Restriction map for
pF1KB6553
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HincII HindII HpaI KpnI KspI
MamI MfeI MluI MroI MseI
MunI NdeI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI Tru9I Tsp509I
Tth111I TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 882 CGCG CG^CG
AfaI 441 GTAC GT^AC
AlwNI 788 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 676 GTGCTC G[AT]GC[AT]^C
BbrPI 781 CACGTG CAC^GTG
BbsI 226 GTCTTC GAAGAC(2/6)
BpuAI 226 GTCTTC GAAGAC(2/6)
BsaAI 781 CACGTG [CT]AC^GT[GA]
BsiHKAI 676 GTGCTC G[AT]GC[AT]^C
BsrDI 161 GCAATG GCAATG(2/0)
BstUI 882 CGCG CG^CG
BstYI 968 GGATCT [GA]^GATC[CT]
EagI 472 CGGCCG C^GGCCG
EarI 523 CTCTTC CTCTTC(1/4)
EclXI 472 CGGCCG C^GGCCG
Eco52I 472 CGGCCG C^GGCCG
EcoT22I 974 ATGCAT ATGCA^T
HindIII 1011 AAGCTT A^AGCTT
Ksp632I 523 CTCTTC CTCTTC(1/4)
MflI 968 GGATCT [GA]^GATC[CT]
MslI 448 CATGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 882 CGCG CG^CG
NaeI 197 GCCGGC GCC^GGC
NcoI 447 CCATGG C^CATGG
NgoMIV 197 GCCGGC G^CCGGC
NheI 105 GCTAGC G^CTAGC
NsiI 974 ATGCAT ATGCA^T
NspI 365 GCATGT [GA]CATG^[CT]
PflMI 959 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1066 GAGTC GAGTC(4/5)
PmaCI 781 CACGTG CAC^GTG
PmlI 781 CACGTG CAC^GTG
PstI 352 CTGCAG CTGCA^G
RsaI 441 GTAC GT^AC
SapI 522 GCTCTTC GCTCTTC(1/4)
SfcI 352 CTGCAG C^T[GA][CT]AG
StuI 727 AGGCCT AGG^CCT
TfiI 693 GATTC G^A[AT]TC
Van91I 959 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 968 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 222 ACACGT A^C[GA][CT]GT
782 ACGTGT
AlwI 387 GATCC GGATC(4/5)
968 GGATC
BbeI 737 GGCGCC GGCGC^C
912 GGCGCC
BseRI 375 CTCCTC GAGGAG(10/8)
1086 CTCCTC
BsiEI 306 CGACCG CG[GA][CT]^CG
472 CGGCCG
BstF5I 37 CATCC GGATG(2/0)
812 GGATG
BstXI 395 CCATGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
447 CCATGGAAGTGG
DpnII 387 GATC ^GATC
969 GATC
EcoNI 901 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1092 CCTGGCCAAGG
EcoT14I 447 CCATGG C^C[AT][AT]GG
1097 CCAAGG
FokI 37 CATCC GGATG(9/13)
812 GGATG
HinfI 693 GATTC G^A[ACGT]TC
1066 GAGTC
HphI 769 GGTGA GGTGA(8/7)
617 TCACC
KasI 737 GGCGCC G^GCGCC
912 GGCGCC
MaeIII 565 GTCAC ^GT[ACGT]AC
616 GTCAC
MboI 387 GATC ^GATC
969 GATC
NarI 737 GGCGCC GG^CGCC
912 GGCGCC
NdeII 387 GATC ^GATC
969 GATC
Sau3AI 387 GATC ^GATC
969 GATC
SfoI 737 GGCGCC GGC^GCC
912 GGCGCC
StyI 447 CCATGG C^C[AT][AT]GG
1097 CCAAGG
Tsp45I 565 GTCAC ^GT[GC]AC
616 GTCAC
XcmI 134 CCATAGTCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1082 CCAGCTCCTCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcyI AluI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BglI BmyI BsaHI BsaJI
BsgI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI MaeI MaeII MboII MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PpuMI
PvuII Sau96I ScrFI SfaNI SfiI
SmaI TseI TspRI XmaCI XmaI