Restriction map for
pF1KB6637
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AviII AvrII BalI BanII
BbeI BcgI BciVI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaHI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1286I Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraIII DrdI DsaI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoRI EcoT22I FauI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MfeI
MluI MluNI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 333 TCCGGA T^CCGGA
AcsI 312 GAATTT [GA]^AATT[CT]
AhdI 910 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 853 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 312 GAATTT [GA]^AATT[CT]
AspEI 910 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1156 CCCGAG C^[CT]CG[GA]G
BamHI 75 GGATCC G^GATCC
BbrPI 697 CACGTG CAC^GTG
BclI 120 TGATCA T^GATCA
BcnI 1028 CCGGG CC^[GC]GG
BfaI 448 CTAG C^TAG
BsaBI 121 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 657 GAGACC GGTCTC(1/5)
BsaWI 333 TCCGGA [AT]^CCGG[AT]
BseAI 333 TCCGGA T^CCGGA
BsoBI 1156 CCCGAG C^[CT]CG[GA]G
BspEI 333 TCCGGA T^CCGGA
BsrFI 412 ACCGGC [GA]^CCGG[CT]
BssSI 15 CTCGTG CACGAG(-5/-1)
BstEII 708 GGTGACC G^GT[ACGT]ACC
BstPI 708 GGTGACC G^GT[ACGT]ACC
BstXI 890 CCAAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 412 ACCGGC [GA]^CCGG[CT]
DraI 640 TTTAAA TTT^AAA
Eam1105I 910 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 708 GGTGACC G^GT[ACGT]ACC
EcoRV 880 GATATC GAT^ATC
FbaI 120 TGATCA T^GATCA
HgaI 974 GCGTC GACGC(5/10)
MaeI 448 CTAG C^TAG
MamI 121 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 333 TCCGGA T^CCGGA
MslI 1137 CATACCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 1028 CCGGG CC^[GC]GG
NspI 746 GCATGT [GA]CATG^[CT]
PmaCI 697 CACGTG CAC^GTG
PmlI 697 CACGTG CAC^GTG
SapI 969 GAAGAGC GCTCTTC(1/4)
SexAI 704 ACCAGGT A^CC[AT]GGT
SmlI 566 CTCAAG C^T[CT][GA]AG
StuI 39 AGGCCT AGG^CCT
TaqI 1091 TCGA T^CGA
TthHB8I 1091 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 186 CCGC CCGC(-3/-1)
818 CCGC
BanI 723 GGTGCC G^G[CT][GA]CC
1044 GGCACC
BbvCI 429 CCTCAGC CCTCAGC(-5/-2)
70 GCTGAGG
BpmI 400 CTCCAG CTGGAG(16/14)
301 CTGGAG
BsaAI 550 TACGTG [CT]AC^GT[GA]
697 CACGTG
BseRI 367 CTCCTC GAGGAG(10/8)
1039 GAGGAG
BstYI 75 GGATCC [GA]^GATC[CT]
344 AGATCC
DraII 305 AGGGCCT [GA]G^G[ACGT]CC[CT]
437 AGGGCCT
EcoNI 66 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
429 CCTCAGCGAGG
EcoO109I 305 AGGGCCT [GA]G^G[ACGT]CC[CT]
437 AGGGCCT
EcoT14I 275 CCAAGG C^C[AT][AT]GG
1061 CCAAGG
HincII 505 GTCAAC GT[CT]^[GA]AC
983 GTCAAC
HindII 505 GTCAAC GT[CT]^[GA]AC
983 GTCAAC
HinfI 330 GATTC G^A[ACGT]TC
1239 GAATC
MaeII 551 ACGT A^CGT
698 ACGT
MaeIII 709 GTGAC ^GT[ACGT]AC
1033 GTCAC
MflI 75 GGATCC [GA]^GATC[CT]
344 AGATCC
SfcI 245 CTACAG C^T[GA][CT]AG
850 CTACAG
StyI 275 CCAAGG C^C[AT][AT]GG
1061 CCAAGG
TfiI 330 GATTC G^A[AT]TC
1239 GAATC
Tsp45I 709 GTGAC ^GT[GC]AC
1033 GTCAC
XhoII 75 GGATCC [GA]^GATC[CT]
344 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AfaI AluI AlwI AvaII BbsI
BbvI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI BsrDI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
Ksp632I MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI Tru9I TseI
Tsp509I TspRI