Restriction map for
pF1KB6672
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BclI
BglI BlnI BlpI Bpu1102I BsaHI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MfeI MluI
MluNI MscI MunI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccIII 593 TCCGGA T^CCGGA
AciI 975 CCGC CCGC(-3/-1)
AflII 22 CTTAAG C^TTAAG
AseI 781 ATTAAT AT^TAAT
AsnI 781 ATTAAT AT^TAAT
AspHI 1256 GTGCTC G[AT]GC[AT]^C
AspI 463 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcnI 1086 CCGGG CC^[GC]GG
BfrI 22 CTTAAG C^TTAAG
BglII 690 AGATCT A^GATCT
BmyI 1256 GTGCTC G[AGT]GC[ACT]^C
BsaAI 1237 CACGTA [CT]AC^GT[GA]
BsaBI 685 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 322 GAGACC GGTCTC(1/5)
BsaJI 311 CCTTGG C^C[ACGT][ACGT]GG
BseAI 593 TCCGGA T^CCGGA
BsiHKAI 1256 GTGCTC G[AT]GC[AT]^C
BsiYI 1086 CCGGGATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1086 CCGGGATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 876 GGGAC GGGAC(10/14)
BsmI 1182 GAATGC GAATGC(1/-1)
Bsp1286I 1256 GTGCTC G[AGT]GC[ACT]^C
BspEI 593 TCCGGA T^CCGGA
BspHI 470 TCATGA T^CATGA
BstNI 208 CCTGG CC^[AT]GG
Cac8I 427 GCCAGC GC[ACGT]^[ACGT]GC
DraII 876 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 876 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 589 GAATTC G^AATTC
EcoRII 208 CCTGG ^CC[AT]GG
EcoRV 751 GATATC GAT^ATC
EcoT14I 311 CCTTGG C^C[AT][AT]GG
EcoT22I 1 ATGCAT ATGCA^T
HincII 481 GTCAAC GT[CT]^[GA]AC
HindII 481 GTCAAC GT[CT]^[GA]AC
HindIII 722 AAGCTT A^AGCTT
MaeII 1238 ACGT A^CGT
MamI 685 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 593 TCCGGA T^CCGGA
MspA1I 584 CAGCTG C[AC]G^C[GT]G
MvaI 208 CCTGG CC^[AT]GG
NciI 1086 CCGGG CC^[GC]GG
NdeI 1177 CATATG CA^TATG
NsiI 1 ATGCAT ATGCA^T
PflFI 463 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 876 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 878 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 781 ATTAAT AT^TAAT
PvuII 584 CAGCTG CAG^CTG
RcaI 470 TCATGA T^CATGA
ScaI 293 AGTACT AGT^ACT
SfcI 1199 CTATAG C^T[GA][CT]AG
SmlI 22 CTTAAG C^T[CT][GA]AG
StyI 311 CCTTGG C^C[AT][AT]GG
Tth111I 463 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 555 CCAAATTCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 581 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1207 CAGACTCTG
AvaII 606 GGACC G^G[AT]CC
877 GGACC
BbsI 122 GAAGAC GAAGAC(2/6)
869 GAAGAC
BfaI 61 CTAG C^TAG
424 CTAG
BpuAI 122 GAAGAC GAAGAC(2/6)
869 GAAGAC
BsaWI 593 TCCGGA [AT]^CCGG[AT]
608 ACCGGA
BsgI 227 CTGCAC GTGCAG(16/14)
815 GTGCAG
BssKI 208 CCTGG ^CC[ACGT]GG
1086 CCGGG
EarI 283 GAAGAG CTCTTC(1/4)
319 GAAGAG
HaeIII 717 GGCC GG^CC
906 GGCC
HgaI 833 GACGC GACGC(5/10)
220 GCGTC
Ksp632I 283 GAAGAG CTCTTC(1/4)
319 GAAGAG
MaeI 61 CTAG C^TAG
424 CTAG
MaeIII 577 GTGAC ^GT[ACGT]AC
760 GTGAC
MslI 465 CATGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1177 CATATGAATG
NlaIV 131 GGAACC GG[ACGT]^[ACGT]CC
876 GGGACC
ScrFI 208 CCTGG CC^[ACGT]GG
1086 CCGGG
TaqI 540 TCGA T^CGA
936 TCGA
Tsp45I 577 GTGAC ^GT[GC]AC
760 GTGAC
TthHB8I 540 TCGA T^CGA
936 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BciVI BpmI BseRI BsmAI
BsrI BstF5I BstYI Cfr13I DdeI
DpnII Fnu4HI FokI HapII HinfI
HpaII HphI ItaI MboI MboII
MflI MnlI MseI MspI MwoI
NdeII NlaIII PleI RsaI Sau3AI
Sau96I SfaNI Tru9I TseI Tsp509I
TspRI XhoII