Restriction map for
pF1KB0430
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI BalI BamHI BbeI BbrPI
BcgI BclI BfrI BglII BsaAI
BsaBI BsaI BseAI BsiEI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FbaI FseI HindIII
HpaI KasI MamI MluI MluNI
MroI MscI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI TfiI XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 2217 GGTACC G^GTACC
AccI 1064 GTCTAC GT^[AC][GT]AC
AcyI 858 GACGCC G[GA]^CG[CT]C
AflIII 2255 ACATGT A^C[GA][CT]GT
Alw44I 2371 GTGCAC G^TGCAC
ApaLI 2371 GTGCAC G^TGCAC
Asp700I 318 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2217 GGTACC G^GTACC
AviII 465 TGCGCA TGC^GCA
AvrII 1498 CCTAGG C^CTAGG
BfaI 1499 CTAG C^TAG
BlnI 1498 CCTAGG C^CTAGG
BlpI 1603 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1603 GCTAAGC GC^T[ACGT]AGC
BsaHI 858 GACGCC G[GA]^CG[CT]C
BsiWI 2281 CGTACG C^GTACG
BspLU11I 2255 ACATGT A^CATGT
BssHII 8 GCGCGC G^CGCGC
BssSI 2023 CTCGTG CACGAG(-5/-1)
BstAPI 2196 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1044 CCACCTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 356 AGATCC [GA]^GATC[CT]
CelII 1603 GCTAAGC GC^T[ACGT]AGC
DraIII 2033 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1055 GACACAGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1324 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1687 GAATTC G^AATTC
FspI 465 TGCGCA TGC^GCA
Hin1I 858 GACGCC G[GA]^CG[CT]C
HincII 979 GTTGAC GT[CT]^[GA]AC
HindII 979 GTTGAC GT[CT]^[GA]AC
KpnI 2217 GGTACC GGTAC^C
MaeI 1499 CTAG C^TAG
MfeI 735 CAATTG C^AATTG
MflI 356 AGATCC [GA]^GATC[CT]
MseI 2183 TTAA T^TAA
MunI 735 CAATTG C^AATTG
NaeI 4 GCCGGC GCC^GGC
NcoI 1250 CCATGG C^CATGG
NgoMIV 4 GCCGGC G^CCGGC
NspI 2255 ACATGT [GA]CATG^[CT]
PflMI 1861 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 91 GACCCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2167 GAGCTC GAGCT^C
SbfI 444 CCTGCAGG CCTGCA^GG
ScaI 2411 AGTACT AGT^ACT
SfiI 1752 GGCCACATTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2493 CCCGGG CCC^GGG
Sse8387I 444 CCTGCAGG CCTGCA^GG
Tru9I 2183 TTAA T^TAA
Van91I 1861 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 356 AGATCC [GA]^GATC[CT]
XmaCI 2493 CCCGGG C^CCGGG
XmaI 2493 CCCGGG C^CCGGG
XmnI 318 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 321 GAATTT [GA]^AATT[CT]
1687 GAATTC
AlwI 357 GATCC GGATC(4/5)
843 GATCC
AocI 454 CCTGAGG CC^T[ACGT]AGG
811 CCTGAGG
ApaI 217 GGGCCC GGGCC^C
1008 GGGCCC
ApoI 321 GAATTT [GA]^AATT[CT]
1687 GAATTC
AspI 777 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2266 GACTACGTC
AvaI 93 CCCGAG C^[CT]CG[GA]G
2493 CCCGGG
BbsI 1138 GAAGAC GAAGAC(2/6)
2082 GAAGAC
BbvCI 1848 CCTCAGC CCTCAGC(-5/-2)
1908 CCTCAGC
BciVI 1636 GTATCC GTATCC(6/5)
2480 GTATCC
BpuAI 1138 GAAGAC GAAGAC(2/6)
2082 GAAGAC
BsaWI 470 ACCGGA [AT]^CCGG[AT]
1652 TCCGGT
BsgI 1695 CTGCAC GTGCAG(16/14)
2287 CTGCAC
BsoBI 93 CCCGAG C^[CT]CG[GA]G
2493 CCCGGG
BsrDI 645 GCAATG GCAATG(2/0)
830 GCAATG
BsrFI 4 GCCGGC [GA]^CCGG[CT]
1946 ACCGGC
Bsu36I 454 CCTGAGG CC^T[ACGT]AGG
811 CCTGAGG
Cfr10I 4 GCCGGC [GA]^CCGG[CT]
1946 ACCGGC
DpnII 357 GATC ^GATC
843 GATC
Eco81I 454 CCTGAGG CC^T[ACGT]AGG
811 CCTGAGG
HgaI 858 GACGC GACGC(5/10)
1880 GCGTC
MaeII 824 ACGT A^CGT
2270 ACGT
MboI 357 GATC ^GATC
843 GATC
MslI 519 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2097 CATCCTTATG
NdeII 357 GATC ^GATC
843 GATC
PflFI 777 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2266 GACTACGTC
Sau3AI 357 GATC ^GATC
843 GATC
StuI 330 AGGCCT AGG^CCT
1215 AGGCCT
Tth111I 777 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2266 GACTACGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BanI BanII BbvI
BcnI BglI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeIII
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SacII SapI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I