Restriction map for
pF1KB6676
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BciVI BclI BfrI BglII
BlnI BsaAI BsaI BsgI BsiEI
BsiWI BsmI BspHI BspLU11I BspMI
BsrDI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FbaI FseI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MaeIII MfeI MluI MluNI MscI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI Tsp45I Van91I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 408 TCCGGA T^CCGGA
AcyI 1201 GACGCC G[GA]^CG[CT]C
Asp700I 1075 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 936 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 784 CTCGGG C^[CT]CG[GA]G
BcgI 1188 CGATGCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 256 CTAG C^TAG
BglI 1240 GCCACCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 54 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 54 GCTCAGC GC^T[ACGT]AGC
BsaBI 34 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1201 GACGCC G[GA]^CG[CT]C
BseAI 408 TCCGGA T^CCGGA
BseRI 982 GAGGAG GAGGAG(10/8)
BsmAI 735 GAGAC GTCTC(1/5)
BsmBI 735 GAGACG CGTCTC(1/5)
BsmFI 150 GTCCC GGGAC(10/14)
BsoBI 784 CTCGGG C^[CT]CG[GA]G
Bsp1407I 26 TGTACA T^GTACA
BspDI 1186 ATCGAT AT^CGAT
BspEI 408 TCCGGA T^CCGGA
BsrBI 1001 GAGCGG CCGCTC(-3/-3)
BsrGI 26 TGTACA T^GTACA
BssSI 758 CACGAG CACGAG(-5/-1)
CelII 54 GCTCAGC GC^T[ACGT]AGC
ClaI 1186 ATCGAT AT^CGAT
DraI 976 TTTAAA TTT^AAA
DsaI 1044 CCATGG C^C[GA][CT]GG
EcoNI 1290 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 581 ATGCAT ATGCA^T
FauI 1003 GCGGG CCCGC(4/6)
Hin1I 1201 GACGCC G[GA]^CG[CT]C
MaeI 256 CTAG C^TAG
MamI 34 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 408 TCCGGA T^CCGGA
MseI 977 TTAA T^TAA
MslI 1089 CACAGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1044 CCATGG C^CATGG
NdeI 578 CATATG CA^TATG
NsiI 581 ATGCAT ATGCA^T
PflFI 936 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 331 GAAGAGC GCTCTTC(1/4)
SmlI 1060 CTCAAG C^T[CT][GA]AG
SspBI 26 TGTACA T^GTACA
SspI 535 AATATT AAT^ATT
Tru9I 977 TTAA T^TAA
Tth111I 936 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 255 TCTAGA T^CTAGA
XcmI 1280 CCATCACTTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1075 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 358 GAATTC [GA]^AATT[CT]
1049 GAATTC
AfaI 27 GTAC GT^AC
390 GTAC
AlwNI 10 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
73 CAGATCCTG
ApaI 302 GGGCCC GGGCC^C
788 GGGCCC
ApoI 358 GAATTC [GA]^AATT[CT]
1049 GAATTC
AvaII 1224 GGACC G^G[AT]CC
1329 GGACC
BbsI 682 GAAGAC GAAGAC(2/6)
763 GAAGAC
BcnI 305 CCCGG CC^[GC]GG
1099 CCCGG
BpuAI 682 GAAGAC GAAGAC(2/6)
763 GAAGAC
BsaWI 408 TCCGGA [AT]^CCGG[AT]
968 TCCGGT
BsiYI 1215 CCTGGGCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1290 CCTGGGGAAGG
BslI 1215 CCTGGGCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1290 CCTGGGGAAGG
BstF5I 966 CATCC GGATG(2/0)
1113 CATCC
DraII 301 AGGGCCC [GA]G^G[ACGT]CC[CT]
787 GGGGCCC
EcoO109I 301 AGGGCCC [GA]G^G[ACGT]CC[CT]
787 GGGGCCC
EcoRI 358 GAATTC G^AATTC
1049 GAATTC
FokI 966 CATCC GGATG(9/13)
1113 CATCC
HgaI 737 GACGC GACGC(5/10)
1201 GACGC
MspA1I 722 CAGCTG C[AC]G^C[GT]G
1333 CAGCTG
NciI 305 CCCGG CC^[GC]GG
1099 CCCGG
NspI 1094 GCATGC [GA]CATG^[CT]
1236 GCATGC
PstI 400 CTGCAG CTGCA^G
1196 CTGCAG
PvuII 722 CAGCTG CAG^CTG
1333 CAGCTG
RsaI 27 GTAC GT^AC
390 GTAC
SacI 52 GAGCTC GAGCT^C
1300 GAGCTC
SfaNI 1189 GATGC GCATC(5/9)
700 GCATC
SphI 1094 GCATGC GCATG^C
1236 GCATGC
TfiI 556 GATTC G^A[AT]TC
601 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwI AspHI BanII
BbvI BmyI BpmI BsaJI BsiHKAI
Bsp1286I BsrI BssKI BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MboI MboII MflI MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfcI
StyI TaqI TseI Tsp509I TspRI
TthHB8I XhoII