Restriction map for
pF1KE0970
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# NO CUT ENZYMES ( 116 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AhdI AocI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BbeI
BbvCI BcgI BciVI BclI BfrI
BlnI BpmI BsaBI BsaI BsaWI
BseAI BsiWI BsoBI Bsp1407I BspDI
BspEI BspHI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HpaI HphI KasI KpnI
KspI MamI MfeI MluI MroI
MseI MunI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PstI RcaI RsrII SacII
SalI SapI SbfI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 847 GACGTC GACGT^C
AcsI 1021 GAATTC [GA]^AATT[CT]
AflIII 1367 ACATGT A^C[GA][CT]GT
Aor51HI 1389 AGCGCT AGC^GCT
ApoI 1021 GAATTC [GA]^AATT[CT]
BalI 2 TGGCCA TGG^CCA
BbrPI 1267 CACGTG CAC^GTG
BbsI 1296 GAAGAC GAAGAC(2/6)
BfaI 1393 CTAG C^TAG
BlpI 1396 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1396 GCTGAGC GC^T[ACGT]AGC
BpuAI 1296 GAAGAC GAAGAC(2/6)
BsaAI 1267 CACGTG [CT]AC^GT[GA]
BsgI 352 CTGCAC GTGCAG(16/14)
BsiEI 1150 CGATCG CG[GA][CT]^CG
BsmBI 241 CGTCTC CGTCTC(1/5)
BsmI 693 GAATGC GAATGC(1/-1)
BspLU11I 1367 ACATGT A^CATGT
BsrBI 772 GAGCGG CCGCTC(-3/-3)
BsrDI 935 GCAATG GCAATG(2/0)
CelII 1396 GCTGAGC GC^T[ACGT]AGC
Eco47III 1389 AGCGCT AGC^GCT
EcoRI 1021 GAATTC G^AATTC
EcoT14I 993 CCATGG C^C[AT][AT]GG
HgaI 240 GCGTC GACGC(5/10)
HincII 1165 GTCAAC GT[CT]^[GA]AC
HindII 1165 GTCAAC GT[CT]^[GA]AC
HindIII 19 AAGCTT A^AGCTT
MaeI 1393 CTAG C^TAG
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 279 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 993 CCATGG C^CATGG
NheI 1392 GCTAGC G^CTAGC
PleI 1159 GACTC GAGTC(4/5)
PmaCI 1267 CACGTG CAC^GTG
PmlI 1267 CACGTG CAC^GTG
PshAI 135 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1150 CGATCG CGAT^CG
SfcI 69 CTACAG C^T[GA][CT]AG
SfiI 468 GGCCTCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 16 CTCAAG C^T[CT][GA]AG
StuI 1457 AGGCCT AGG^CCT
StyI 993 CCATGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcyI 239 GGCGTC G[GA]^CG[CT]C
847 GACGTC
Alw44I 220 GTGCAC G^TGCAC
913 GTGCAC
ApaLI 220 GTGCAC G^TGCAC
913 GTGCAC
AspI 1159 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1525 GACCGGGTC
BanI 271 GGCACC G^G[CT][GA]CC
1336 GGCACC
BsaHI 239 GGCGTC G[GA]^CG[CT]C
847 GACGTC
DsaI 767 CCACGG C^C[GA][CT]GG
993 CCATGG
EarI 171 GAAGAG CTCTTC(1/4)
1074 GAAGAG
EcoNI 474 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
573 CCTGATGGAGG
Hin1I 239 GGCGTC G[GA]^CG[CT]C
847 GACGTC
Ksp632I 171 GAAGAG CTCTTC(1/4)
1074 GAAGAG
MspA1I 205 CAGCTG C[AC]G^C[GT]G
604 CAGCTG
PflFI 1159 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1525 GACCGGGTC
PflMI 291 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1095 CCAGCGACTGG
PvuII 205 CAGCTG CAG^CTG
604 CAGCTG
ScaI 80 AGTACT AGT^ACT
197 AGTACT
SphI 377 GCATGC GCATG^C
1136 GCATGC
TfiI 749 GAATC G^A[AT]TC
1215 GATTC
Tsp45I 1192 GTCAC ^GT[GC]AC
1371 GTCAC
Tsp509I 1022 AATT ^AATT
1033 AATT
Tth111I 1159 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1525 GACCGGGTC
Van91I 291 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1095 CCAGCGACTGG
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BanII BbvI
BcnI BglI BglII BmyI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeII
MaeIII MboI MboII MflI MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PpuMI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI TspRI TthHB8I
XhoII