Restriction map for
pF1KB6578
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# NO CUT ENZYMES ( 134 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
AocI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HgaI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MluI MroI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 268 GACGTC GACGT^C
AcyI 268 GACGTC G[GA]^CG[CT]C
Aor51HI 800 AGCGCT AGC^GCT
BalI 305 TGGCCA TGG^CCA
BamHI 582 GGATCC G^GATCC
BanI 158 GGCACC G^G[CT][GA]CC
BbsI 976 GTCTTC GAAGAC(2/6)
BclI 990 TGATCA T^GATCA
BpmI 520 CTCCAG CTGGAG(16/14)
BpuAI 976 GTCTTC GAAGAC(2/6)
BsaHI 268 GACGTC G[GA]^CG[CT]C
BsaI 671 GGTCTC GGTCTC(1/5)
BsiEI 144 CGGTCG CG[GA][CT]^CG
BsmAI 672 GTCTC GTCTC(1/5)
BspDI 700 ATCGAT AT^CGAT
BsrBI 409 GAGCGG CCGCTC(-3/-3)
BssHII 183 GCGCGC G^CGCGC
BssSI 1024 CACGAG CACGAG(-5/-1)
BstAPI 938 GCAGTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 582 GGATCC [GA]^GATC[CT]
ClaI 700 ATCGAT AT^CGAT
Eco47III 800 AGCGCT AGC^GCT
FbaI 990 TGATCA T^GATCA
Hin1I 268 GACGTC G[GA]^CG[CT]C
MaeII 269 ACGT A^CGT
MfeI 730 CAATTG C^AATTG
MflI 582 GGATCC [GA]^GATC[CT]
MluNI 305 TGGCCA TGG^CCA
MscI 305 TGGCCA TGG^CCA
MslI 508 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 730 CAATTG C^AATTG
NspI 497 GCATGC [GA]CATG^[CT]
PshAI 943 GACTGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1095 GCTCTTC GCTCTTC(1/4)
SphI 497 GCATGC GCATG^C
StuI 779 AGGCCT AGG^CCT
Tsp45I 941 GTGAC ^GT[GC]AC
Tsp509I 731 AATT ^AATT
XhoII 582 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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Alw44I 442 GTGCAC G^TGCAC
621 GTGCAC
ApaLI 442 GTGCAC G^TGCAC
621 GTGCAC
AvaI 46 CCCGGG C^[CT]CG[GA]G
1149 CCCGGG
BsgI 311 GTGCAG GTGCAG(16/14)
785 GTGCAG
BsoBI 46 CCCGGG C^[CT]CG[GA]G
1149 CCCGGG
BspMI 917 ACCTGC ACCTGC(4/8)
1080 GCAGGT
BstXI 254 CCAAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
818 CCAATGCCCTGG
DdeI 85 CTGAG C^T[ACGT]AG
678 CTCAG
HaeII 456 GGCGCT [GA]GCGC^[CT]
800 AGCGCT
MseI 604 TTAA T^TAA
611 TTAA
PstI 229 CTGCAG CTGCA^G
460 CTGCAG
PvuII 523 CAGCTG CAG^CTG
1057 CAGCTG
SfaNI 164 GCATC GCATC(5/9)
425 GCATC
SfcI 229 CTGCAG C^T[GA][CT]AG
460 CTGCAG
SmaI 46 CCCGGG CCC^GGG
1149 CCCGGG
TfiI 285 GAATC G^A[AT]TC
528 GATTC
Tru9I 604 TTAA T^TAA
611 TTAA
XmaCI 46 CCCGGG C^CCGGG
1149 CCCGGG
XmaI 46 CCCGGG C^CCGGG
1149 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
ApaI AspHI AvaII BanII BbvI
BcnI BglI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DpnII DraII EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI TaqI TseI TspRI
TthHB8I