################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Asp700I 855 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 464 GTGCTC G[AT]GC[AT]^C AvaII 440 GGTCC G^G[AT]CC BalI 371 TGGCCA TGG^CCA BamHI 724 GGATCC G^GATCC BanI 544 GGTGCC G^G[CT][GA]CC BpmI 676 CTCCAG CTGGAG(16/14) BsaI 769 GGTCTC GGTCTC(1/5) BsiHKAI 464 GTGCTC G[AT]GC[AT]^C BsmAI 770 GTCTC GTCTC(1/5) BsmFI 232 GTCCC GGGAC(10/14) BspHI 404 TCATGA T^CATGA BspMI 125 ACCTGC ACCTGC(4/8) BsrI 417 CCAGT ACTGG(1/-1) BstEII 666 GGTCACC G^GT[ACGT]ACC BstPI 666 GGTCACC G^GT[ACGT]ACC BstXI 110 CCACGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 724 GGATCC [GA]^GATC[CT] Cac8I 69 GCAGGC GC[ACGT]^[ACGT]GC CfoI 926 GCGC GCG^C DpnII 725 GATC ^GATC DraII 439 GGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 110 CCACGG C^C[GA][CT]GG EcoO109I 439 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoO65I 666 GGTCACC G^GT[ACGT]ACC EcoT22I 288 ATGCAT ATGCA^T HapII 57 CCGG C^CGG HhaI 926 GCGC GCG^C HinP1I 926 GCGC G^CGC HpaII 57 CCGG C^CGG MboI 725 GATC ^GATC MflI 724 GGATCC [GA]^GATC[CT] MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA MseI 890 TTAA T^TAA MspI 57 CCGG C^CGG NdeII 725 GATC ^GATC NsiI 288 ATGCAT ATGCA^T PpuMI 439 GGGTCCT [GA]G^G[AT]CC[CT] RcaI 404 TCATGA T^CATGA Sau3AI 725 GATC ^GATC TfiI 681 GATTC G^A[AT]TC Tru9I 890 TTAA T^TAA XhoII 724 GGATCC [GA]^GATC[CT] XmnI 855 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AfaI 102 GTAC GT^AC 177 GTAC AluI 149 AGCT AG^CT 494 AGCT AlwI 725 GATCC GGATC(4/5) 724 GGATC BanII 3 GAGCCC G[GA]GC[CT]^C 896 GAGCCC BfaI 151 CTAG C^TAG 268 CTAG BseRI 37 CTCCTC GAGGAG(10/8) 481 CTCCTC EaeI 58 CGGCCA [CT]^GGCC[GA] 371 TGGCCA EcoT14I 540 CCTTGG C^C[AT][AT]GG 692 CCAAGG MaeI 151 CTAG C^TAG 268 CTAG NdeI 356 CATATG CA^TATG 699 CATATG NspI 98 GCATGT [GA]CATG^[CT] 251 ACATGC RsaI 102 GTAC GT^AC 177 GTAC SmlI 553 CTCAAG C^T[CT][GA]AG 912 CTTGAG StyI 540 CCTTGG C^C[AT][AT]GG 692 CCAAGG Tsp509I 762 AATT ^AATT 816 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboII MnlI MslI MvaI MwoI NlaIII NlaIV PleI Sau96I ScrFI SfaNI TseI Tsp45I TspRI