Restriction map for
pF1KB5557
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI DraI Eam1105I
Eco81I EcoO65I EcoRV EcoT14I EcoT22I
FbaI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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ApaI 209 GGGCCC GGGCC^C
AspI 331 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 902 CCCGAG C^[CT]CG[GA]G
BanI 406 GGTGCC G^G[CT][GA]CC
BbsI 118 GAAGAC GAAGAC(2/6)
BglI 266 GCCAGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 118 GAAGAC GAAGAC(2/6)
BsaI 698 GAGACC GGTCTC(1/5)
BseRI 1024 CTCCTC GAGGAG(10/8)
BsiEI 504 CGGCCG CG[GA][CT]^CG
BsmFI 73 GTCCC GGGAC(10/14)
BsmI 1079 GAATGC GAATGC(1/-1)
BsoBI 902 CCCGAG C^[CT]CG[GA]G
BspLU11I 478 ACATGT A^CATGT
BsrDI 570 CATTGC GCAATG(2/0)
BstF5I 195 GGATG GGATG(2/0)
BstYI 707 AGATCC [GA]^GATC[CT]
CpoI 469 CGGACCG CG^G[AT]CCG
DraIII 1094 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 121 GACGATGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 504 CGGCCG C^GGCCG
EclXI 504 CGGCCG C^GGCCG
Eco52I 504 CGGCCG C^GGCCG
EcoNI 214 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 641 GAATTC G^AATTC
FokI 195 GGATG GGATG(9/13)
FseI 505 GGCCGGCC GGCCGG^CC
HgaI 71 GCGTC GACGC(5/10)
MflI 707 AGATCC [GA]^GATC[CT]
MspA1I 986 CAGCTG C[AC]G^C[GT]G
NaeI 506 GCCGGC GCC^GGC
NgoMIV 506 GCCGGC G^CCGGC
NspI 478 ACATGT [GA]CATG^[CT]
PflFI 331 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 986 CAGCTG CAG^CTG
RsrII 469 CGGACCG CG^G[AT]CCG
SacI 883 GAGCTC GAGCT^C
SapI 290 GCTCTTC GCTCTTC(1/4)
SexAI 247 ACCAGGT A^CC[AT]GGT
Tsp45I 475 GTCAC ^GT[GC]AC
TspRI 1096 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 331 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 707 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 24 CGCG CG^CG
70 CGCG
AcsI 641 GAATTC [GA]^AATT[CT]
754 AAATTT
AfaI 201 GTAC GT^AC
714 GTAC
AflIII 452 ACACGT A^C[GA][CT]GT
478 ACATGT
Aor51HI 1197 AGCGCT AGC^GCT
1280 AGCGCT
ApoI 641 GAATTC [GA]^AATT[CT]
754 AAATTT
AvaII 470 GGACC G^G[AT]CC
1356 GGTCC
BciVI 223 GGATAC GTATCC(6/5)
448 GGATAC
BcnI 422 CCCGG CC^[GC]GG
597 CCGGG
BsgI 989 CTGCAC GTGCAG(16/14)
950 GTGCAG
BsrBI 466 GAGCGG CCGCTC(-3/-3)
501 GAGCGG
BsrFI 506 GCCGGC [GA]^CCGG[CT]
1064 ACCGGC
BsrI 309 CCAGT ACTGG(1/-1)
711 CCAGT
BstUI 24 CGCG CG^CG
70 CGCG
Cfr10I 506 GCCGGC [GA]^CCGG[CT]
1064 ACCGGC
DdeI 734 CTGAG C^T[ACGT]AG
1207 CTCAG
DraII 208 AGGGCCC [GA]G^G[ACGT]CC[CT]
209 GGGCCCC
DsaI 163 CCGTGG C^C[GA][CT]GG
1124 CCGTGG
EaeI 504 CGGCCG [CT]^GGCC[GA]
508 CGGCCA
Eco47III 1197 AGCGCT AGC^GCT
1280 AGCGCT
EcoO109I 208 AGGGCCC [GA]G^G[ACGT]CC[CT]
209 GGGCCCC
HaeII 1197 AGCGCT [GA]GCGC^[CT]
1280 AGCGCT
MaeII 454 ACGT A^CGT
962 ACGT
MvnI 24 CGCG CG^CG
70 CGCG
NciI 422 CCCGG CC^[GC]GG
597 CCGGG
PflMI 376 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
802 CCAGAGAATGG
RsaI 201 GTAC GT^AC
714 GTAC
SfaNI 1299 GATGC GCATC(5/9)
869 GCATC
SmlI 215 CTCAAG C^T[CT][GA]AG
1338 CTTGAG
StuI 494 AGGCCT AGG^CCT
666 AGGCCT
TaqI 759 TCGA T^CGA
1113 TCGA
TfiI 542 GAATC G^A[AT]TC
1223 GAATC
TthHB8I 759 TCGA T^CGA
1113 TCGA
Van91I 376 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
802 CCAGAGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AlwI AspHI BanII
BbvI BfaI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BssKI BstNI Cac8I CfoI Cfr13I
DpnII EarI EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PstI Sau3AI
Sau96I ScrFI SfcI Tru9I TseI
Tsp509I