Restriction map for
pF1KE0889
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV FauI FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MluI MroI MspA1I MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Asp700I 855 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 464 GTGCTC G[AT]GC[AT]^C
AvaII 440 GGTCC G^G[AT]CC
BalI 371 TGGCCA TGG^CCA
BamHI 724 GGATCC G^GATCC
BanI 544 GGTGCC G^G[CT][GA]CC
BpmI 676 CTCCAG CTGGAG(16/14)
BsaI 769 GGTCTC GGTCTC(1/5)
BsiHKAI 464 GTGCTC G[AT]GC[AT]^C
BsmAI 770 GTCTC GTCTC(1/5)
BsmFI 232 GTCCC GGGAC(10/14)
BspHI 404 TCATGA T^CATGA
BspMI 125 ACCTGC ACCTGC(4/8)
BsrI 417 CCAGT ACTGG(1/-1)
BstEII 666 GGTCACC G^GT[ACGT]ACC
BstPI 666 GGTCACC G^GT[ACGT]ACC
BstXI 110 CCACGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 724 GGATCC [GA]^GATC[CT]
Cac8I 69 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 926 GCGC GCG^C
DpnII 725 GATC ^GATC
DraII 439 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 110 CCACGG C^C[GA][CT]GG
EcoO109I 439 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 666 GGTCACC G^GT[ACGT]ACC
EcoT22I 288 ATGCAT ATGCA^T
HapII 57 CCGG C^CGG
HhaI 926 GCGC GCG^C
HinP1I 926 GCGC G^CGC
HpaII 57 CCGG C^CGG
MboI 725 GATC ^GATC
MflI 724 GGATCC [GA]^GATC[CT]
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MseI 890 TTAA T^TAA
MspI 57 CCGG C^CGG
NdeII 725 GATC ^GATC
NsiI 288 ATGCAT ATGCA^T
PpuMI 439 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 404 TCATGA T^CATGA
Sau3AI 725 GATC ^GATC
TfiI 681 GATTC G^A[AT]TC
Tru9I 890 TTAA T^TAA
XhoII 724 GGATCC [GA]^GATC[CT]
XmnI 855 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 102 GTAC GT^AC
177 GTAC
AluI 149 AGCT AG^CT
494 AGCT
AlwI 725 GATCC GGATC(4/5)
724 GGATC
BanII 3 GAGCCC G[GA]GC[CT]^C
896 GAGCCC
BfaI 151 CTAG C^TAG
268 CTAG
BseRI 37 CTCCTC GAGGAG(10/8)
481 CTCCTC
EaeI 58 CGGCCA [CT]^GGCC[GA]
371 TGGCCA
EcoT14I 540 CCTTGG C^C[AT][AT]GG
692 CCAAGG
MaeI 151 CTAG C^TAG
268 CTAG
NdeI 356 CATATG CA^TATG
699 CATATG
NspI 98 GCATGT [GA]CATG^[CT]
251 ACATGC
RsaI 102 GTAC GT^AC
177 GTAC
SmlI 553 CTCAAG C^T[CT][GA]AG
912 CTTGAG
StyI 540 CCTTGG C^C[AT][AT]GG
692 CCAAGG
Tsp509I 762 AATT ^AATT
816 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MboII
MnlI MslI MvaI MwoI NlaIII
NlaIV PleI Sau96I ScrFI SfaNI
TseI Tsp45I TspRI