Restriction map for
pF1KB6537
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsiEI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DpnII DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoO109I EcoO65I EcoT22I
FbaI FseI FspI HindIII HinfI
HpaI KpnI KspI MaeI MamI
MboI MfeI MflI MluI MluNI
MscI MseI MspA1I MunI NaeI
NcoI NdeII NgoMIV NheI NotI
NsiI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI TfiI Tru9I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 976 CGCG CG^CG
AccIII 882 TCCGGA T^CCGGA
AcyI 51 GGCGCC G[GA]^CG[CT]C
AfaI 318 GTAC GT^AC
AspI 400 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 51 GGCGCC G^G[CT][GA]CC
BbeI 51 GGCGCC GGCGC^C
BbsI 1096 GAAGAC GAAGAC(2/6)
BcnI 55 CCCGG CC^[GC]GG
BpmI 1053 CTCCAG CTGGAG(16/14)
BpuAI 1096 GAAGAC GAAGAC(2/6)
BsaHI 51 GGCGCC G[GA]^CG[CT]C
BseAI 882 TCCGGA T^CCGGA
BsmFI 843 GTCCC GGGAC(10/14)
BsmI 441 GAATGC GAATGC(1/-1)
BspEI 882 TCCGGA T^CCGGA
BspMI 659 ACCTGC ACCTGC(4/8)
BstUI 976 CGCG CG^CG
EaeI 473 TGGCCG [CT]^GGCC[GA]
EarI 36 GAAGAG CTCTTC(1/4)
EcoNI 173 CCTATGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 850 GAATTC G^AATTC
EcoRV 1078 GATATC GAT^ATC
EcoT14I 20 CCAAGG C^C[AT][AT]GG
FauI 630 GCGGG CCCGC(4/6)
Hin1I 51 GGCGCC G[GA]^CG[CT]C
KasI 51 GGCGCC G^GCGCC
Ksp632I 36 GAAGAG CTCTTC(1/4)
MroI 882 TCCGGA T^CCGGA
MslI 372 CACGGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 976 CGCG CG^CG
NarI 51 GGCGCC GG^CGCC
NciI 55 CCCGG CC^[GC]GG
NdeI 901 CATATG CA^TATG
NruI 975 TCGCGA TCG^CGA
NspI 566 GCATGT [GA]CATG^[CT]
PflFI 400 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 318 GTAC GT^AC
SfoI 51 GGCGCC GGC^GCC
SnaBI 315 TACGTA TAC^GTA
SspI 249 AATATT AAT^ATT
StyI 20 CCAAGG C^C[AT][AT]GG
Tsp45I 272 GTCAC ^GT[GC]AC
Tth111I 400 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 784 GAATTT [GA]^AATT[CT]
850 GAATTC
ApoI 784 GAATTT [GA]^AATT[CT]
850 GAATTC
Asp700I 300 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
784 GAATTTGTTC
AvaII 58 GGACC G^G[AT]CC
880 GGTCC
BsaAI 315 TACGTA [CT]AC^GT[GA]
421 TACGTG
BsaJI 20 CCAAGG C^C[ACGT][ACGT]GG
448 CCCTGG
BsaWI 482 ACCGGA [AT]^CCGG[AT]
882 TCCGGA
BseRI 39 GAGGAG GAGGAG(10/8)
835 GAGGAG
BsgI 100 GTGCAG GTGCAG(16/14)
110 GTGCAG
HgaI 400 GACGC GACGC(5/10)
453 GACGC
HincII 157 GTTGAC GT[CT]^[GA]AC
736 GTTGAC
HindII 157 GTTGAC GT[CT]^[GA]AC
736 GTTGAC
MaeII 316 ACGT A^CGT
422 ACGT
MaeIII 184 GTAAC ^GT[ACGT]AC
272 GTCAC
NlaIII 502 CATG CATG^
567 CATG
XmnI 300 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
784 GAATTTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI Aor51HI AspHI AvaI
BanII BbvI BmyI BsiHKAI BsiYI
BslI BsoBI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I Eco47III
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboII MnlI MspI MvaI
MwoI NlaIV PstI SacI Sau96I
ScrFI SfaNI SfcI TseI Tsp509I
TspRI