Restriction map for
pF1KB5284
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# NO CUT ENZYMES ( 185 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BcgI
BciVI BclI BcnI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MaeII MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp45I TspRI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AfaI 318 GTAC GT^AC
AvaII 120 GGACC G^G[AT]CC
BbvCI 301 GCTGAGG CCTCAGC(-5/-2)
BglI 249 GCCAAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 561 CTCCAG CTGGAG(16/14)
BsaI 260 GAGACC GGTCTC(1/5)
BsmAI 260 GAGAC GTCTC(1/5)
BsrI 432 ACTGG ACTGG(1/-1)
BstYI 278 AGATCC [GA]^GATC[CT]
Cac8I 516 GCACGC GC[ACGT]^[ACGT]GC
DrdI 145 GACATGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 71 TGGCCG [CT]^GGCC[GA]
EcoT14I 250 CCAAGG C^C[AT][AT]GG
FauI 39 CCCGC CCCGC(4/6)
HgaI 134 GCGTC GACGC(5/10)
HinfI 448 GAATC G^A[ACGT]TC
HphI 375 GGTGA GGTGA(8/7)
MflI 278 AGATCC [GA]^GATC[CT]
MslI 411 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 40 CCGCTG C[AC]G^C[GT]G
NlaIV 225 GGTTCC GG[ACGT]^[ACGT]CC
PflMI 438 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 318 GTAC GT^AC
StuI 557 AGGCCT AGG^CCT
StyI 250 CCAAGG C^C[AT][AT]GG
TaqI 158 TCGA T^CGA
TfiI 448 GAATC G^A[AT]TC
Tsp509I 127 AATT ^AATT
TthHB8I 158 TCGA T^CGA
Van91I 438 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 278 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AspHI 34 GAGCTC G[AT]GC[AT]^C
331 GAGCTC
AvaI 177 CTCGGG C^[CT]CG[GA]G
229 CCCGAG
BanII 34 GAGCTC G[GA]GC[CT]^C
331 GAGCTC
BbvI 10 GCTGC GCAGC(8/12)
498 GCTGC
BfaI 292 CTAG C^TAG
346 CTAG
BmyI 34 GAGCTC G[AGT]GC[ACT]^C
331 GAGCTC
BseRI 304 GAGGAG GAGGAG(10/8)
523 GAGGAG
BsiHKAI 34 GAGCTC G[AT]GC[AT]^C
331 GAGCTC
BsoBI 177 CTCGGG C^[CT]CG[GA]G
229 CCCGAG
Bsp1286I 34 GAGCTC G[AGT]GC[ACT]^C
331 GAGCTC
BstF5I 198 CATCC GGATG(2/0)
266 CATCC
DdeI 302 CTGAG C^T[ACGT]AG
422 CTAAG
EarI 274 GAAGAG CTCTTC(1/4)
553 GAAGAG
FokI 198 CATCC GGATG(9/13)
266 CATCC
Ksp632I 274 GAAGAG CTCTTC(1/4)
553 GAAGAG
MaeI 292 CTAG C^TAG
346 CTAG
SacI 34 GAGCTC GAGCT^C
331 GAGCTC
TseI 10 GCTGC G^C[AT]GC
498 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI AlwI BsaJI BsiYI
BslI BssKI BstNI Cfr13I DpnII
EcoRII Fnu4HI HaeIII ItaI MboI
MboII MnlI MvaI MwoI NdeII
NlaIII Sau3AI Sau96I ScrFI