Restriction map for
pF1KB6627
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI Asp718I AspEI AspHI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BssHII BssSI BstBI
BstEII BstPI BstUI Bsu36I Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I StuI SwaI Tsp45I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 991 GACGCC G[GA]^CG[CT]C
AlwNI 1106 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 505 ATTAAT AT^TAAT
AsnI 505 ATTAAT AT^TAAT
AspI 428 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 132 GGGCTC G[GA]GC[CT]^C
BcnI 411 CCGGG CC^[GC]GG
BlpI 679 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 679 GCTCAGC GC^T[ACGT]AGC
BsaHI 991 GACGCC G[GA]^CG[CT]C
BsgI 482 GTGCAG GTGCAG(16/14)
BsmFI 1005 GGGAC GGGAC(10/14)
BsmI 597 GAATGC GAATGC(1/-1)
Bsp1407I 693 TGTACA T^GTACA
BsrBI 321 CCGCTC CCGCTC(-3/-3)
BsrGI 693 TGTACA T^GTACA
Bst1107I 919 GTATAC GTA^TAC
BstZ17I 919 GTATAC GTA^TAC
CelII 679 GCTCAGC GC^T[ACGT]AGC
CfoI 684 GCGC GCG^C
HapII 411 CCGG C^CGG
HgaI 991 GACGC GACGC(5/10)
HhaI 684 GCGC GCG^C
Hin1I 991 GACGCC G[GA]^CG[CT]C
HinP1I 684 GCGC G^CGC
HpaII 411 CCGG C^CGG
MspI 411 CCGG C^CGG
NciI 411 CCGGG CC^[GC]GG
NdeI 770 CATATG CA^TATG
NspI 788 GCATGT [GA]CATG^[CT]
PflFI 428 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 505 ATTAAT AT^TAAT
PstI 296 CTGCAG CTGCA^G
PvuII 231 CAGCTG CAG^CTG
SapI 943 GAAGAGC GCTCTTC(1/4)
SspBI 693 TGTACA T^GTACA
SspI 440 AATATT AAT^ATT
TaqI 426 TCGA T^CGA
TfiI 756 GATTC G^A[AT]TC
Tth111I 428 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 426 TCGA T^CGA
XbaI 759 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AciI 321 CCGC CCGC(-3/-1)
1109 CCGC
AfaI 694 GTAC GT^AC
705 GTAC
Asp700I 78 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
418 GAATATATTC
AvaII 588 GGTCC G^G[AT]CC
881 GGACC
BalI 116 TGGCCA TGG^CCA
161 TGGCCA
BbsI 151 GAAGAC GAAGAC(2/6)
739 GAAGAC
BciVI 576 GGATAC GTATCC(6/5)
730 GGATAC
BmyI 132 GGGCTC G[AGT]GC[ACT]^C
1095 GGGCAC
BpuAI 151 GAAGAC GAAGAC(2/6)
739 GAAGAC
BseRI 447 CTCCTC GAGGAG(10/8)
1090 GAGGAG
Bsp1286I 132 GGGCTC G[AGT]GC[ACT]^C
1095 GGGCAC
BstAPI 289 GCACAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
337 GCAATCAATGC
BstXI 113 CCATGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
827 CCACTGGCTTGG
EaeI 116 TGGCCA [CT]^GGCC[GA]
161 TGGCCA
EarI 82 CTCTTC CTCTTC(1/4)
943 GAAGAG
EcoT22I 344 ATGCAT ATGCA^T
1195 ATGCAT
Ksp632I 82 CTCTTC CTCTTC(1/4)
943 GAAGAG
MaeIII 202 GTTAC ^GT[ACGT]AC
552 GTTAC
MluNI 116 TGGCCA TGG^CCA
161 TGGCCA
MscI 116 TGGCCA TGG^CCA
161 TGGCCA
MseI 179 TTAA T^TAA
506 TTAA
MslI 114 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
978 CATGGGCATG
MspA1I 231 CAGCTG C[AC]G^C[GT]G
1109 CCGCTG
NlaIV 133 GGCTCC GG[ACGT]^[ACGT]CC
190 GGAGCC
NsiI 344 ATGCAT ATGCA^T
1195 ATGCAT
PflMI 769 CCATATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1068 CCAAGCCATGG
RsaI 694 GTAC GT^AC
705 GTAC
SfcI 296 CTGCAG C^T[GA][CT]AG
566 CTGTAG
Tru9I 179 TTAA T^TAA
506 TTAA
TspRI 828 CACTG CA[GC]TG[ACGT][ACGT]^
1098 CACTG
Van91I 769 CCATATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1068 CCAAGCCATGG
XmnI 78 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
418 GAATATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccI AluI AlwI BbvI BfaI
BpmI BsaJI BsiYI BslI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MboI MboII
MflI MnlI MvaI MwoI NcoI
NdeII NlaIII PleI Sau3AI Sau96I
ScrFI SfaNI StyI TseI Tsp509I
XcmI XhoII