Restriction map for
pF1KB9140
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BclI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsgI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FokI FseI HgaI HincII HindII
HindIII HpaI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AciI 11 CCGC CCGC(-3/-1)
AcsI 607 GAATTT [GA]^AATT[CT]
AcyI 17 GGCGCC G[GA]^CG[CT]C
AfaI 949 GTAC GT^AC
AflII 261 CTTAAG C^TTAAG
AlwNI 953 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 553 CCTGAGG CC^T[ACGT]AGG
ApoI 607 GAATTT [GA]^AATT[CT]
Asp700I 1049 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 128 CTCGAG C^[CT]CG[GA]G
AviII 473 TGCGCA TGC^GCA
BanI 17 GGCGCC G^G[CT][GA]CC
BbeI 17 GGCGCC GGCGC^C
BfrI 261 CTTAAG C^TTAAG
BglII 507 AGATCT A^GATCT
BsaHI 17 GGCGCC G[GA]^CG[CT]C
BsaI 152 GAGACC GGTCTC(1/5)
BsiEI 26 CGATCG CG[GA][CT]^CG
BsmFI 1081 GGGAC GGGAC(10/14)
BsoBI 128 CTCGAG C^[CT]CG[GA]G
Bsu36I 553 CCTGAGG CC^T[ACGT]AGG
Eco81I 553 CCTGAGG CC^T[ACGT]AGG
EcoT14I 71 CCATGG C^C[AT][AT]GG
FspI 473 TGCGCA TGC^GCA
HaeII 17 GGCGCC [GA]GCGC^[CT]
Hin1I 17 GGCGCC G[GA]^CG[CT]C
KasI 17 GGCGCC G^GCGCC
MspA1I 51 CAGCTG C[AC]G^C[GT]G
NarI 17 GGCGCC GG^CGCC
NcoI 71 CCATGG C^CATGG
NspI 883 GCATGT [GA]CATG^[CT]
PaeR7I 128 CTCGAG C^TCGAG
PvuI 26 CGATCG CGAT^CG
PvuII 51 CAGCTG CAG^CTG
RsaI 949 GTAC GT^AC
SapI 736 GAAGAGC GCTCTTC(1/4)
SfcI 306 CTATAG C^T[GA][CT]AG
SfoI 17 GGCGCC GGC^GCC
SspI 1063 AATATT AAT^ATT
StyI 71 CCATGG C^C[AT][AT]GG
Tsp509I 608 AATT ^AATT
XcmI 71 CCATGGAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 128 CTCGAG C^TCGAG
XmnI 1049 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwI 925 GATCC GGATC(4/5)
955 GATCC
AspI 91 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1031 GACTCAGTC
AvaII 658 GGACC G^G[AT]CC
1109 GGACC
BbsI 827 GAAGAC GAAGAC(2/6)
1053 GTCTTC
BbvI 40 GCAGC GCAGC(8/12)
791 GCAGC
BcnI 14 CCGGG CC^[GC]GG
44 CCGGG
BpuAI 827 GAAGAC GAAGAC(2/6)
1053 GTCTTC
BseRI 76 GAGGAG GAGGAG(10/8)
480 GAGGAG
BsiYI 37 CCAGCAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
550 CCACCTGAGGG
BslI 37 CCAGCAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
550 CCACCTGAGGG
BspHI 87 TCATGA T^CATGA
761 TCATGA
BstNI 189 CCAGG CC^[AT]GG
908 CCTGG
DsaI 71 CCATGG C^C[GA][CT]GG
241 CCGTGG
EcoRII 189 CCAGG ^CC[AT]GG
908 CCTGG
HaeIII 9 GGCC GG^CC
380 GGCC
HapII 14 CCGG C^CGG
44 CCGG
HpaII 14 CCGG C^CGG
44 CCGG
HphI 835 GGTGA GGTGA(8/7)
237 TCACC
MspI 14 CCGG C^CGG
44 CCGG
MvaI 189 CCAGG CC^[AT]GG
908 CCTGG
MwoI 34 GCTCCAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
782 GCAGTCTCAGC
NciI 14 CCGGG CC^[GC]GG
44 CCGGG
NlaIV 7 GGGGCC GG[ACGT]^[ACGT]CC
17 GGCGCC
PflFI 91 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1031 GACTCAGTC
RcaI 87 TCATGA T^CATGA
761 TCATGA
SfaNI 799 GATGC GCATC(5/9)
234 GCATC
SmlI 128 CTCGAG C^T[CT][GA]AG
261 CTTAAG
TseI 40 GCAGC G^C[AT]GC
791 GCAGC
Tth111I 91 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1031 GACTCAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AluI AspHI BanII BfaI BmyI
BpmI BsaJI BsiHKAI BsmAI Bsp1286I
BsrI BssKI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EarI Fnu4HI
HhaI HinP1I HinfI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI NdeII NlaIII PleI
SacI Sau3AI Sau96I ScrFI TaqI
TfiI Tru9I Tsp45I TspRI TthHB8I
XhoII