Restriction map for
pF1KE1013
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AlwNI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp718I AspI AvrII BalI BamHI
BanI BbeI BbrPI BbsI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI HaeII HincII HindII
HindIII HpaI HphI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MseI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 283 GTCTAC GT^[AC][GT]AC
AflIII 362 ACACGT A^C[GA][CT]GT
AhdI 416 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 349 GTGCAC G^TGCAC
AlwI 265 GATCC GGATC(4/5)
ApaLI 349 GTGCAC G^TGCAC
AspEI 416 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 187 CTCGGG C^[CT]CG[GA]G
AviII 25 TGCGCA TGC^GCA
BanII 646 GAGCTC G[GA]GC[CT]^C
BbvCI 612 GCTGAGG CCTCAGC(-5/-2)
BciVI 534 GGATAC GTATCC(6/5)
BsmAI 748 GAGAC GTCTC(1/5)
BsmBI 748 GAGACG CGTCTC(1/5)
BsmI 311 GCATTC GAATGC(1/-1)
BsoBI 187 CTCGGG C^[CT]CG[GA]G
BspHI 485 TCATGA T^CATGA
BsrBI 781 GAGCGG CCGCTC(-3/-3)
BsrFI 552 GCCGGC [GA]^CCGG[CT]
Cfr10I 552 GCCGGC [GA]^CCGG[CT]
DdeI 613 CTGAG C^T[ACGT]AG
Eam1105I 416 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 510 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 25 TGCGCA TGC^GCA
HinfI 271 GACTC G^A[ACGT]TC
MslI 486 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 385 CAGCGG C[AC]G^C[GT]G
NaeI 552 GCCGGC GCC^GGC
NgoMIV 552 GCCGGC G^CCGGC
NspI 824 ACATGC [GA]CATG^[CT]
PleI 271 GACTC GAGTC(4/5)
PstI 220 CTGCAG CTGCA^G
RcaI 485 TCATGA T^CATGA
SacI 646 GAGCTC GAGCT^C
SfcI 220 CTGCAG C^T[GA][CT]AG
TaqI 203 TCGA T^CGA
TthHB8I 203 TCGA T^CGA
XcmI 407 CCAAAAGGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 643 CGCG CG^CG
778 CGCG
AcyI 589 GACGCC G[GA]^CG[CT]C
721 GACGCC
AfaI 318 GTAC GT^AC
528 GTAC
Asp700I 195 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
360 GAACACGTTC
AvaII 136 GGTCC G^G[AT]CC
246 GGTCC
BfaI 79 CTAG C^TAG
559 CTAG
BsaHI 589 GACGCC G[GA]^CG[CT]C
721 GACGCC
BsgI 3 GTGCAG GTGCAG(16/14)
787 GTGCAG
BstF5I 669 CATCC GGATG(2/0)
420 GGATG
BstUI 643 CGCG CG^CG
778 CGCG
EaeI 582 CGGCCA [CT]^GGCC[GA]
713 CGGCCA
EarI 511 CTCTTC CTCTTC(1/4)
760 GAAGAG
FokI 669 CATCC GGATG(9/13)
420 GGATG
Hin1I 589 GACGCC G[GA]^CG[CT]C
721 GACGCC
Ksp632I 511 CTCTTC CTCTTC(1/4)
760 GAAGAG
MaeI 79 CTAG C^TAG
559 CTAG
MaeII 364 ACGT A^CGT
677 ACGT
MboII 760 GAAGA GAAGA(8/7)
512 TCTTC
MvnI 643 CGCG CG^CG
778 CGCG
PpuMI 135 GGGTCCC [GA]G^G[AT]CC[CT]
245 GGGTCCT
RsaI 318 GTAC GT^AC
528 GTAC
SfaNI 278 GCATC GCATC(5/9)
806 GCATC
TspRI 212 CAGTG CA[GC]TG[ACGT][ACGT]^
218 CACTG
XmnI 195 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
360 GAACACGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AspHI BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstNI Cac8I CfoI Cfr13I
DpnII DraII EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HpaII ItaI MaeIII MboI
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TseI Tsp45I