Restriction map for
pF1KB3573
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII AccII AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AviII BbeI BbrPI
BbsI BbvCI BclI BfrI BglII
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I CfoI Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HaeII HhaI HinP1I HpaI KasI
Ksp632I KspI MamI MluI MroI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 420 AAATTT [GA]^AATT[CT]
AcyI 1598 GGCGTC G[GA]^CG[CT]C
AflIII 1343 ACACGT A^C[GA][CT]GT
AocI 1399 CCTGAGG CC^T[ACGT]AGG
ApaI 277 GGGCCC GGGCC^C
ApoI 420 AAATTT [GA]^AATT[CT]
AspHI 781 GAGCAC G[AT]GC[AT]^C
BamHI 1536 GGATCC G^GATCC
BcgI 1473 CGATGGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 844 GGATAC GTATCC(6/5)
BglI 1129 GCCTCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 778 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 778 GCTGAGC GC^T[ACGT]AGC
BsaHI 1598 GGCGTC G[GA]^CG[CT]C
BseRI 1506 CTCCTC GAGGAG(10/8)
BsiHKAI 781 GAGCAC G[AT]GC[AT]^C
Bsp1407I 1044 TGTACA T^GTACA
BsrGI 1044 TGTACA T^GTACA
BstYI 1536 GGATCC [GA]^GATC[CT]
Bsu36I 1399 CCTGAGG CC^T[ACGT]AGG
CelII 778 GCTGAGC GC^T[ACGT]AGC
DraIII 245 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 482 CCATGG C^C[GA][CT]GG
Eco81I 1399 CCTGAGG CC^T[ACGT]AGG
EcoNI 561 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1465 ATGCAT ATGCA^T
FauI 119 CCCGC CCCGC(4/6)
Hin1I 1598 GGCGTC G[GA]^CG[CT]C
HincII 1309 GTCGAC GT[CT]^[GA]AC
HindII 1309 GTCGAC GT[CT]^[GA]AC
HindIII 138 AAGCTT A^AGCTT
MaeII 1345 ACGT A^CGT
MfeI 1290 CAATTG C^AATTG
MflI 1536 GGATCC [GA]^GATC[CT]
MseI 431 TTAA T^TAA
MunI 1290 CAATTG C^AATTG
NcoI 482 CCATGG C^CATGG
NsiI 1465 ATGCAT ATGCA^T
PleI 187 GACTC GAGTC(4/5)
PshAI 904 GACCTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1309 GTCGAC G^TCGAC
ScaI 928 AGTACT AGT^ACT
SspBI 1044 TGTACA T^GTACA
Tru9I 431 TTAA T^TAA
XhoII 1536 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1309 GTCGAC GT^[AC][GT]AC
1327 GTAGAC
AvrII 906 CCTAGG C^CTAGG
1086 CCTAGG
BalI 485 TGGCCA TGG^CCA
1377 TGGCCA
BcnI 474 CCGGG CC^[GC]GG
718 CCCGG
BlnI 906 CCTAGG C^CTAGG
1086 CCTAGG
BpmI 1070 CTCCAG CTGGAG(16/14)
1555 CTGGAG
BsrBI 1503 CCGCTC CCGCTC(-3/-3)
683 GAGCGG
BstAPI 109 GCATCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1164 GCAGAGTGTGC
DpnII 1189 GATC ^GATC
1537 GATC
EaeI 485 TGGCCA [CT]^GGCC[GA]
1377 TGGCCA
HapII 474 CCGG C^CGG
719 CCGG
HpaII 474 CCGG C^CGG
719 CCGG
MboI 1189 GATC ^GATC
1537 GATC
MluNI 485 TGGCCA TGG^CCA
1377 TGGCCA
MscI 485 TGGCCA TGG^CCA
1377 TGGCCA
MslI 934 CACCACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1468 CATCCCGATG
MspA1I 489 CAGCTG C[AC]G^C[GT]G
809 CAGCTG
MspI 474 CCGG C^CGG
719 CCGG
NciI 474 CCGGG CC^[GC]GG
718 CCCGG
NdeII 1189 GATC ^GATC
1537 GATC
PpuMI 909 AGGTCCT [GA]G^G[AT]CC[CT]
1284 AGGACCC
PstI 322 CTGCAG CTGCA^G
610 CTGCAG
PvuII 489 CAGCTG CAG^CTG
809 CAGCTG
Sau3AI 1189 GATC ^GATC
1537 GATC
StuI 306 AGGCCT AGG^CCT
1136 AGGCCT
TaqI 1191 TCGA T^CGA
1310 TCGA
TfiI 455 GAATC G^A[AT]TC
597 GATTC
Tsp509I 421 AATT ^AATT
1291 AATT
TthHB8I 1191 TCGA T^CGA
1310 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
Acc65I AciI AfaI AluI AlwI
AlwNI Asp718I AvaII BanI BanII
BbvI BfaI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DraII EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HgaI
HinfI HphI ItaI KpnI MaeI
MaeIII MboII MnlI MvaI MwoI
NlaIII NlaIV PflMI RsaI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TseI Tsp45I TspRI Van91I