Restriction map for
pF1KB5535
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BbrPI BbvCI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI BstAPI
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRV EcoT22I FauI
FseI FspI HgaI HpaI KpnI
KspI MaeII MamI MluI NaeI
NciI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 815 GTATAC GT^[AC][GT]AC
AccII 148 CGCG CG^CG
AccIII 1316 TCCGGA T^CCGGA
AcsI 471 GAATTC [GA]^AATT[CT]
AcyI 58 GGCGCC G[GA]^CG[CT]C
AflII 466 CTTAAG C^TTAAG
AocI 853 CCTCAGG CC^T[ACGT]AGG
ApoI 471 GAATTC [GA]^AATT[CT]
BamHI 1319 GGATCC G^GATCC
BbeI 58 GGCGCC GGCGC^C
BcgI 72 CGACACACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 213 GGATAC GTATCC(6/5)
BclI 510 TGATCA T^GATCA
BfaI 799 CTAG C^TAG
BfrI 466 CTTAAG C^TTAAG
BsaHI 58 GGCGCC G[GA]^CG[CT]C
BsaI 1181 GAGACC GGTCTC(1/5)
BseAI 1316 TCCGGA T^CCGGA
BseRI 1023 CTCCTC GAGGAG(10/8)
BspEI 1316 TCCGGA T^CCGGA
BsrDI 1064 GCAATG GCAATG(2/0)
BsrFI 1291 ACCGGC [GA]^CCGG[CT]
Bst1107I 815 GTATAC GTA^TAC
BstBI 746 TTCGAA TT^CGAA
BstEII 339 GGTGACC G^GT[ACGT]ACC
BstPI 339 GGTGACC G^GT[ACGT]ACC
BstUI 148 CGCG CG^CG
BstZ17I 815 GTATAC GTA^TAC
Bsu36I 853 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1291 ACCGGC [GA]^CCGG[CT]
DraII 10 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 1106 CCATGG C^C[GA][CT]GG
EarI 325 GAAGAG CTCTTC(1/4)
Eco81I 853 CCTCAGG CC^T[ACGT]AGG
EcoO109I 10 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 339 GGTGACC G^GT[ACGT]ACC
EcoRI 471 GAATTC G^AATTC
FbaI 510 TGATCA T^GATCA
HaeII 58 GGCGCC [GA]GCGC^[CT]
Hin1I 58 GGCGCC G[GA]^CG[CT]C
KasI 58 GGCGCC G^GCGCC
Ksp632I 325 GAAGAG CTCTTC(1/4)
MaeI 799 CTAG C^TAG
MaeIII 340 GTGAC ^GT[ACGT]AC
MfeI 1346 CAATTG C^AATTG
MroI 1316 TCCGGA T^CCGGA
MslI 1356 CATAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1346 CAATTG C^AATTG
MvnI 148 CGCG CG^CG
NarI 58 GGCGCC GG^CGCC
NcoI 1106 CCATGG C^CATGG
NdeI 221 CATATG CA^TATG
NspV 746 TTCGAA TT^CGAA
SfaNI 757 GCATC GCATC(5/9)
SfoI 58 GGCGCC GGC^GCC
SfuI 746 TTCGAA TT^CGAA
Tsp45I 340 GTGAC ^GT[GC]AC
XcmI 753 CCAAGCATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
ApaI 10 GGGCCC GGGCC^C
1102 GGGCCC
AvaII 172 GGACC G^G[AT]CC
1092 GGTCC
BalI 243 TGGCCA TGG^CCA
618 TGGCCA
BbsI 1003 GAAGAC GAAGAC(2/6)
118 GTCTTC
BpmI 132 CTCCAG CTGGAG(16/14)
708 CTGGAG
BpuAI 1003 GAAGAC GAAGAC(2/6)
118 GTCTTC
BsaWI 412 ACCGGA [AT]^CCGG[AT]
1316 TCCGGA
BsgI 87 CTGCAC GTGCAG(16/14)
650 CTGCAC
BsmFI 171 GGGAC GGGAC(10/14)
704 GGGAC
BstF5I 758 CATCC GGATG(2/0)
1256 GGATG
BstYI 1157 GGATCT [GA]^GATC[CT]
1319 GGATCC
Cac8I 24 GCTCGC GC[ACGT]^[ACGT]GC
958 GCTTGC
EaeI 243 TGGCCA [CT]^GGCC[GA]
618 TGGCCA
FokI 758 CATCC GGATG(9/13)
1256 GGATG
HincII 154 GTCAAC GT[CT]^[GA]AC
451 GTCAAC
HindII 154 GTCAAC GT[CT]^[GA]AC
451 GTCAAC
HindIII 956 AAGCTT A^AGCTT
1081 AAGCTT
MflI 1157 GGATCT [GA]^GATC[CT]
1319 GGATCC
MluNI 243 TGGCCA TGG^CCA
618 TGGCCA
MscI 243 TGGCCA TGG^CCA
618 TGGCCA
PleI 582 GACTC GAGTC(4/5)
1243 GACTC
PvuII 768 CAGCTG CAG^CTG
1281 CAGCTG
TaqI 627 TCGA T^CGA
747 TCGA
TfiI 809 GAATC G^A[AT]TC
982 GATTC
TthHB8I 627 TCGA T^CGA
747 TCGA
XhoII 1157 GGATCT [GA]^GATC[CT]
1319 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BanI BanII BbvI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BspMI
BsrI BssKI BstNI BstXI CfoI
Cfr13I DdeI DpnII EcoRII EcoT14I
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SmlI
StyI Tru9I TseI Tsp509I TspRI