Restriction map for
pF1KB6605
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AclI AfaI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BfaI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsgI BsiEI BsiHKAI BsiWI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FseI HindIII HpaI
KpnI MaeI MamI MfeI MluI
MluNI MscI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 986 TCCGGA T^CCGGA
AcyI 1094 GGCGCC G[GA]^CG[CT]C
AflII 301 CTTAAG C^TTAAG
AlwNI 758 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 141 GGGCCC GGGCC^C
AviII 209 TGCGCA TGC^GCA
BanI 1094 GGCGCC G^G[CT][GA]CC
BanII 141 GGGCCC G[GA]GC[CT]^C
BbeI 1094 GGCGCC GGCGC^C
BclI 339 TGATCA T^GATCA
BcnI 855 CCGGG CC^[GC]GG
BfrI 301 CTTAAG C^TTAAG
BglII 601 AGATCT A^GATCT
BpmI 1018 CTGGAG CTGGAG(16/14)
BsaHI 1094 GGCGCC G[GA]^CG[CT]C
BsaI 1034 GAGACC GGTCTC(1/5)
BsaWI 986 TCCGGA [AT]^CCGG[AT]
BseAI 986 TCCGGA T^CCGGA
BsmFI 219 GTCCC GGGAC(10/14)
BsmI 1162 GCATTC GAATGC(1/-1)
BspEI 986 TCCGGA T^CCGGA
BsrFI 121 GCCGGC [GA]^CCGG[CT]
BstBI 412 TTCGAA TT^CGAA
BstEII 36 GGTCACC G^GT[ACGT]ACC
BstPI 36 GGTCACC G^GT[ACGT]ACC
Cfr10I 121 GCCGGC [GA]^CCGG[CT]
DraIII 395 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1116 GACCGACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1041 TGGCCG [CT]^GGCC[GA]
EcoNI 481 CCTATTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 36 GGTCACC G^GT[ACGT]ACC
EcoT14I 1038 CCTTGG C^C[AT][AT]GG
FbaI 339 TGATCA T^GATCA
FspI 209 TGCGCA TGC^GCA
HgaI 282 GACGC GACGC(5/10)
Hin1I 1094 GGCGCC G[GA]^CG[CT]C
HincII 265 GTTGAC GT[CT]^[GA]AC
HindII 265 GTTGAC GT[CT]^[GA]AC
KasI 1094 GGCGCC G^GCGCC
KspI 170 CCGCGG CCGC^GG
MroI 986 TCCGGA T^CCGGA
MslI 616 CATTACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 121 GCCGGC GCC^GGC
NarI 1094 GGCGCC GG^CGCC
NciI 855 CCGGG CC^[GC]GG
NgoMIV 121 GCCGGC G^CCGGC
NlaIII 955 CATG CATG^
NspV 412 TTCGAA TT^CGAA
PleI 217 GAGTC GAGTC(4/5)
SacII 170 CCGCGG CCGC^GG
SfoI 1094 GGCGCC GGC^GCC
SfuI 412 TTCGAA TT^CGAA
SmlI 301 CTTAAG C^T[CT][GA]AG
StyI 1038 CCTTGG C^C[AT][AT]GG
XcmI 251 CCAGCGTTGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 171 CGCG CG^CG
192 CGCG
AlwI 658 GGATC GGATC(4/5)
702 GGATC
BbsI 332 GAAGAC GAAGAC(2/6)
377 GAAGAC
BmyI 7 GGGCAC G[AGT]GC[ACT]^C
141 GGGCCC
BpuAI 332 GAAGAC GAAGAC(2/6)
377 GAAGAC
BsmBI 1010 CGTCTC CGTCTC(1/5)
280 GAGACG
Bsp1286I 7 GGGCAC G[AGT]GC[ACT]^C
141 GGGCCC
BstUI 171 CGCG CG^CG
192 CGCG
BstYI 601 AGATCT [GA]^GATC[CT]
658 GGATCT
DraII 140 GGGGCCC [GA]G^G[ACGT]CC[CT]
141 GGGCCCT
EcoO109I 140 GGGGCCC [GA]G^G[ACGT]CC[CT]
141 GGGCCCT
FauI 240 CCCGC CCCGC(4/6)
125 GCGGG
HaeII 297 GGCGCT [GA]GCGC^[CT]
1094 GGCGCC
MaeIII 37 GTCAC ^GT[ACGT]AC
715 GTGAC
MflI 601 AGATCT [GA]^GATC[CT]
658 GGATCT
MvnI 171 CGCG CG^CG
192 CGCG
PflMI 1048 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1099 CCAAACCGTGG
PvuII 205 CAGCTG CAG^CTG
1015 CAGCTG
Tsp45I 37 GTCAC ^GT[GC]AC
715 GTGAC
Van91I 1048 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1099 CCAAACCGTGG
XhoII 601 AGATCT [GA]^GATC[CT]
658 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BbvI BsaJI BseRI BsiYI BslI
BsmAI BsrI BssKI BssSI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EarI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIV Sau3AI Sau96I ScrFI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I