Restriction map for
pF1KB6639
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AflIII AhdI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I BspEI BspHI BspLU11I
BspMI BsrBI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI Bsu36I CelII CpoI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HgaI
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MroI MunI MvnI NarI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AcsI 721 GAATTT [GA]^AATT[CT]
AgeI 490 ACCGGT A^CCGGT
AlwI 1141 GGATC GGATC(4/5)
Aor51HI 686 AGCGCT AGC^GCT
ApoI 721 GAATTT [GA]^AATT[CT]
Asp700I 904 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 226 TGGCCA TGG^CCA
BbsI 316 GAAGAC GAAGAC(2/6)
BclI 132 TGATCA T^GATCA
BcnI 1034 CCCGG CC^[GC]GG
BpuAI 316 GAAGAC GAAGAC(2/6)
BsaI 626 GAGACC GGTCTC(1/5)
BsaWI 490 ACCGGT [AT]^CCGG[AT]
BsgI 427 GTGCAG GTGCAG(16/14)
BsmAI 626 GAGAC GTCTC(1/5)
BspDI 805 ATCGAT AT^CGAT
BsrDI 420 CATTGC GCAATG(2/0)
BstNI 409 CCAGG CC^[AT]GG
CfoI 687 GCGC GCG^C
ClaI 805 ATCGAT AT^CGAT
DraI 292 TTTAAA TTT^AAA
DraII 831 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 237 CCATGG C^C[GA][CT]GG
EaeI 226 TGGCCA [CT]^GGCC[GA]
Eco47III 686 AGCGCT AGC^GCT
EcoO109I 831 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 409 CCAGG ^CC[AT]GG
FauI 4 GCGGG CCCGC(4/6)
FbaI 132 TGATCA T^GATCA
HaeII 686 AGCGCT [GA]GCGC^[CT]
HhaI 687 GCGC GCG^C
HinP1I 687 GCGC G^CGC
HincII 486 GTTGAC GT[CT]^[GA]AC
HindII 486 GTTGAC GT[CT]^[GA]AC
MluNI 226 TGGCCA TGG^CCA
MscI 226 TGGCCA TGG^CCA
MspA1I 561 CAGCTG C[AC]G^C[GT]G
MvaI 409 CCAGG CC^[AT]GG
NaeI 15 GCCGGC GCC^GGC
NciI 1034 CCCGG CC^[GC]GG
NcoI 237 CCATGG C^CATGG
NdeI 212 CATATG CA^TATG
NgoMIV 15 GCCGGC G^CCGGC
NlaIV 831 GGGACC GG[ACGT]^[ACGT]CC
NspI 1112 ACATGC [GA]CATG^[CT]
PflMI 1234 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 490 ACCGGT A^CCGGT
PpuMI 831 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 561 CAGCTG CAG^CTG
SmlI 462 CTCAAG C^T[CT][GA]AG
TaqI 806 TCGA T^CGA
TthHB8I 806 TCGA T^CGA
Van91I 1234 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 904 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 935 GACGTC GACGT^C
995 GACGTC
AccI 45 GTATAC GT^[AC][GT]AC
765 GTATAC
AciI 234 CCGC CCGC(-3/-1)
4 GCGG
AcyI 935 GACGTC G[GA]^CG[CT]C
995 GACGTC
AlwNI 308 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
613 CAGCAACTG
BsaHI 935 GACGTC G[GA]^CG[CT]C
995 GACGTC
BsaJI 237 CCATGG C^C[ACGT][ACGT]GG
659 CCTTGG
Bsp1407I 416 TGTACA T^GTACA
1092 TGTACA
BsrFI 15 GCCGGC [GA]^CCGG[CT]
490 ACCGGT
BsrGI 416 TGTACA T^GTACA
1092 TGTACA
BsrI 769 ACTGG ACTGG(1/-1)
1234 CCAGT
BssKI 409 CCAGG ^CC[ACGT]GG
1034 CCCGG
Bst1107I 45 GTATAC GTA^TAC
765 GTATAC
BstF5I 90 CATCC GGATG(2/0)
850 CATCC
BstZ17I 45 GTATAC GTA^TAC
765 GTATAC
Cfr10I 15 GCCGGC [GA]^CCGG[CT]
490 ACCGGT
EcoT14I 237 CCATGG C^C[AT][AT]GG
659 CCTTGG
FokI 90 CATCC GGATG(9/13)
850 CATCC
HaeIII 18 GGCC GG^CC
227 GGCC
Hin1I 935 GACGTC G[GA]^CG[CT]C
995 GACGTC
MaeIII 520 GTCAC ^GT[ACGT]AC
1226 GTCAC
MslI 420 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
531 CATTCCTGTG
ScrFI 409 CCAGG CC^[ACGT]GG
1034 CCCGG
SfaNI 1030 GATGC GCATC(5/9)
451 GCATC
SfcI 690 CTATAG C^T[GA][CT]AG
1025 CTGTAG
SspBI 416 TGTACA T^GTACA
1092 TGTACA
SspI 296 AATATT AAT^ATT
952 AATATT
StyI 237 CCATGG C^C[AT][AT]GG
659 CCTTGG
TfiI 641 GAATC G^A[AT]TC
773 GAATC
Tsp45I 520 GTCAC ^GT[GC]AC
1226 GTCAC
TspRI 507 CAGTG CA[GC]TG[ACGT][ACGT]^
1235 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AfaI AluI AvaII BbvI BfaI
BpmI BsiYI BslI BsmFI Cac8I
Cfr13I DdeI DpnII Fnu4HI HapII
HinfI HpaII HphI ItaI MaeI
MaeII MboI MboII MnlI MseI
MspI MwoI NdeII NlaIII PleI
RsaI Sau3AI Sau96I Tru9I TseI
Tsp509I