Restriction map for
pF1KB3574
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanI BbeI
BcgI BclI BfrI BglI BglII
BlnI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstEII BstPI BstZ17I Bsu36I Cfr10I
CpoI DraI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HaeII Hin1I HindIII KasI
KpnI Ksp632I KspI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 69 enzymes) #
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AccI 667 GTAGAC GT^[AC][GT]AC
AccII 28 CGCG CG^CG
AfaI 1229 GTAC GT^AC
AseI 432 ATTAAT AT^TAAT
AsnI 432 ATTAAT AT^TAAT
Asp700I 1203 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 364 GTGCTC G[AT]GC[AT]^C
BbrPI 1012 CACGTG CAC^GTG
BbvCI 490 CCTCAGC CCTCAGC(-5/-2)
BciVI 956 GGATAC GTATCC(6/5)
BcnI 724 CCGGG CC^[GC]GG
BfaI 1061 CTAG C^TAG
BlpI 631 GCTGAGC GC^T[ACGT]AGC
BpmI 536 CTGGAG CTGGAG(16/14)
Bpu1102I 631 GCTGAGC GC^T[ACGT]AGC
BsaAI 1012 CACGTG [CT]AC^GT[GA]
BsaBI 615 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 245 CCATGG C^C[ACGT][ACGT]GG
BseRI 86 CTCCTC GAGGAG(10/8)
BsgI 893 CTGCAC GTGCAG(16/14)
BsiHKAI 364 GTGCTC G[AT]GC[AT]^C
BspDI 1282 ATCGAT AT^CGAT
BsrDI 1269 CATTGC GCAATG(2/0)
BssSI 367 CTCGTG CACGAG(-5/-1)
BstNI 9 CCTGG CC^[AT]GG
BstUI 28 CGCG CG^CG
BstYI 419 AGATCC [GA]^GATC[CT]
CelII 631 GCTGAGC GC^T[ACGT]AGC
ClaI 1282 ATCGAT AT^CGAT
DraIII 1000 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 60 GACTTGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 245 CCATGG C^C[GA][CT]GG
EcoNI 525 CCTTTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 9 CCTGG ^CC[AT]GG
EcoT14I 245 CCATGG C^C[AT][AT]GG
HapII 724 CCGG C^CGG
HgaI 1099 GCGTC GACGC(5/10)
HpaI 786 GTTAAC GTT^AAC
HpaII 724 CCGG C^CGG
HphI 654 GGTGA GGTGA(8/7)
MaeI 1061 CTAG C^TAG
MaeII 1013 ACGT A^CGT
MaeIII 548 GTGAC ^GT[ACGT]AC
MamI 615 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 419 AGATCC [GA]^GATC[CT]
MslI 1279 CATATCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1024 CAGCTG C[AC]G^C[GT]G
MspI 724 CCGG C^CGG
MvaI 9 CCTGG CC^[AT]GG
MvnI 28 CGCG CG^CG
NciI 724 CCGGG CC^[GC]GG
NcoI 245 CCATGG C^CATGG
NlaIV 807 GGTTCC GG[ACGT]^[ACGT]CC
NspI 636 GCATGC [GA]CATG^[CT]
PacI 433 TTAATTAA TTAAT^TAA
PflMI 325 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1012 CACGTG CAC^GTG
PmlI 1012 CACGTG CAC^GTG
PshBI 432 ATTAAT AT^TAAT
PvuII 1024 CAGCTG CAG^CTG
RsaI 1229 GTAC GT^AC
SfcI 500 CTACAG C^T[GA][CT]AG
SphI 636 GCATGC GCATG^C
StyI 245 CCATGG C^C[AT][AT]GG
TfiI 81 GATTC G^A[AT]TC
Tsp45I 548 GTGAC ^GT[GC]AC
Van91I 325 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 419 AGATCC [GA]^GATC[CT]
XmnI 1203 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 18 GCGG CCGC(-3/-1)
29 GCGG
AlwNI 530 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
898 CAGATGCTG
BalI 1016 TGGCCA TGG^CCA
1106 TGGCCA
BanII 643 GGGCTC G[GA]GC[CT]^C
760 GGGCTC
BbsI 564 GAAGAC GAAGAC(2/6)
69 GTCTTC
BpuAI 564 GAAGAC GAAGAC(2/6)
69 GTCTTC
BsmAI 94 GTCTC GTCTC(1/5)
866 GTCTC
BsmFI 828 GGGAC GGGAC(10/14)
906 GGGAC
BspMI 778 ACCTGC ACCTGC(4/8)
541 GCAGGT
BssKI 9 CCTGG ^CC[ACGT]GG
724 CCGGG
BstBI 258 TTCGAA TT^CGAA
793 TTCGAA
BstXI 818 CCAATATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1019 CCACCCAGCTGG
Cac8I 35 GCAGGC GC[ACGT]^[ACGT]GC
636 GCATGC
CfoI 27 GCGC GCG^C
913 GCGC
DraII 5 AGGGCCT [GA]G^G[ACGT]CC[CT]
989 AGGGCCT
EaeI 1016 TGGCCA [CT]^GGCC[GA]
1106 TGGCCA
EcoO109I 5 AGGGCCT [GA]G^G[ACGT]CC[CT]
989 AGGGCCT
EcoT22I 395 ATGCAT ATGCA^T
1118 ATGCAT
HhaI 27 GCGC GCG^C
913 GCGC
HinP1I 27 GCGC G^CGC
913 GCGC
HincII 319 GTTGAC GT[CT]^[GA]AC
786 GTTAAC
HindII 319 GTTGAC GT[CT]^[GA]AC
786 GTTAAC
MluNI 1016 TGGCCA TGG^CCA
1106 TGGCCA
MscI 1016 TGGCCA TGG^CCA
1106 TGGCCA
NsiI 395 ATGCAT ATGCA^T
1118 ATGCAT
NspV 258 TTCGAA TT^CGAA
793 TTCGAA
PleI 550 GACTC GAGTC(4/5)
864 GAGTC
ScrFI 9 CCTGG CC^[ACGT]GG
724 CCGGG
SfuI 258 TTCGAA TT^CGAA
793 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BmyI BsiYI BslI Bsp1286I BsrI
BstF5I Cfr13I DdeI DpnII Fnu4HI
FokI HaeIII HinfI ItaI MboI
MboII MnlI MseI MwoI NdeII
NlaIII Sau3AI Sau96I SfaNI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I