Restriction map for pF1KB9387
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AvaI        AvrII       
BanI        BanII       BbeI        BbvCI       BcgI        
BciVI       BfaI        BfrI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BsaBI       BseAI       
BsiHKAI     BsiWI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspLU11I    BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DdeI        DraI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FseI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        MaeI        MaeIII      MamI        MfeI        
MluI        MroI        MseI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PvuII       RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TfiI        
Tru9I       Tsp45I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AcyI          834  GGCGTC               G[GA]^CG[CT]C
AspI          405  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         335  TGCGCA               TGC^GCA
BamHI         440  GGATCC               G^GATCC
BbrPI          64  CACGTG               CAC^GTG
BclI          797  TGATCA               T^GATCA
BglI          175  GCCATCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI         834  GGCGTC               G[GA]^CG[CT]C
BsaI          613  GAGACC               GGTCTC(1/5)
BsaWI         437  ACCGGA               [AT]^CCGG[AT]
BsgI          631  CTGCAC               GTGCAG(16/14)
BsiEI         281  CGATCG               CG[GA][CT]^CG
BsmBI         583  GAGACG               CGTCTC(1/5)
BspHI         794  TCATGA               T^CATGA
BsrBI         507  GAGCGG               CCGCTC(-3/-3)
DsaI          326  CCGCGG               C^C[GA][CT]GG
FauI          509  GCGGG                CCCGC(4/6)
FbaI          797  TGATCA               T^GATCA
FspI          335  TGCGCA               TGC^GCA
Hin1I         834  GGCGTC               G[GA]^CG[CT]C
HphI          156  GGTGA                GGTGA(8/7)
KspI          326  CCGCGG               CCGC^GG
MwoI          175  GCCATCCTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI          497  ACATGC               [GA]CATG^[CT]
PflFI         405  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI          78  CCACTTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI          64  CACGTG               CAC^GTG
PmlI           64  CACGTG               CAC^GTG
PstI          233  CTGCAG               CTGCA^G
PvuI          281  CGATCG               CGAT^CG
RcaI          794  TCATGA               T^CATGA
SacII         326  CCGCGG               CCGC^GG
SfcI          233  CTGCAG               C^T[GA][CT]AG
SmlI           14  CTCAAG               C^T[CT][GA]AG
Tsp509I       272  AATT                 ^AATT
Tth111I       405  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I         78  CCACTTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI          417  GTAC                 GT^AC
              699  GTAC                
AlwNI         214  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              565  CAGGCGCTG           
BalI          518  TGGCCA               TGG^CCA
              653  TGGCCA              
BsaAI          64  CACGTG               [CT]AC^GT[GA]
              724  TACGTG              
BseRI          52  GAGGAG               GAGGAG(10/8)
              304  GAGGAG              
BsmAI         583  GAGAC                GTCTC(1/5)
              613  GAGAC               
BspMI         332  ACCTGC               ACCTGC(4/8)
              153  GCAGGT              
BstNI         180  CCTGG                CC^[AT]GG
              672  CCAGG               
EarI           49  GAAGAG               CTCTTC(1/4)
              109  GAAGAG              
EcoRII        180  CCTGG                ^CC[AT]GG
              672  CCAGG               
HaeII         172  AGCGCC               [GA]GCGC^[CT]
              567  GGCGCT              
HgaI          852  GACGC                GACGC(5/10)
              835  GCGTC               
HinfI          12  GACTC                G^A[ACGT]TC
              547  GACTC               
Ksp632I        49  GAAGAG               CTCTTC(1/4)
              109  GAAGAG              
MluNI         518  TGGCCA               TGG^CCA
              653  TGGCCA              
MscI          518  TGGCCA               TGG^CCA
              653  TGGCCA              
MslI          537  CATCAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              651  CATGGCCATG          
MvaI          180  CCTGG                CC^[AT]GG
              672  CCAGG               
PleI           12  GACTC                GAGTC(4/5)
              547  GACTC               
RsaI          417  GTAC                 GT^AC
              699  GTAC                
TaqI          284  TCGA                 T^CGA
              695  TCGA                
TthHB8I       284  TCGA                 T^CGA
              695  TCGA                
XcmI          209  CCAAACAGGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              479  CCAACGAAGAACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AvaII       
BbsI        BbvI        BcnI        BpmI        BpuAI       
BsaJI       BsiYI       BslI        BsmFI       BsrI        
BssKI       BstF5I      BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DpnII       EaeI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MaeII       MboI        MboII       
MflI        MnlI        MspA1I      MspI        MvnI        
NciI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       SfaNI       TseI        TspRI       
XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com