Restriction map for
pF1KB6640
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BpmI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI BspDI BspEI
BspHI BsrBI BsrDI BsrFI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI MamI
MflI MluI MroI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspI StuI StyI
SwaI Tth111I Van91I XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 282 ACATGT A^C[GA][CT]GT
AlwI 69 GGATC GGATC(4/5)
AvaII 1254 GGTCC G^G[AT]CC
BalI 270 TGGCCA TGG^CCA
BanI 853 GGCACC G^G[CT][GA]CC
BanII 308 GGGCTC G[GA]GC[CT]^C
BcnI 99 CCGGG CC^[GC]GG
BlpI 310 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 310 GCTCAGC GC^T[ACGT]AGC
BsaAI 1268 TACGTG [CT]AC^GT[GA]
BsaI 28 GGTCTC GGTCTC(1/5)
BseRI 1316 CTCCTC GAGGAG(10/8)
BsmI 682 GAATGC GAATGC(1/-1)
Bsp1407I 1014 TGTACA T^GTACA
BspLU11I 282 ACATGT A^CATGT
BspMI 1078 ACCTGC ACCTGC(4/8)
BsrGI 1014 TGTACA T^GTACA
BssHII 21 GCGCGC G^CGCGC
CelII 310 GCTCAGC GC^T[ACGT]AGC
DraII 1253 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 11 CCGCGG C^C[GA][CT]GG
EaeI 270 TGGCCA [CT]^GGCC[GA]
EcoNI 861 CCTAACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1253 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 899 GAATTC G^AATTC
FauI 13 GCGGG CCCGC(4/6)
HincII 1234 GTTGAC GT[CT]^[GA]AC
HindII 1234 GTTGAC GT[CT]^[GA]AC
KspI 11 CCGCGG CCGC^GG
MaeII 1269 ACGT A^CGT
MluNI 270 TGGCCA TGG^CCA
MscI 270 TGGCCA TGG^CCA
MslI 656 CATGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 11 CCGCGG C[AC]G^C[GT]G
NciI 99 CCGGG CC^[GC]GG
NheI 543 GCTAGC G^CTAGC
NlaIV 853 GGCACC GG[ACGT]^[ACGT]CC
NspI 282 ACATGT [GA]CATG^[CT]
PpuMI 1253 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 257 CTGCAG CTGCA^G
SacII 11 CCGCGG CCGC^GG
SfcI 257 CTGCAG C^T[GA][CT]AG
SpeI 1002 ACTAGT A^CTAGT
SspBI 1014 TGTACA T^GTACA
XbaI 1287 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 12 CGCG CG^CG
22 CGCG
AciI 11 CCGC CCGC(-3/-1)
13 GCGG
Asp700I 780 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1054 GAAATGGTTC
BbsI 427 GAAGAC GAAGAC(2/6)
522 GTCTTC
BbvI 1306 GCAGC GCAGC(8/12)
694 GCTGC
BmyI 308 GGGCTC G[AGT]GC[ACT]^C
1271 GTGCCC
BpuAI 427 GAAGAC GAAGAC(2/6)
522 GTCTTC
BsmAI 29 GTCTC GTCTC(1/5)
1314 GTCTC
Bsp1286I 308 GGGCTC G[AGT]GC[ACT]^C
1271 GTGCCC
BstAPI 259 GCAGTTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1149 GCATTATTTGC
BstUI 12 CGCG CG^CG
22 CGCG
CfoI 21 GCGC GCG^C
23 GCGC
Cfr13I 101 GGGCC G^G[ACGT]CC
1254 GGTCC
EarI 588 CTCTTC CTCTTC(1/4)
808 GAAGAG
Fnu4HI 694 GCTGC GC^[ACGT]GC
1306 GCAGC
HaeIII 102 GGCC GG^CC
271 GGCC
HapII 33 CCGG C^CGG
99 CCGG
HhaI 21 GCGC GCG^C
23 GCGC
HinP1I 21 GCGC G^CGC
23 GCGC
HpaII 33 CCGG C^CGG
99 CCGG
ItaI 694 GCTGC GC^[ACGT]GC
1306 GCAGC
Ksp632I 588 CTCTTC CTCTTC(1/4)
808 GAAGAG
MfeI 457 CAATTG C^AATTG
1190 CAATTG
MspI 33 CCGG C^CGG
99 CCGG
MunI 457 CAATTG C^AATTG
1190 CAATTG
MvnI 12 CGCG CG^CG
22 CGCG
Sau96I 101 GGGCC G^G[ACGT]CC
1254 GGTCC
SfaNI 514 GATGC GCATC(5/9)
117 GCATC
TaqI 152 TCGA T^CGA
897 TCGA
TseI 694 GCTGC G^C[AT]GC
1306 GCAGC
TthHB8I 152 TCGA T^CGA
897 TCGA
XmnI 780 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1054 GAAATGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AcsI AfaI AluI ApoI BfaI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI Cac8I DdeI DpnII
EcoRII FokI HinfI HphI MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII PleI
RsaI Sau3AI ScrFI TfiI Tru9I
Tsp45I Tsp509I TspRI