Restriction map for
pF1KB6684
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AlwI
AlwNI AocI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BlnI BsaAI BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrFI BsrGI BssHII Bst1107I BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoRV FauI
FseI FspI Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
PacI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Van91I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AciI 955 CCGC CCGC(-3/-1)
AfaI 585 GTAC GT^AC
AflIII 690 ACATGT A^C[GA][CT]GT
Alw44I 790 GTGCAC G^TGCAC
Aor51HI 608 AGCGCT AGC^GCT
ApaI 503 GGGCCC GGGCC^C
ApaLI 790 GTGCAC G^TGCAC
AspI 872 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 421 CTCGAG C^[CT]CG[GA]G
BanI 136 GGCACC G^G[CT][GA]CC
BanII 503 GGGCCC G[GA]GC[CT]^C
BbsI 1206 GTCTTC GAAGAC(2/6)
BclI 774 TGATCA T^GATCA
BglII 425 AGATCT A^GATCT
BlpI 54 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 54 GCTCAGC GC^T[ACGT]AGC
BpuAI 1206 GTCTTC GAAGAC(2/6)
BsaWI 1232 ACCGGA [AT]^CCGG[AT]
BseRI 160 CTCCTC GAGGAG(10/8)
BsgI 1257 CTGCAC GTGCAG(16/14)
BsmI 336 GAATGC GAATGC(1/-1)
BsoBI 421 CTCGAG C^[CT]CG[GA]G
BspLU11I 690 ACATGT A^CATGT
BspMI 751 GCAGGT ACCTGC(4/8)
BsrBI 955 CCGCTC CCGCTC(-3/-3)
BstAPI 331 GCAACGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 174 TTCGAA TT^CGAA
BstEII 1074 GGTTACC G^GT[ACGT]ACC
BstPI 1074 GGTTACC G^GT[ACGT]ACC
BstYI 425 AGATCT [GA]^GATC[CT]
CelII 54 GCTCAGC GC^T[ACGT]AGC
DrdI 697 GACTACTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 608 AGCGCT AGC^GCT
EcoNI 908 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1074 GGTTACC G^GT[ACGT]ACC
EcoRI 816 GAATTC G^AATTC
EcoT22I 1012 ATGCAT ATGCA^T
FbaI 774 TGATCA T^GATCA
HapII 1233 CCGG C^CGG
HgaI 872 GACGC GACGC(5/10)
HincII 706 GTCAAC GT[CT]^[GA]AC
HindII 706 GTCAAC GT[CT]^[GA]AC
HpaII 1233 CCGG C^CGG
MaeII 509 ACGT A^CGT
MflI 425 AGATCT [GA]^GATC[CT]
MspI 1233 CCGG C^CGG
NcoI 264 CCATGG C^CATGG
NsiI 1012 ATGCAT ATGCA^T
NspV 174 TTCGAA TT^CGAA
PaeR7I 421 CTCGAG C^TCGAG
PflFI 872 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 380 GAGTC GAGTC(4/5)
PpuMI 9 AGGTCCC [GA]G^G[AT]CC[CT]
RsaI 585 GTAC GT^AC
SfcI 374 CTACAG C^T[GA][CT]AG
SfuI 174 TTCGAA TT^CGAA
SmlI 421 CTCGAG C^T[CT][GA]AG
SphI 1010 GCATGC GCATG^C
Tth111I 872 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 717 CCAAGACCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 421 CTCGAG C^TCGAG
XhoII 425 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 816 GAATTC [GA]^AATT[CT]
1192 AAATTC
ApoI 816 GAATTC [GA]^AATT[CT]
1192 AAATTC
AspHI 790 GTGCAC G[AT]GC[AT]^C
998 GTGCTC
BfaI 182 CTAG C^TAG
196 CTAG
BpmI 620 CTGGAG CTGGAG(16/14)
811 CTGGAG
BsiHKAI 790 GTGCAC G[AT]GC[AT]^C
998 GTGCTC
BssSI 588 CACGAG CACGAG(-5/-1)
1024 CTCGTG
DsaI 264 CCATGG C^C[GA][CT]GG
353 CCGTGG
HphI 1097 TCACC GGTGA(8/7)
1115 TCACC
MaeI 182 CTAG C^TAG
196 CTAG
MseI 410 TTAA T^TAA
890 TTAA
MspA1I 392 CAGCTG C[AC]G^C[GT]G
544 CAGCTG
NspI 690 ACATGT [GA]CATG^[CT]
1010 GCATGC
PvuII 392 CAGCTG CAG^CTG
544 CAGCTG
SapI 604 GAAGAGC GCTCTTC(1/4)
531 GCTCTTC
StuI 561 AGGCCT AGG^CCT
857 AGGCCT
Tru9I 410 TTAA T^TAA
890 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccI AluI AvaII BbvI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HhaI
HinP1I HinfI ItaI Ksp632I MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I