Restriction map for
pF1KB6566
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspHI AvrII BamHI
BbeI BbrPI BfrI BglII BlnI
BsaAI BsaI BseAI BsgI BsiHKAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI HincII HindII HindIII HpaI
KasI MfeI MluI MroI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NruI NsiI NspV
PacI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Van91I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 344 GGTACC G^GTACC
AcyI 199 GGCGTC G[GA]^CG[CT]C
AhdI 1102 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 364 GGGCCC GGGCC^C
Asp718I 344 GGTACC G^GTACC
AspEI 1102 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 207 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 961 GAAGAC GAAGAC(2/6)
BbvCI 672 GCTGAGG CCTCAGC(-5/-2)
BciVI 133 GTATCC GTATCC(6/5)
BclI 1022 TGATCA T^GATCA
BfaI 322 CTAG C^TAG
BlpI 939 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 939 GCTGAGC GC^T[ACGT]AGC
BpuAI 961 GAAGAC GAAGAC(2/6)
BsaBI 603 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 199 GGCGTC G[GA]^CG[CT]C
BsaWI 788 ACCGGA [AT]^CCGG[AT]
BsmAI 508 GAGAC GTCTC(1/5)
BsmBI 508 GAGACG CGTCTC(1/5)
BsmI 104 GCATTC GAATGC(1/-1)
CelII 939 GCTGAGC GC^T[ACGT]AGC
DsaI 126 CCGCGG C^C[GA][CT]GG
EagI 123 CGGCCG C^GGCCG
Eam1105I 1102 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 625 GAAGAG CTCTTC(1/4)
EclXI 123 CGGCCG C^GGCCG
Eco52I 123 CGGCCG C^GGCCG
FbaI 1022 TGATCA T^GATCA
HaeII 1017 GGCGCT [GA]GCGC^[CT]
Hin1I 199 GGCGTC G[GA]^CG[CT]C
KpnI 344 GGTACC GGTAC^C
Ksp632I 625 GAAGAG CTCTTC(1/4)
KspI 126 CCGCGG CCGC^GG
MaeI 322 CTAG C^TAG
MaeII 34 ACGT A^CGT
MamI 603 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 528 CATGAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NotI 122 GCGGCCGC GC^GGCCGC
NspI 752 ACATGC [GA]CATG^[CT]
PaeR7I 1150 CTCGAG C^TCGAG
PflFI 207 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 126 CCGCGG CCGC^GG
SapI 625 GAAGAGC GCTCTTC(1/4)
Tth111I 207 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 587 CCAGGACCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1150 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 333 GATCC GGATC(4/5)
741 GATCC
AvaI 171 CTCGGG C^[CT]CG[GA]G
1150 CTCGAG
AviII 524 TGCGCA TGC^GCA
557 TGCGCA
BalI 584 TGGCCA TGG^CCA
849 TGGCCA
BcgI 725 GCACCGATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1142 GCAGCCTGCTCG
BseRI 168 CTCCTC GAGGAG(10/8)
655 GAGGAG
BsiEI 123 CGGCCG CG[GA][CT]^CG
264 CGACCG
BsmFI 413 GGGAC GGGAC(10/14)
1155 GGGAC
BsoBI 171 CTCGGG C^[CT]CG[GA]G
1150 CTCGAG
BsrBI 716 CCGCTC CCGCTC(-3/-3)
1069 GAGCGG
BstYI 332 AGATCC [GA]^GATC[CT]
740 AGATCC
EcoNI 282 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1146 CCTGCTCGAGG
EcoT14I 956 CCTTGG C^C[AT][AT]GG
1190 CCAAGG
FspI 524 TGCGCA TGC^GCA
557 TGCGCA
HgaI 200 GCGTC GACGC(5/10)
211 GCGTC
HphI 327 GGTGA GGTGA(8/7)
812 TCACC
MflI 332 AGATCC [GA]^GATC[CT]
740 AGATCC
MluNI 584 TGGCCA TGG^CCA
849 TGGCCA
MscI 584 TGGCCA TGG^CCA
849 TGGCCA
PpuMI 404 AGGACCT [GA]G^G[AT]CC[CT]
1193 AGGTCCT
SfaNI 603 GATGC GCATC(5/9)
796 GATGC
SmlI 916 CTTGAG C^T[CT][GA]AG
1150 CTCGAG
StuI 48 AGGCCT AGG^CCT
615 AGGCCT
StyI 956 CCTTGG C^C[AT][AT]GG
1190 CCAAGG
Tsp45I 749 GTGAC ^GT[GC]AC
933 GTCAC
Tsp509I 830 AATT ^AATT
1177 AATT
TspRI 699 CAGTG CA[GC]TG[ACGT][ACGT]^
968 CACTG
XhoII 332 AGATCC [GA]^GATC[CT]
740 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaII BanI BanII BbvI BcnI
BglI BmyI BpmI BsaJI BsiYI
BslI Bsp1286I BspMI BsrI BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI TaqI TfiI
TseI TthHB8I