Restriction map for pF1KE0889
##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoRI       EcoRV       FauI        FbaI        
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeII       MamI        
MfeI        MluI        MroI        MspA1I      MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

Asp700I       855  GAACCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         464  GTGCTC               G[AT]GC[AT]^C
AvaII         440  GGTCC                G^G[AT]CC
BalI          371  TGGCCA               TGG^CCA
BamHI         724  GGATCC               G^GATCC
BanI          544  GGTGCC               G^G[CT][GA]CC
BpmI          676  CTCCAG               CTGGAG(16/14)
BsaI          769  GGTCTC               GGTCTC(1/5)
BsiHKAI       464  GTGCTC               G[AT]GC[AT]^C
BsmAI         770  GTCTC                GTCTC(1/5)
BsmFI         232  GTCCC                GGGAC(10/14)
BspHI         404  TCATGA               T^CATGA
BspMI         125  ACCTGC               ACCTGC(4/8)
BsrI          417  CCAGT                ACTGG(1/-1)
BstEII        666  GGTCACC              G^GT[ACGT]ACC
BstPI         666  GGTCACC              G^GT[ACGT]ACC
BstXI         110  CCACGGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         724  GGATCC               [GA]^GATC[CT]
Cac8I          69  GCAGGC               GC[ACGT]^[ACGT]GC
CfoI          926  GCGC                 GCG^C
DpnII         725  GATC                 ^GATC
DraII         439  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI          110  CCACGG               C^C[GA][CT]GG
EcoO109I      439  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       666  GGTCACC              G^GT[ACGT]ACC
EcoT22I       288  ATGCAT               ATGCA^T
HapII          57  CCGG                 C^CGG
HhaI          926  GCGC                 GCG^C
HinP1I        926  GCGC                 G^CGC
HpaII          57  CCGG                 C^CGG
MboI          725  GATC                 ^GATC
MflI          724  GGATCC               [GA]^GATC[CT]
MluNI         371  TGGCCA               TGG^CCA
MscI          371  TGGCCA               TGG^CCA
MseI          890  TTAA                 T^TAA
MspI           57  CCGG                 C^CGG
NdeII         725  GATC                 ^GATC
NsiI          288  ATGCAT               ATGCA^T
PpuMI         439  GGGTCCT              [GA]G^G[AT]CC[CT]
RcaI          404  TCATGA               T^CATGA
Sau3AI        725  GATC                 ^GATC
TfiI          681  GATTC                G^A[AT]TC
Tru9I         890  TTAA                 T^TAA
XhoII         724  GGATCC               [GA]^GATC[CT]
XmnI          855  GAACCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AfaI          102  GTAC                 GT^AC
              177  GTAC                
AluI          149  AGCT                 AG^CT
              494  AGCT                
AlwI          725  GATCC                GGATC(4/5)
              724  GGATC               
BanII           3  GAGCCC               G[GA]GC[CT]^C
              896  GAGCCC              
BfaI          151  CTAG                 C^TAG
              268  CTAG                
BseRI          37  CTCCTC               GAGGAG(10/8)
              481  CTCCTC              
EaeI           58  CGGCCA               [CT]^GGCC[GA]
              371  TGGCCA              
EcoT14I       540  CCTTGG               C^C[AT][AT]GG
              692  CCAAGG              
MaeI          151  CTAG                 C^TAG
              268  CTAG                
NdeI          356  CATATG               CA^TATG
              699  CATATG              
NspI           98  GCATGT               [GA]CATG^[CT]
              251  ACATGC              
RsaI          102  GTAC                 GT^AC
              177  GTAC                
SmlI          553  CTCAAG               C^T[CT][GA]AG
              912  CTTGAG              
StyI          540  CCTTGG               C^C[AT][AT]GG
              692  CCAAGG              
Tsp509I       762  AATT                 ^AATT
              816  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

BbvI        BmyI        BsaJI       BsiYI       BslI        
Bsp1286I    BssKI       BstF5I      BstNI       Cfr13I      
DdeI        EcoRII      Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        MaeIII      MboII       
MnlI        MslI        MvaI        MwoI        NlaIII      
NlaIV       PleI        Sau96I      ScrFI       SfaNI       
TseI        Tsp45I      TspRI       
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