Restriction map for
pF1KB6643
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AluI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspHI
AviII AvrII BanI BbeI BbrPI
BbvCI BcgI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstBI Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DrdI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoRI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaII HphI
KasI KpnI Ksp632I KspI MaeI
MaeII MfeI MluI MroI MspI
MunI NaeI NarI NciI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SwaI TaqI Tsp45I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 175 GTATAC GT^[AC][GT]AC
AccII 15 CGCG CG^CG
AcsI 882 AAATTC [GA]^AATT[CT]
AhdI 537 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 774 GGGCCC GGGCC^C
ApoI 882 AAATTC [GA]^AATT[CT]
AspEI 537 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 21 CCCGAG C^[CT]CG[GA]G
BalI 894 TGGCCA TGG^CCA
BamHI 472 GGATCC G^GATCC
BanII 774 GGGCCC G[GA]GC[CT]^C
BbsI 802 GAAGAC GAAGAC(2/6)
BciVI 1032 GTATCC GTATCC(6/5)
BmyI 774 GGGCCC G[AGT]GC[ACT]^C
BpuAI 802 GAAGAC GAAGAC(2/6)
BsaBI 196 GATTCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 764 CTCCTC GAGGAG(10/8)
BsgI 344 CTGCAC GTGCAG(16/14)
BsoBI 21 CCCGAG C^[CT]CG[GA]G
Bsp1286I 774 GGGCCC G[AGT]GC[ACT]^C
BsrBI 276 GAGCGG CCGCTC(-3/-3)
BsrI 520 CCAGT ACTGG(1/-1)
Bst1107I 175 GTATAC GTA^TAC
BstAPI 153 GCAGATGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 976 GGTTACC G^GT[ACGT]ACC
BstPI 976 GGTTACC G^GT[ACGT]ACC
BstUI 15 CGCG CG^CG
BstXI 375 CCAGATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 472 GGATCC [GA]^GATC[CT]
BstZ17I 175 GTATAC GTA^TAC
Cac8I 515 GCATGC GC[ACGT]^[ACGT]GC
DraIII 1010 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 537 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 844 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 976 GGTTACC G^GT[ACGT]ACC
EcoRV 938 GATATC GAT^ATC
FauI 52 CCCGC CCCGC(4/6)
HincII 712 GTTAAC GT[CT]^[GA]AC
HindII 712 GTTAAC GT[CT]^[GA]AC
HpaI 712 GTTAAC GTT^AAC
MamI 196 GATTCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 472 GGATCC [GA]^GATC[CT]
MluNI 894 TGGCCA TGG^CCA
MscI 894 TGGCCA TGG^CCA
MseI 713 TTAA T^TAA
MslI 447 CATGAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 53 CCGCTG C[AC]G^C[GT]G
MvnI 15 CGCG CG^CG
ScaI 788 AGTACT AGT^ACT
SfcI 756 CTATAG C^T[GA][CT]AG
SphI 515 GCATGC GCATG^C
SspI 866 AATATT AAT^ATT
StuI 1117 AGGCCT AGG^CCT
Tru9I 713 TTAA T^TAA
TspRI 97 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 472 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 473 GATCC GGATC(4/5)
472 GGATC
AspI 836 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1052 GACAAGGTC
BpmI 845 CTCCAG CTGGAG(16/14)
260 CTGGAG
BsmAI 741 GAGAC GTCTC(1/5)
216 GTCTC
BstF5I 75 GGATG GGATG(2/0)
266 GGATG
DpnII 45 GATC ^GATC
473 GATC
DsaI 468 CCATGG C^C[GA][CT]GG
476 CCATGG
EaeI 372 CGGCCA [CT]^GGCC[GA]
894 TGGCCA
EcoT22I 616 ATGCAT ATGCA^T
908 ATGCAT
FokI 75 GGATG GGATG(9/13)
266 GGATG
MaeIII 485 GTTAC ^GT[ACGT]AC
977 GTTAC
MboI 45 GATC ^GATC
473 GATC
MboII 66 GAAGA GAAGA(8/7)
802 GAAGA
NcoI 468 CCATGG C^CATGG
476 CCATGG
NdeI 394 CATATG CA^TATG
727 CATATG
NdeII 45 GATC ^GATC
473 GATC
NsiI 616 ATGCAT ATGCA^T
908 ATGCAT
PflFI 836 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1052 GACAAGGTC
PleI 109 GACTC GAGTC(4/5)
543 GAGTC
Sau3AI 45 GATC ^GATC
473 GATC
Tsp509I 883 AATT ^AATT
1150 AATT
Tth111I 836 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1052 GACAAGGTC
XcmI 819 CCAATACAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1134 CCAGGGACAGTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AfaI AvaII BbvI BsaJI
BsiYI BslI BsmFI BssKI BstNI
Cfr13I DdeI DraII EcoO109I EcoRII
EcoT14I Fnu4HI HaeIII HinfI ItaI
MnlI MvaI MwoI NlaIII NlaIV
NspI PpuMI RsaI Sau96I ScrFI
SfaNI StyI TfiI TseI