Restriction map for
pF1KB6679
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoT22I
FbaI FseI FspI HaeII Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MspA1I
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AfaI 1091 GTAC GT^AC
AflIII 1118 ACGTGT A^C[GA][CT]GT
AlwNI 181 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 94 CCCGAG C^[CT]CG[GA]G
BanI 356 GGCACC G^G[CT][GA]CC
BbsI 30 GAAGAC GAAGAC(2/6)
BcnI 902 CCCGG CC^[GC]GG
BpuAI 30 GAAGAC GAAGAC(2/6)
BsiEI 1112 CGATCG CG[GA][CT]^CG
BsmAI 656 GAGAC GTCTC(1/5)
BsmI 716 GAATGC GAATGC(1/-1)
BsoBI 94 CCCGAG C^[CT]CG[GA]G
BspMI 812 ACCTGC ACCTGC(4/8)
BsrDI 1228 GCAATG GCAATG(2/0)
BstEII 1234 GGTTACC G^GT[ACGT]ACC
BstPI 1234 GGTTACC G^GT[ACGT]ACC
BstXI 371 CCAGCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 816 GCGC GCG^C
DraI 556 TTTAAA TTT^AAA
EarI 924 GAAGAG CTCTTC(1/4)
EcoO65I 1234 GGTTACC G^GT[ACGT]ACC
EcoRI 1195 GAATTC G^AATTC
EcoRV 403 GATATC GAT^ATC
FauI 981 CCCGC CCCGC(4/6)
HgaI 735 GACGC GACGC(5/10)
HhaI 816 GCGC GCG^C
HinP1I 816 GCGC G^CGC
HincII 871 GTCAAC GT[CT]^[GA]AC
HindII 871 GTCAAC GT[CT]^[GA]AC
Ksp632I 924 GAAGAG CTCTTC(1/4)
MslI 1050 CATTGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 902 CCCGG CC^[GC]GG
NcoI 1205 CCATGG C^CATGG
NspI 738 GCATGT [GA]CATG^[CT]
PvuI 1112 CGATCG CGAT^CG
RsaI 1091 GTAC GT^AC
SapI 924 GAAGAGC GCTCTTC(1/4)
XcmI 754 CCATGCTGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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BalI 247 TGGCCA TGG^CCA
773 TGGCCA
BglI 855 GCCGCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
965 GCCCCCTGGGC
BsaWI 124 TCCGGT [AT]^CCGG[AT]
302 ACCGGA
BseRI 522 CTCCTC GAGGAG(10/8)
66 GAGGAG
DraII 211 AGGACCC [GA]G^G[ACGT]CC[CT]
809 GGGACCT
EaeI 247 TGGCCA [CT]^GGCC[GA]
773 TGGCCA
EcoNI 326 CCTTTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
813 CCTGCGCCAGG
EcoO109I 211 AGGACCC [GA]G^G[ACGT]CC[CT]
809 GGGACCT
EcoT14I 347 CCTTGG C^C[AT][AT]GG
1205 CCATGG
HphI 366 TCACC GGTGA(8/7)
990 TCACC
MluNI 247 TGGCCA TGG^CCA
773 TGGCCA
MscI 247 TGGCCA TGG^CCA
773 TGGCCA
MwoI 855 GCCGCACAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
965 GCCCCCTGGGC
PleI 145 GACTC GAGTC(4/5)
75 GAGTC
PpuMI 211 AGGACCC [GA]G^G[AT]CC[CT]
809 GGGACCT
StyI 347 CCTTGG C^C[AT][AT]GG
1205 CCATGG
TaqI 1115 TCGA T^CGA
1124 TCGA
TthHB8I 1115 TCGA T^CGA
1124 TCGA
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BanII BbvI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DsaI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspI MvaI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII