGTACA T^GTACA
StyI 1025 CCAAGG C^C[AT][AT]GG
Tru9I 257 TTAA T^TAA
XhoI 1186 CTCGAG C^TCGAG
XmaCI 1217 CCCGGG C^CCGGG
XmaI 1217 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Aor51HI 830 AGCGCT AGC^GCT
1442 AGCGCT
AvaI 1186 CTCGAG C^[CT]CG[GA]G
1217 CCCGGG
BamHI 1765 GGATCC G^GATCC
1875 GGATCC
BbeI 1013 GGCGCC GGCGC^C
1151 GGCGCC
BbsI 849 GAAGAC GAAGAC(2/6)
951 GAAGAC
BpuAI 849 GAAGAC GAAGAC(2/6)
951 GAAGAC
BsoBI 1186 CTCGAG C^[CT]CG[GA]G
1217 CCCGGG
BsrFI 1553 ACCGGC [GA]^CCGG[CT]
1643 ACCGGC
BstXI 917 CCATCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1379 CCAGACTGCTGG
BstYI 1765 GGATCC [GA]^GATC[CT]
1875 GGATCC
Cfr10I 1553 ACCGGC [GA]^CCGG[CT]
1643 ACCGGC
Eco47III 830 AGCGCT AGC^GCT
1442 AGCGCT
KasI 1013 GGCGCC G^GCGCC
1151 GGCGCC
MaeIII 1684 GTCAC ^GT[ACGT]AC
1733 GTGAC
MflI 1765 GGATCC [GA]^GATC[CT]
1875 GGATCC
NarI 1013 GGCGCC GG^CGCC
1151 GGCGCC
NspI 203 ACATGC [GA]CATG^[CT]
836 GCATGT
PpuMI 968 AGGACCT [GA]G^G[AT]CC[CT]
1780 AGGACCC
SacI 556 GAGCTC GAGCT^C
634 GAGCTC
SapI 1474 GAAGAGC GCTCTTC(1/4)
558 GCTCTTC
SfiI 498 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
507 GGCCCAGGTGGCC
SfoI 1013 GGCGCC GGC^GCC
1151 GGCGCC
TaqI 1187 TCGA T^CGA
1451 TCGA
TfiI 1357 GAATC G^A[AT]TC
1367 GAATC
Tsp45I 1684 GTCAC ^GT[GC]AC
1733 GTGAC
Tsp509I 565 AATT ^AATT
1659 AATT
TspRI 793 CACTG CA[GC]TG[ACGT][ACGT]^
1624 CACTG
TthHB8I 1187 TCGA T^CGA
1451 TCGA
XhoII 1765 GGATCC [GA]^GATC[CT]
1875 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrBI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TseI