Restriction map for
pF1KB6669
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AocI
ApaI AscI AseI AsnI Asp718I
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DrdI DsaI EaeI EagI EclXI
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AflIII 412 ACATGT A^C[GA][CT]GT
AhdI 408 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1233 GTGCAC G^TGCAC
AlwI 1036 GATCC GGATC(4/5)
AlwNI 860 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1233 GTGCAC G^TGCAC
Asp700I 796 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 408 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 39 CTCGGG C^[CT]CG[GA]G
AvaII 482 GGTCC G^G[AT]CC
BanI 213 GGTGCC G^G[CT][GA]CC
BcgI 1125 CGATGCAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 775 GCCGTTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 770 CTCCAG CTGGAG(16/14)
BsaAI 939 TACGTG [CT]AC^GT[GA]
BsaI 1185 GGTCTC GGTCTC(1/5)
BseRI 235 GAGGAG GAGGAG(10/8)
BsmFI 416 GTCCC GGGAC(10/14)
BsmI 1023 GAATGC GAATGC(1/-1)
BsoBI 39 CTCGGG C^[CT]CG[GA]G
Bsp1407I 105 TGTACA T^GTACA
BspLU11I 412 ACATGT A^CATGT
BsrDI 551 GCAATG GCAATG(2/0)
BsrGI 105 TGTACA T^GTACA
Cac8I 526 GCCTGC GC[ACGT]^[ACGT]GC
DraI 319 TTTAAA TTT^AAA
DraII 481 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 224 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 408 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 608 CTCTTC CTCTTC(1/4)
EcoNI 910 CCTGAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 481 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1077 GAATTC G^AATTC
EcoT14I 980 CCTTGG C^C[AT][AT]GG
FauI 879 GCGGG CCCGC(4/6)
HindIII 1192 AAGCTT A^AGCTT
Ksp632I 608 CTCTTC CTCTTC(1/4)
MslI 864 CACTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 412 ACATGT [GA]CATG^[CT]
PpuMI 481 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 475 CTGCAG CTGCA^G
PvuII 667 CAGCTG CAG^CTG
SacI 975 GAGCTC GAGCT^C
SmlI 724 CTTGAG C^T[CT][GA]AG
SspBI 105 TGTACA T^GTACA
SspI 653 AATATT AAT^ATT
StyI 980 CCTTGG C^C[AT][AT]GG
TaqI 587 TCGA T^CGA
TthHB8I 587 TCGA T^CGA
XmnI 796 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 315 AAATTT [GA]^AATT[CT]
1077 GAATTC
AfaI 106 GTAC GT^AC
674 GTAC
Aor51HI 171 AGCGCT AGC^GCT
1065 AGCGCT
ApoI 315 AAATTT [GA]^AATT[CT]
1077 GAATTC
BanII 919 GGGCTC G[GA]GC[CT]^C
975 GAGCTC
BcnI 756 CCGGG CC^[GC]GG
1182 CCCGG
BglII 35 AGATCT A^GATCT
602 AGATCT
BsgI 1209 CTGCAC GTGCAG(16/14)
1061 GTGCAG
BstYI 35 AGATCT [GA]^GATC[CT]
602 AGATCT
CfoI 172 GCGC GCG^C
1066 GCGC
Eco47III 171 AGCGCT AGC^GCT
1065 AGCGCT
HaeII 171 AGCGCT [GA]GCGC^[CT]
1065 AGCGCT
HapII 756 CCGG C^CGG
1183 CCGG
HhaI 172 GCGC GCG^C
1066 GCGC
HinP1I 172 GCGC G^CGC
1066 GCGC
HinfI 535 GAATC G^A[ACGT]TC
647 GAATC
HpaII 756 CCGG C^CGG
1183 CCGG
MaeII 940 ACGT A^CGT
1231 ACGT
MflI 35 AGATCT [GA]^GATC[CT]
602 AGATCT
MspA1I 667 CAGCTG C[AC]G^C[GT]G
877 CAGCGG
MspI 756 CCGG C^CGG
1183 CCGG
NciI 756 CCGGG CC^[GC]GG
1182 CCCGG
NlaIV 213 GGTGCC GG[ACGT]^[ACGT]CC
920 GGCTCC
RsaI 106 GTAC GT^AC
674 GTAC
SfcI 475 CTGCAG C^T[GA][CT]AG
486 CTACAG
TfiI 535 GAATC G^A[AT]TC
647 GAATC
XhoII 35 AGATCT [GA]^GATC[CT]
602 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AspHI BbvI BmyI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HphI ItaI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII Sau3AI Sau96I
ScrFI SfaNI Tru9I TseI Tsp45I
Tsp509I TspRI