Restriction map for
pF1KE0879
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AvaI AvaII AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeI MfeI MflI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AcsI 583 GAATTT [GA]^AATT[CT]
AlwI 591 GATCC GGATC(4/5)
AocI 486 CCTGAGG CC^T[ACGT]AGG
ApoI 583 GAATTT [GA]^AATT[CT]
Asp700I 221 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 460 GTGCTC G[AT]GC[AT]^C
AspI 10 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 105 CCTCAGC CCTCAGC(-5/-2)
BmyI 460 GTGCTC G[AGT]GC[ACT]^C
BpmI 688 CTCCAG CTGGAG(16/14)
BsaBI 591 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 640 CTCCTC GAGGAG(10/8)
BsiHKAI 460 GTGCTC G[AT]GC[AT]^C
BsmFI 448 GGGAC GGGAC(10/14)
Bsp1286I 460 GTGCTC G[AGT]GC[ACT]^C
BspLU11I 715 ACATGT A^CATGT
Bsu36I 486 CCTGAGG CC^T[ACGT]AGG
Cac8I 695 GCAGGC GC[ACGT]^[ACGT]GC
Cfr13I 412 GGCCC G^G[ACGT]CC
Eco81I 486 CCTGAGG CC^T[ACGT]AGG
MaeII 76 ACGT A^CGT
MamI 591 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MwoI 346 GCTGCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 715 ACATGT [GA]CATG^[CT]
PflFI 10 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 237 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I 412 GGCCC G^G[ACGT]CC
SmlI 700 CTCAAG C^T[CT][GA]AG
StuI 704 AGGCCT AGG^CCT
Tth111I 10 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 237 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 221 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 74 ACACGT A^C[GA][CT]GT
715 ACATGT
BalI 2 TGGCCA TGG^CCA
320 TGGCCA
BbvI 346 GCTGC GCAGC(8/12)
618 GCTGC
BsmAI 71 GAGAC GTCTC(1/5)
307 GTCTC
BstF5I 265 CATCC GGATG(2/0)
477 CATCC
DpnII 135 GATC ^GATC
591 GATC
EaeI 2 TGGCCA [CT]^GGCC[GA]
320 TGGCCA
Fnu4HI 346 GCTGC GC^[ACGT]GC
618 GCTGC
FokI 265 CATCC GGATG(9/13)
477 CATCC
HinfI 537 GATTC G^A[ACGT]TC
678 GATTC
ItaI 346 GCTGC GC^[ACGT]GC
618 GCTGC
MboI 135 GATC ^GATC
591 GATC
MluNI 2 TGGCCA TGG^CCA
320 TGGCCA
MscI 2 TGGCCA TGG^CCA
320 TGGCCA
MseI 551 TTAA T^TAA
887 TTAA
NcoI 5 CCATGG C^CATGG
350 CCATGG
NdeII 135 GATC ^GATC
591 GATC
Sau3AI 135 GATC ^GATC
591 GATC
TfiI 537 GATTC G^A[AT]TC
678 GATTC
Tru9I 551 TTAA T^TAA
887 TTAA
TseI 346 GCTGC G^C[AT]GC
618 GCTGC
Tsp45I 16 GTCAC ^GT[GC]AC
739 GTGAC
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI BsaJI BsiYI BslI BsrI
BssKI BstNI DdeI DsaI EcoRII
EcoT14I HaeIII HphI MaeIII MboII
MnlI MslI MvaI NlaIII ScrFI
StyI Tsp509I TspRI