Restriction map for
pF1KE0505
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
Alw44I AlwNI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BseAI BsiEI BsiHKAI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI HphI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AfaI 186 GTAC GT^AC
AhdI 594 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 162 AGCT AG^CT
AlwI 552 GGATC GGATC(4/5)
AocI 379 CCTGAGG CC^T[ACGT]AGG
Aor51HI 251 AGCGCT AGC^GCT
ApaI 27 GGGCCC GGGCC^C
AspEI 594 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 27 GGGCCC G[GA]GC[CT]^C
BbvCI 189 CCTCAGC CCTCAGC(-5/-2)
BglII 504 AGATCT A^GATCT
BmyI 27 GGGCCC G[AGT]GC[ACT]^C
BsaJI 24 CCTGGG C^C[ACGT][ACGT]GG
BsaWI 645 ACCGGA [AT]^CCGG[AT]
BsmBI 273 GAGACG CGTCTC(1/5)
Bsp1286I 27 GGGCCC G[AGT]GC[ACT]^C
BspMI 417 GCAGGT ACCTGC(4/8)
BsrBI 243 CCGCTC CCGCTC(-3/-3)
BstAPI 136 GCAAAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 125 CATCC GGATG(2/0)
BstXI 227 CCAAAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 379 CCTGAGG CC^T[ACGT]AGG
CfoI 252 GCGC GCG^C
Eam1105I 594 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 251 AGCGCT AGC^GCT
Eco81I 379 CCTGAGG CC^T[ACGT]AGG
FokI 125 CATCC GGATG(9/13)
HaeII 251 AGCGCT [GA]GCGC^[CT]
HhaI 252 GCGC GCG^C
HinP1I 252 GCGC G^CGC
HinfI 93 GATTC G^A[ACGT]TC
MaeII 454 ACGT A^CGT
MaeIII 50 GTGAC ^GT[ACGT]AC
MspA1I 33 CAGCGG C[AC]G^C[GT]G
PshAI 394 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 57 CTGCAG CTGCA^G
RsaI 186 GTAC GT^AC
SfcI 57 CTGCAG C^T[GA][CT]AG
TaqI 500 TCGA T^CGA
TfiI 93 GATTC G^A[AT]TC
Tsp45I 50 GTGAC ^GT[GC]AC
Tsp509I 149 AATT ^AATT
TthHB8I 500 TCGA T^CGA
XbaI 294 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AvaII 73 GGACC G^G[AT]CC
307 GGTCC
BfaI 295 CTAG C^TAG
736 CTAG
BsaI 601 GGTCTC GGTCTC(1/5)
701 GGTCTC
BseRI 349 GAGGAG GAGGAG(10/8)
442 GAGGAG
BssKI 24 CCTGG ^CC[ACGT]GG
729 CCAGG
BstNI 24 CCTGG CC^[AT]GG
729 CCAGG
BstYI 504 AGATCT [GA]^GATC[CT]
552 GGATCT
DpnII 505 GATC ^GATC
553 GATC
EcoRII 24 CCTGG ^CC[AT]GG
729 CCAGG
FauI 242 CCCGC CCCGC(4/6)
35 GCGGG
HapII 646 CCGG C^CGG
747 CCGG
HpaII 646 CCGG C^CGG
747 CCGG
MaeI 295 CTAG C^TAG
736 CTAG
MboI 505 GATC ^GATC
553 GATC
MflI 504 AGATCT [GA]^GATC[CT]
552 GGATCT
MseI 259 TTAA T^TAA
689 TTAA
MspI 646 CCGG C^CGG
747 CCGG
MvaI 24 CCTGG CC^[AT]GG
729 CCAGG
NdeII 505 GATC ^GATC
553 GATC
PflMI 248 CCAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
489 CCACCATTTGG
PpuMI 72 GGGACCC [GA]G^G[AT]CC[CT]
306 AGGTCCC
Sau3AI 505 GATC ^GATC
553 GATC
ScrFI 24 CCTGG CC^[ACGT]GG
729 CCAGG
SfaNI 213 GATGC GCATC(5/9)
124 GCATC
Tru9I 259 TTAA T^TAA
689 TTAA
Van91I 248 CCAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
489 CCACCATTTGG
XhoII 504 AGATCT [GA]^GATC[CT]
552 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AcyI BbsI BbvI BpuAI
BsaHI BsgI BsiYI BslI BsmAI
BsmFI BspHI Cac8I Cfr13I DdeI
DraII EcoO109I Fnu4HI HaeIII HgaI
Hin1I ItaI MboII MnlI MslI
MwoI NlaIII NlaIV RcaI Sau96I
TseI TspRI