################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvrII BanI BanII BbeI BbvCI BcgI BciVI BfaI BfrI BglII BlnI BlpI BmyI Bpu1102I BsaBI BseAI BsiHKAI BsiWI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI HincII HindII HindIII HpaI KasI KpnI MaeI MaeIII MamI MfeI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcyI 834 GGCGTC G[GA]^CG[CT]C AspI 405 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 335 TGCGCA TGC^GCA BamHI 440 GGATCC G^GATCC BbrPI 64 CACGTG CAC^GTG BclI 797 TGATCA T^GATCA BglI 175 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 834 GGCGTC G[GA]^CG[CT]C BsaI 613 GAGACC GGTCTC(1/5) BsaWI 437 ACCGGA [AT]^CCGG[AT] BsgI 631 CTGCAC GTGCAG(16/14) BsiEI 281 CGATCG CG[GA][CT]^CG BsmBI 583 GAGACG CGTCTC(1/5) BspHI 794 TCATGA T^CATGA BsrBI 507 GAGCGG CCGCTC(-3/-3) DsaI 326 CCGCGG C^C[GA][CT]GG FauI 509 GCGGG CCCGC(4/6) FbaI 797 TGATCA T^GATCA FspI 335 TGCGCA TGC^GCA Hin1I 834 GGCGTC G[GA]^CG[CT]C HphI 156 GGTGA GGTGA(8/7) KspI 326 CCGCGG CCGC^GG MwoI 175 GCCATCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspI 497 ACATGC [GA]CATG^[CT] PflFI 405 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 78 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 64 CACGTG CAC^GTG PmlI 64 CACGTG CAC^GTG PstI 233 CTGCAG CTGCA^G PvuI 281 CGATCG CGAT^CG RcaI 794 TCATGA T^CATGA SacII 326 CCGCGG CCGC^GG SfcI 233 CTGCAG C^T[GA][CT]AG SmlI 14 CTCAAG C^T[CT][GA]AG Tsp509I 272 AATT ^AATT Tth111I 405 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 78 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 417 GTAC GT^AC 699 GTAC AlwNI 214 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 565 CAGGCGCTG BalI 518 TGGCCA TGG^CCA 653 TGGCCA BsaAI 64 CACGTG [CT]AC^GT[GA] 724 TACGTG BseRI 52 GAGGAG GAGGAG(10/8) 304 GAGGAG BsmAI 583 GAGAC GTCTC(1/5) 613 GAGAC BspMI 332 ACCTGC ACCTGC(4/8) 153 GCAGGT BstNI 180 CCTGG CC^[AT]GG 672 CCAGG EarI 49 GAAGAG CTCTTC(1/4) 109 GAAGAG EcoRII 180 CCTGG ^CC[AT]GG 672 CCAGG HaeII 172 AGCGCC [GA]GCGC^[CT] 567 GGCGCT HgaI 852 GACGC GACGC(5/10) 835 GCGTC HinfI 12 GACTC G^A[ACGT]TC 547 GACTC Ksp632I 49 GAAGAG CTCTTC(1/4) 109 GAAGAG MluNI 518 TGGCCA TGG^CCA 653 TGGCCA MscI 518 TGGCCA TGG^CCA 653 TGGCCA MslI 537 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 651 CATGGCCATG MvaI 180 CCTGG CC^[AT]GG 672 CCAGG PleI 12 GACTC GAGTC(4/5) 547 GACTC RsaI 417 GTAC GT^AC 699 GTAC TaqI 284 TCGA T^CGA 695 TCGA TthHB8I 284 TCGA T^CGA 695 TCGA XcmI 209 CCAAACAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 479 CCAACGAAGAACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AccII AciI AluI AlwI AvaII BbsI BbvI BcnI BpmI BpuAI BsaJI BsiYI BslI BsmFI BsrI BssKI BstF5I BstUI BstYI Cac8I CfoI Cfr13I DpnII EaeI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MboI MboII MflI MnlI MspA1I MspI MvnI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI TspRI XhoII