Restriction map for
pF1KB6610
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AlwI
AocI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BcgI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccII 121 CGCG CG^CG
AcsI 232 AAATTT [GA]^AATT[CT]
AcyI 951 GGCGCC G[GA]^CG[CT]C
Alw44I 763 GTGCAC G^TGCAC
ApaI 329 GGGCCC GGGCC^C
ApaLI 763 GTGCAC G^TGCAC
ApoI 232 AAATTT [GA]^AATT[CT]
BbeI 951 GGCGCC GGCGC^C
BbsI 489 GAAGAC GAAGAC(2/6)
BpuAI 489 GAAGAC GAAGAC(2/6)
BsaHI 951 GGCGCC G[GA]^CG[CT]C
BsiEI 74 CGACCG CG[GA][CT]^CG
BsrDI 110 GCAATG GCAATG(2/0)
BstEII 57 GGTCACC G^GT[ACGT]ACC
BstPI 57 GGTCACC G^GT[ACGT]ACC
BstUI 121 CGCG CG^CG
BstXI 1268 CCAACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 308 CCATGG C^C[GA][CT]GG
EarI 985 GAAGAG CTCTTC(1/4)
EcoO65I 57 GGTCACC G^GT[ACGT]ACC
Hin1I 951 GGCGCC G[GA]^CG[CT]C
HinfI 754 GAGTC G^A[ACGT]TC
KasI 951 GGCGCC G^GCGCC
Ksp632I 985 GAAGAG CTCTTC(1/4)
MseI 512 TTAA T^TAA
MvnI 121 CGCG CG^CG
NarI 951 GGCGCC GG^CGCC
NcoI 308 CCATGG C^CATGG
NspI 1256 GCATGT [GA]CATG^[CT]
PflMI 447 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 754 GAGTC GAGTC(4/5)
SfoI 951 GGCGCC GGC^GCC
SmlI 915 CTCAAG C^T[CT][GA]AG
StuI 41 AGGCCT AGG^CCT
Tru9I 512 TTAA T^TAA
Tsp509I 233 AATT ^AATT
Van91I 447 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1098 CCAGGGGCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AlwNI 531 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
902 CAGCTGCTG
Aor51HI 30 AGCGCT AGC^GCT
665 AGCGCT
AvaI 768 CCCGAG C^[CT]CG[GA]G
1180 CCCGAG
AvaII 210 GGACC G^G[AT]CC
457 GGACC
BcnI 627 CCGGG CC^[GC]GG
657 CCGGG
BglI 592 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1247 GCCATTTTGGC
BglII 930 AGATCT A^GATCT
1166 AGATCT
BsaAI 46 TACGTG [CT]AC^GT[GA]
1273 TACGTG
BsaI 880 GAGACC GGTCTC(1/5)
1021 GAGACC
BsmAI 880 GAGAC GTCTC(1/5)
1021 GAGAC
BsmFI 456 GGGAC GGGAC(10/14)
69 GTCCC
BsoBI 768 CCCGAG C^[CT]CG[GA]G
1180 CCCGAG
BsrFI 948 GCCGGC [GA]^CCGG[CT]
1063 GCCGGC
BstYI 930 AGATCT [GA]^GATC[CT]
1166 AGATCT
Cfr10I 948 GCCGGC [GA]^CCGG[CT]
1063 GCCGGC
Eco47III 30 AGCGCT AGC^GCT
665 AGCGCT
EcoT14I 308 CCATGG C^C[AT][AT]GG
398 CCAAGG
MaeIII 58 GTCAC ^GT[ACGT]AC
337 GTGAC
MboII 489 GAAGA GAAGA(8/7)
985 GAAGA
MflI 930 AGATCT [GA]^GATC[CT]
1166 AGATCT
MslI 117 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1200 CATCGAGGTG
NaeI 948 GCCGGC GCC^GGC
1063 GCCGGC
NciI 627 CCGGG CC^[GC]GG
657 CCGGG
NgoMIV 948 GCCGGC G^CCGGC
1063 GCCGGC
PpuMI 209 AGGACCC [GA]G^G[AT]CC[CT]
456 GGGACCC
PstI 198 CTGCAG CTGCA^G
856 CTGCAG
PvuII 610 CAGCTG CAG^CTG
902 CAGCTG
SacI 166 GAGCTC GAGCT^C
1279 GAGCTC
SfaNI 380 GCATC GCATC(5/9)
1199 GCATC
StyI 308 CCATGG C^C[AT][AT]GG
398 CCAAGG
TaqI 1202 TCGA T^CGA
1211 TCGA
Tsp45I 58 GTCAC ^GT[GC]AC
337 GTGAC
TthHB8I 1202 TCGA T^CGA
1211 TCGA
XhoII 930 AGATCT [GA]^GATC[CT]
1166 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AspHI BalI
BanI BanII BbvCI BbvI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeII MboI MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfcI TseI
TspRI