##################################
# NO CUT ENZYMES (  197 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BamHI       BanII       
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiWI       BsiYI       BslI        BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstXI       
BstYI       BstZ17I     CelII       CfoI        Cfr10I      
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       FauI        FbaI        Fnu4HI      
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaI        HpaII       HphI        ItaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MamI        MfeI        MflI        MluI        
MroI        MspA1I      MspI        MunI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        ScrFI       SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        TseI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AcsI          532  AAATTT               [GA]^AATT[CT]
AlwI          397  GGATC                GGATC(4/5)
AocI          513  CCTGAGG              CC^T[ACGT]AGG
ApoI          532  AAATTT               [GA]^AATT[CT]
AspHI          60  GTGCTC               G[AT]GC[AT]^C
BalI           11  TGGCCA               TGG^CCA
BanI          103  GGTGCC               G^G[CT][GA]CC
BsgI          204  CTGCAC               GTGCAG(16/14)
BsiHKAI        60  GTGCTC               G[AT]GC[AT]^C
BspDI         160  ATCGAT               AT^CGAT
BspMI         338  GCAGGT               ACCTGC(4/8)
BsrDI          39  CATTGC               GCAATG(2/0)
BsrI          118  ACTGG                ACTGG(1/-1)
Bsu36I        513  CCTGAGG              CC^T[ACGT]AGG
Cac8I         125  GCATGC               GC[ACGT]^[ACGT]GC
ClaI          160  ATCGAT               AT^CGAT
EaeI           11  TGGCCA               [CT]^GGCC[GA]
Eco81I        513  CCTGAGG              CC^T[ACGT]AGG
EcoT22I       123  ATGCAT               ATGCA^T
HindIII       347  AAGCTT               A^AGCTT
MluNI          11  TGGCCA               TGG^CCA
MscI           11  TGGCCA               TGG^CCA
NsiI          123  ATGCAT               ATGCA^T
NspI          125  GCATGC               [GA]CATG^[CT]
SphI          125  GCATGC               GCATG^C
TaqI          161  TCGA                 T^CGA
Tsp45I        176  GTGAC                ^GT[GC]AC
TthHB8I       161  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AvaII         341  GGTCC                G^G[AT]CC
              437  GGTCC               
BmyI           60  GTGCTC               G[AGT]GC[ACT]^C
              265  GTGCCC              
BsmAI         132  GAGAC                GTCTC(1/5)
               48  GTCTC               
Bsp1286I       60  GTGCTC               G[AGT]GC[ACT]^C
              265  GTGCCC              
DsaI          107  CCATGG               C^C[GA][CT]GG
              440  CCATGG              
MboII         427  TCTTC                GAAGA(8/7)
              460  TCTTC               
MseI          502  TTAA                 T^TAA
              530  TTAA                
MslI           39  CATTGCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              258  CATCATTGTG          
NcoI          107  CCATGG               C^CATGG
              440  CCATGG              
SfcI           53  CTACAG               C^T[GA][CT]AG
              507  CTACAG              
Tru9I         502  TTAA                 T^TAA
              530  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AluI        BpmI        BsaJI       BstF5I      Cfr13I      
DdeI        DpnII       EcoT14I     FokI        HaeIII      
MaeIII      MboI        MnlI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      SfaNI       StyI        
Tsp509I     TspRI