Restriction map for
pF1KB0438
##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AclI AflII Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I AspI
AvrII BbrPI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsiEI BsiWI BsmI Bsp1407I
BspHI BspLU11I BsrGI Bst1107I BstBI
BstZ17I CelII CpoI DraI DraIII
EagI EclXI Eco47III Eco52I EcoRI
EcoRV FbaI FseI HindIII HpaI
MamI MfeI MluI MseI MunI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 61 GACGTC GACGT^C
Acc65I 269 GGTACC G^GTACC
AccI 688 GTCTAC GT^[AC][GT]AC
AcsI 384 GAATTT [GA]^AATT[CT]
AflIII 1308 ACGTGT A^C[GA][CT]GT
AgeI 272 ACCGGT A^CCGGT
AhdI 1692 GACCATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1096 CCTGAGG CC^T[ACGT]AGG
ApoI 384 GAATTT [GA]^AATT[CT]
Asp718I 269 GGTACC G^GTACC
AspEI 1692 GACCATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1182 TGCGCA TGC^GCA
BamHI 2298 GGATCC G^GATCC
BbeI 703 GGCGCC GGCGC^C
BcgI 1282 GCAGAGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BspDI 217 ATCGAT AT^CGAT
BsrDI 994 GCAATG GCAATG(2/0)
BssHII 641 GCGCGC G^CGCGC
BstEII 1207 GGTGACC G^GT[ACGT]ACC
BstPI 1207 GGTGACC G^GT[ACGT]ACC
Bsu36I 1096 CCTGAGG CC^T[ACGT]AGG
ClaI 217 ATCGAT AT^CGAT
DrdI 2259 GACCCAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1692 GACCATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1096 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1207 GGTGACC G^GT[ACGT]ACC
EcoT22I 2402 ATGCAT ATGCA^T
FspI 1182 TGCGCA TGC^GCA
HincII 2391 GTTGAC GT[CT]^[GA]AC
HindII 2391 GTTGAC GT[CT]^[GA]AC
KasI 703 GGCGCC G^GCGCC
KpnI 269 GGTACC GGTAC^C
KspI 261 CCGCGG CCGC^GG
NarI 703 GGCGCC GG^CGCC
NsiI 2402 ATGCAT ATGCA^T
PinAI 272 ACCGGT A^CCGGT
PshAI 426 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 261 CCGCGG CCGC^GG
SapI 1752 GCTCTTC GCTCTTC(1/4)
SfoI 703 GGCGCC GGC^GCC
SphI 2404 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 262 CGCG CG^CG
642 CGCG
AccIII 2397 TCCGGA T^CCGGA
2582 TCCGGA
ApaI 534 GGGCCC GGGCC^C
1242 GGGCCC
AspHI 1750 GTGCTC G[AT]GC[AT]^C
2041 GTGCTC
BbsI 423 GAAGAC GAAGAC(2/6)
1780 GTCTTC
BglI 1244 GCCCGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1583 GCCTGGCAGGC
BpuAI 423 GAAGAC GAAGAC(2/6)
1780 GTCTTC
BsaI 1132 GAGACC GGTCTC(1/5)
2084 GAGACC
BseAI 2397 TCCGGA T^CCGGA
2582 TCCGGA
BsiHKAI 1750 GTGCTC G[AT]GC[AT]^C
2041 GTGCTC
BsmBI 95 GAGACG CGTCTC(1/5)
344 GAGACG
BspEI 2397 TCCGGA T^CCGGA
2582 TCCGGA
BssSI 583 CACGAG CACGAG(-5/-1)
1138 CTCGTG
BstUI 262 CGCG CG^CG
642 CGCG
BstXI 759 CCAATTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1694 CCATAGGTCTGG
BstYI 1655 AGATCC [GA]^GATC[CT]
2298 GGATCC
EcoNI 694 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
984 CCTTCTGCAGG
MflI 1655 AGATCC [GA]^GATC[CT]
2298 GGATCC
MroI 2397 TCCGGA T^CCGGA
2582 TCCGGA
MslI 549 CACGTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1215 CACCGGAGTG
MvnI 262 CGCG CG^CG
642 CGCG
NaeI 177 GCCGGC GCC^GGC
1900 GCCGGC
NcoI 1443 CCATGG C^CATGG
2054 CCATGG
NgoMIV 177 GCCGGC G^CCGGC
1900 GCCGGC
PflMI 1323 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1983 CCAGATGCTGG
Tsp509I 385 AATT ^AATT
761 AATT
Van91I 1323 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1983 CCAGATGCTGG
XcmI 453 CCAGGACCTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1736 CCACACCAGGCCTGG
XhoII 1655 AGATCC [GA]^GATC[CT]
2298 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BsaWI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StuI StyI TaqI
TseI Tsp45I TspRI TthHB8I XmaCI
XmaI