Restriction map for
pF1KB9908
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BbvCI BciVI BclI
BfrI BlnI BsaAI BsaBI BsiEI
BsiWI BsmBI BsmI BspDI BspHI
BspLU11I BsrDI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MluNI MscI MslI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspI StuI SwaI TfiI
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccIII 1059 TCCGGA T^CCGGA
AcsI 321 GAATTT [GA]^AATT[CT]
AcyI 188 GGCGCC G[GA]^CG[CT]C
AflIII 1085 ACACGT A^C[GA][CT]GT
AocI 355 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1229 AGCGCT AGC^GCT
ApoI 321 GAATTT [GA]^AATT[CT]
BbeI 188 GGCGCC GGCGC^C
BbsI 865 GTCTTC GAAGAC(2/6)
BglII 881 AGATCT A^GATCT
BpuAI 865 GTCTTC GAAGAC(2/6)
BsaHI 188 GGCGCC G[GA]^CG[CT]C
BsaWI 1059 TCCGGA [AT]^CCGG[AT]
BseAI 1059 TCCGGA T^CCGGA
Bsp1407I 923 TGTACA T^GTACA
BspEI 1059 TCCGGA T^CCGGA
BsrGI 923 TGTACA T^GTACA
Bsu36I 355 CCTGAGG CC^T[ACGT]AGG
DrdI 1661 GACTCTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1229 AGCGCT AGC^GCT
Eco81I 355 CCTGAGG CC^T[ACGT]AGG
Hin1I 188 GGCGCC G[GA]^CG[CT]C
KasI 188 GGCGCC G^GCGCC
MaeII 1087 ACGT A^CGT
MroI 1059 TCCGGA T^CCGGA
MseI 1343 TTAA T^TAA
NarI 188 GGCGCC GG^CGCC
NcoI 686 CCATGG C^CATGG
PvuII 370 CAGCTG CAG^CTG
SfoI 188 GGCGCC GGC^GCC
SmaI 44 CCCGGG CCC^GGG
SphI 1376 GCATGC GCATG^C
SspBI 923 TGTACA T^GTACA
Tru9I 1343 TTAA T^TAA
Tsp509I 322 AATT ^AATT
XcmI 1603 CCAGACCTGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 44 CCCGGG C^CCGGG
XmaI 44 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AlwI 708 GATCC GGATC(4/5)
662 GGATC
BanI 188 GGCGCC G^G[CT][GA]CC
1039 GGCACC
BanII 4 GAGCTC G[GA]GC[CT]^C
1713 GAGCTC
BcgI 23 CGAACACGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1138 GCAGGCCAGTCG
BglI 180 GCCCATTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
836 GCCAGGCTGGC
BlpI 138 GCTCAGC GC^T[ACGT]AGC
228 GCTGAGC
Bpu1102I 138 GCTCAGC GC^T[ACGT]AGC
228 GCTGAGC
BsaI 760 GAGACC GGTCTC(1/5)
1549 GAGACC
BspMI 902 ACCTGC ACCTGC(4/8)
1629 ACCTGC
BssSI 216 CACGAG CACGAG(-5/-1)
448 CACGAG
BstYI 707 AGATCC [GA]^GATC[CT]
881 AGATCT
CelII 138 GCTCAGC GC^T[ACGT]AGC
228 GCTGAGC
EaeI 260 TGGCCG [CT]^GGCC[GA]
966 CGGCCA
EcoNI 420 CCTTTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1269 CCTCTGCAAGG
EcoT14I 686 CCATGG C^C[AT][AT]GG
764 CCAAGG
HgaI 769 GACGC GACGC(5/10)
239 GCGTC
HinfI 569 GAGTC G^A[ACGT]TC
1661 GACTC
MflI 707 AGATCC [GA]^GATC[CT]
881 AGATCT
PaeR7I 7 CTCGAG C^TCGAG
145 CTCGAG
PleI 1661 GACTC GAGTC(4/5)
569 GAGTC
SacI 4 GAGCTC GAGCT^C
1713 GAGCTC
SapI 127 GAAGAGC GCTCTTC(1/4)
1710 GAAGAGC
StyI 686 CCATGG C^C[AT][AT]GG
764 CCAAGG
XhoI 7 CTCGAG C^TCGAG
145 CTCGAG
XhoII 707 AGATCC [GA]^GATC[CT]
881 AGATCT
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI AvaII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TseI Tsp45I TspRI
TthHB8I Van91I