Restriction map for
pF1KB6635
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI KpnI MaeII
MamI MfeI MluI MluNI MroI
MscI MunI NarI NcoI NdeI
NheI NlaIV NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
StyI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflII 933 CTTAAG C^TTAAG
Asp700I 776 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 667 GAGCAC G[AT]GC[AT]^C
AvaII 81 GGACC G^G[AT]CC
BcnI 60 CCCGG CC^[GC]GG
BfrI 933 CTTAAG C^TTAAG
BglII 108 AGATCT A^GATCT
BmyI 667 GAGCAC G[AGT]GC[ACT]^C
BsiHKAI 667 GAGCAC G[AT]GC[AT]^C
BsmAI 1247 GAGAC GTCTC(1/5)
BsmI 1150 GCATTC GAATGC(1/-1)
Bsp1286I 667 GAGCAC G[AGT]GC[ACT]^C
BspHI 264 TCATGA T^CATGA
BsrBI 160 GAGCGG CCGCTC(-3/-3)
BsrDI 200 CATTGC GCAATG(2/0)
BsrFI 13 GCCGGC [GA]^CCGG[CT]
BssHII 3 GCGCGC G^CGCGC
BssSI 881 CACGAG CACGAG(-5/-1)
Cfr10I 13 GCCGGC [GA]^CCGG[CT]
DsaI 84 CCGCGG C^C[GA][CT]GG
EcoRI 397 GAATTC G^AATTC
FauI 162 GCGGG CCCGC(4/6)
HindIII 361 AAGCTT A^AGCTT
HphI 367 GGTGA GGTGA(8/7)
KspI 84 CCGCGG CCGC^GG
MaeIII 981 GTCAC ^GT[ACGT]AC
MspA1I 84 CCGCGG C[AC]G^C[GT]G
MwoI 1003 GCTAATAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 13 GCCGGC GCC^GGC
NciI 60 CCCGG CC^[GC]GG
NgoMIV 13 GCCGGC G^CCGGC
NspI 1194 GCATGC [GA]CATG^[CT]
PleI 1102 GAGTC GAGTC(4/5)
RcaI 264 TCATGA T^CATGA
SacII 84 CCGCGG CCGC^GG
SpeI 984 ACTAGT A^CTAGT
SphI 1194 GCATGC GCATG^C
StuI 45 AGGCCT AGG^CCT
SwaI 515 ATTTAAAT ATTT^AAAT
Tsp45I 981 GTCAC ^GT[GC]AC
XmnI 776 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 4 CGCG CG^CG
85 CGCG
AlwI 743 GATCC GGATC(4/5)
957 GGATC
AlwNI 415 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
1213 CAGTTGCTG
BfaI 977 CTAG C^TAG
985 CTAG
BsaJI 24 CCTCGG C^C[ACGT][ACGT]GG
84 CCGCGG
BsiYI 19 CCTCGCCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
702 CCGAGTTAAGG
BslI 19 CCTCGCCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
702 CCGAGTTAAGG
BspMI 132 GCAGGT ACCTGC(4/8)
295 GCAGGT
BstNI 48 CCTGG CC^[AT]GG
1230 CCTGG
BstUI 4 CGCG CG^CG
85 CGCG
EcoRII 48 CCTGG ^CC[AT]GG
1230 CCTGG
EcoT22I 463 ATGCAT ATGCA^T
713 ATGCAT
HapII 14 CCGG C^CGG
61 CCGG
HpaII 14 CCGG C^CGG
61 CCGG
MaeI 977 CTAG C^TAG
985 CTAG
MslI 402 CACAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
748 CACAAAAGTG
MspI 14 CCGG C^CGG
61 CCGG
MvaI 48 CCTGG CC^[AT]GG
1230 CCTGG
MvnI 4 CGCG CG^CG
85 CGCG
NsiI 463 ATGCAT ATGCA^T
713 ATGCAT
SmlI 933 CTTAAG C^T[CT][GA]AG
944 CTCAAG
TaqI 857 TCGA T^CGA
969 TCGA
TthHB8I 857 TCGA T^CGA
969 TCGA
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcsI AluI ApoI BbsI
BbvI BpuAI BsrI BssKI BstF5I
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI Fnu4HI FokI
HaeIII HhaI HinP1I HinfI ItaI
Ksp632I MboI MboII MflI MnlI
MseI NdeII NlaIII PstI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
TfiI Tru9I TseI Tsp509I TspRI
XhoII