Restriction map for
pF1KB5539
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BcgI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I CelII CfoI ClaI CpoI
DraI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KpnI KspI MluI
MroI MvnI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflIII 1520 ACATGT A^C[GA][CT]GT
Asp700I 592 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1118 CCCGGG C^[CT]CG[GA]G
BanI 1129 GGCACC G^G[CT][GA]CC
BbvCI 1620 CCTCAGC CCTCAGC(-5/-2)
BciVI 1468 GGATAC GTATCC(6/5)
BsaBI 966 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1576 GAGACC GGTCTC(1/5)
BsgI 740 CTGCAC GTGCAG(16/14)
BsoBI 1118 CCCGGG C^[CT]CG[GA]G
BspLU11I 1520 ACATGT A^CATGT
BspMI 104 GCAGGT ACCTGC(4/8)
BsrBI 127 GAGCGG CCGCTC(-3/-3)
BsrDI 1424 GCAATG GCAATG(2/0)
BsrFI 206 GCCGGC [GA]^CCGG[CT]
BstEII 1282 GGTGACC G^GT[ACGT]ACC
BstPI 1282 GGTGACC G^GT[ACGT]ACC
Cfr10I 206 GCCGGC [GA]^CCGG[CT]
DrdI 24 GACAAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1282 GGTGACC G^GT[ACGT]ACC
HincII 1165 GTTGAC GT[CT]^[GA]AC
HindII 1165 GTTGAC GT[CT]^[GA]AC
MamI 966 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 224 TTAA T^TAA
MspA1I 229 CAGCTG C[AC]G^C[GT]G
NaeI 206 GCCGGC GCC^GGC
NgoMIV 206 GCCGGC G^CCGGC
PstI 620 CTGCAG CTGCA^G
PvuII 229 CAGCTG CAG^CTG
SacI 1587 GAGCTC GAGCT^C
SapI 124 GAAGAGC GCTCTTC(1/4)
SfcI 620 CTGCAG C^T[GA][CT]AG
SmaI 1118 CCCGGG CCC^GGG
SspI 220 AATATT AAT^ATT
TaqI 1113 TCGA T^CGA
Tru9I 224 TTAA T^TAA
TthHB8I 1113 TCGA T^CGA
XcmI 719 CCAGTTCTAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1118 CCCGGG C^CCGGG
XmaI 1118 CCCGGG C^CCGGG
XmnI 592 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 3 GTCTAC GT^[AC][GT]AC
928 GTCTAC
AfaI 918 GTAC GT^AC
1459 GTAC
AocI 1203 CCTCAGG CC^T[ACGT]AGG
1277 CCTCAGG
BalI 1396 TGGCCA TGG^CCA
1535 TGGCCA
BamHI 65 GGATCC G^GATCC
965 GGATCC
BcnI 1118 CCCGG CC^[GC]GG
1119 CCGGG
BseRI 755 CTCCTC GAGGAG(10/8)
770 GAGGAG
BstAPI 79 GCAACAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1264 GCACGGATTGC
BstXI 700 CCAGCATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
969 CCAGATCGTTGG
Bsu36I 1203 CCTCAGG CC^T[ACGT]AGG
1277 CCTCAGG
Eco81I 1203 CCTCAGG CC^T[ACGT]AGG
1277 CCTCAGG
EcoT14I 60 CCTTGG C^C[AT][AT]GG
1133 CCTTGG
FauI 983 CCCGC CCCGC(4/6)
171 GCGGG
HapII 207 CCGG C^CGG
1119 CCGG
HindIII 1003 AAGCTT A^AGCTT
1301 AAGCTT
HpaII 207 CCGG C^CGG
1119 CCGG
MaeII 932 ACGT A^CGT
1564 ACGT
MfeI 994 CAATTG C^AATTG
1475 CAATTG
MluNI 1396 TGGCCA TGG^CCA
1535 TGGCCA
MscI 1396 TGGCCA TGG^CCA
1535 TGGCCA
MslI 780 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
907 CATGCTGATG
MspI 207 CCGG C^CGG
1119 CCGG
MunI 994 CAATTG C^AATTG
1475 CAATTG
NciI 1118 CCCGG CC^[GC]GG
1119 CCGGG
NspI 1062 GCATGT [GA]CATG^[CT]
1520 ACATGT
PleI 753 GACTC GAGTC(4/5)
1322 GACTC
RsaI 918 GTAC GT^AC
1459 GTAC
StyI 60 CCTTGG C^C[AT][AT]GG
1133 CCTTGG
TfiI 413 GAATC G^A[AT]TC
487 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII BanII BbsI BbvI BfaI
BglII BmyI BpmI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MvaI MwoI
NdeII NlaIII NlaIV PpuMI Sau3AI
Sau96I ScrFI SfaNI TseI Tsp45I
Tsp509I TspRI XhoII