Restriction map for
pF1KB6687
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspI AvaII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglII
BlnI BpmI BsaAI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
FseI HaeII HapII HindIII HpaI
HpaII HphI KasI KpnI KspI
MfeI MluI MroI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I Van91I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 204 GCGG CCGC(-3/-1)
AclI 613 AACGTT AA^CGTT
AcyI 546 GACGCC G[GA]^CG[CT]C
AhdI 619 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 619 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 580 CCCGAG C^[CT]CG[GA]G
AviII 121 TGCGCA TGC^GCA
BalI 515 TGGCCA TGG^CCA
BbsI 1197 GTCTTC GAAGAC(2/6)
BlpI 168 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 168 GCTAAGC GC^T[ACGT]AGC
BpuAI 1197 GTCTTC GAAGAC(2/6)
BsaBI 334 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 546 GACGCC G[GA]^CG[CT]C
BseRI 1090 GAGGAG GAGGAG(10/8)
BsgI 1139 GTGCAG GTGCAG(16/14)
BsiYI 591 CCTTGGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 591 CCTTGGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 449 GGGAC GGGAC(10/14)
BsoBI 580 CCCGAG C^[CT]CG[GA]G
BstXI 896 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 168 GCTAAGC GC^T[ACGT]AGC
CfoI 122 GCGC GCG^C
DraI 84 TTTAAA TTT^AAA
EaeI 515 TGGCCA [CT]^GGCC[GA]
Eam1105I 619 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 739 GAATTC G^AATTC
EcoRV 74 GATATC GAT^ATC
EcoT14I 591 CCTTGG C^C[AT][AT]GG
EcoT22I 914 ATGCAT ATGCA^T
FauI 204 GCGGG CCCGC(4/6)
FspI 121 TGCGCA TGC^GCA
HgaI 546 GACGC GACGC(5/10)
HhaI 122 GCGC GCG^C
Hin1I 546 GACGCC G[GA]^CG[CT]C
HinP1I 122 GCGC G^CGC
MamI 334 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 515 TGGCCA TGG^CCA
MscI 515 TGGCCA TGG^CCA
MslI 315 CATAGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 914 ATGCAT ATGCA^T
Psp1406I 613 AACGTT AA^CGTT
PstI 524 CTGCAG CTGCA^G
SmlI 107 CTCAAG C^T[CT][GA]AG
StyI 591 CCTTGG C^C[AT][AT]GG
XbaI 1245 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 680 GTAC GT^AC
957 GTAC
AlwNI 400 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1253 CAGCAGCTG
BanII 104 GAGCTC G[GA]GC[CT]^C
325 GAGCTC
BglI 199 GCCAAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
871 GCCAGTCAGGC
BspHI 372 TCATGA T^CATGA
743 TCATGA
BsrI 461 CCAGT ACTGG(1/-1)
872 CCAGT
BssKI 518 CCAGG ^CC[ACGT]GG
676 CCAGG
BstNI 518 CCAGG CC^[AT]GG
676 CCAGG
Cac8I 11 GCAAGC GC[ACGT]^[ACGT]GC
204 GCGGGC
Cfr13I 206 GGGCC G^G[ACGT]CC
1261 GGGCC
EarI 820 CTCTTC CTCTTC(1/4)
1048 GAAGAG
EcoRII 518 CCAGG ^CC[AT]GG
676 CCAGG
HincII 616 GTTGAC GT[CT]^[GA]AC
1206 GTCAAC
HindII 616 GTTGAC GT[CT]^[GA]AC
1206 GTCAAC
Ksp632I 820 CTCTTC CTCTTC(1/4)
1048 GAAGAG
MaeII 614 ACGT A^CGT
776 ACGT
MaeIII 17 GTGAC ^GT[ACGT]AC
559 GTGAC
MspA1I 842 CAGCTG C[AC]G^C[GT]G
1256 CAGCTG
MvaI 518 CCAGG CC^[AT]GG
676 CCAGG
PshAI 505 GACTTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1237 GACAGCAGTC
PvuII 842 CAGCTG CAG^CTG
1256 CAGCTG
RcaI 372 TCATGA T^CATGA
743 TCATGA
RsaI 680 GTAC GT^AC
957 GTAC
SacI 104 GAGCTC GAGCT^C
325 GAGCTC
Sau96I 206 GGGCC G^G[ACGT]CC
1261 GGGCC
ScrFI 518 CCAGG CC^[ACGT]GG
676 CCAGG
Tsp45I 17 GTGAC ^GT[GC]AC
559 GTGAC
TspRI 697 CAGTG CA[GC]TG[ACGT][ACGT]^
1145 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AcsI AluI AlwI ApoI Asp700I
AspHI BbvI BclI BfaI BmyI
BsaJI BsiHKAI Bsp1286I BstF5I BstYI
DdeI DpnII FbaI Fnu4HI FokI
HaeIII HinfI ItaI MaeI MboI
MboII MflI MnlI MseI MwoI
NdeII NlaIII PleI Sau3AI SfaNI
SfcI TfiI Tru9I TseI Tsp509I
XhoII XmnI