Restriction map for
pF1KB0496
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccII AccIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BbrPI BciVI BclI
BcnI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BseRI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BsrBI BssHII BstEII BstPI
BstUI CelII ClaI CpoI DraIII
Eam1105I Eco47III EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HpaI
KpnI KspI MamI MluI MroI
MvnI NaeI NciI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccI 2454 GTATAC GT^[AC][GT]AC
AcyI 2993 GGCGCC G[GA]^CG[CT]C
AocI 1315 CCTCAGG CC^T[ACGT]AGG
AspHI 2653 GAGCTC G[AT]GC[AT]^C
BalI 1067 TGGCCA TGG^CCA
BamHI 2070 GGATCC G^GATCC
BanI 2993 GGCGCC G^G[CT][GA]CC
BbeI 2993 GGCGCC GGCGC^C
BbsI 262 GAAGAC GAAGAC(2/6)
BbvCI 488 CCTCAGC CCTCAGC(-5/-2)
BcgI 1041 GCAAGTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 262 GAAGAC GAAGAC(2/6)
BsaAI 1721 TACGTG [CT]AC^GT[GA]
BsaHI 2993 GGCGCC G[GA]^CG[CT]C
BsgI 479 CTGCAC GTGCAG(16/14)
BsiEI 2251 CGGCCG CG[GA][CT]^CG
BsiHKAI 2653 GAGCTC G[AT]GC[AT]^C
BsmFI 2185 GTCCC GGGAC(10/14)
Bsp1407I 328 TGTACA T^GTACA
BsrFI 2253 GCCGGT [GA]^CCGG[CT]
BsrGI 328 TGTACA T^GTACA
BssSI 380 CACGAG CACGAG(-5/-1)
Bst1107I 2454 GTATAC GTA^TAC
BstBI 202 TTCGAA TT^CGAA
BstXI 521 CCATTGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2070 GGATCC [GA]^GATC[CT]
BstZ17I 2454 GTATAC GTA^TAC
Bsu36I 1315 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2253 GCCGGT [GA]^CCGG[CT]
DraII 105 GGGGCCT [GA]G^G[ACGT]CC[CT]
EagI 2251 CGGCCG C^GGCCG
EclXI 2251 CGGCCG C^GGCCG
Eco52I 2251 CGGCCG C^GGCCG
Eco81I 1315 CCTCAGG CC^T[ACGT]AGG
EcoO109I 105 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1365 CCATGG C^C[AT][AT]GG
HaeII 2993 GGCGCC [GA]GCGC^[CT]
HapII 2254 CCGG C^CGG
HgaI 7 GACGC GACGC(5/10)
Hin1I 2993 GGCGCC G[GA]^CG[CT]C
HincII 451 GTTGAC GT[CT]^[GA]AC
HindII 451 GTTGAC GT[CT]^[GA]AC
HindIII 2393 AAGCTT A^AGCTT
HpaII 2254 CCGG C^CGG
KasI 2993 GGCGCC G^GCGCC
MflI 2070 GGATCC [GA]^GATC[CT]
MluNI 1067 TGGCCA TGG^CCA
MscI 1067 TGGCCA TGG^CCA
MspI 2254 CCGG C^CGG
NarI 2993 GGCGCC GG^CGCC
NcoI 1365 CCATGG C^CATGG
NspV 202 TTCGAA TT^CGAA
PshAI 2955 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2653 GAGCTC GAGCT^C
ScaI 2091 AGTACT AGT^ACT
SexAI 1965 ACCAGGT A^CC[AT]GGT
SfoI 2993 GGCGCC GGC^GCC
SfuI 202 TTCGAA TT^CGAA
SspBI 328 TGTACA T^GTACA
SspI 555 AATATT AAT^ATT
StuI 1430 AGGCCT AGG^CCT
StyI 1365 CCATGG C^C[AT][AT]GG
Tsp45I 1007 GTGAC ^GT[GC]AC
XhoII 2070 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AclI 137 AACGTT AA^CGTT
2303 AACGTT
AlwNI 113 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
2135 CAGAAGCTG
AseI 652 ATTAAT AT^TAAT
1735 ATTAAT
AsnI 652 ATTAAT AT^TAAT
1735 ATTAAT
AvaII 1108 GGACC G^G[AT]CC
2788 GGACC
BpmI 110 CTCCAG CTGGAG(16/14)
2924 CTGGAG
BsaI 1076 GAGACC GGTCTC(1/5)
1447 GAGACC
BsmAI 1076 GAGAC GTCTC(1/5)
1447 GAGAC
BsmI 10 GCATTC GAATGC(1/-1)
2351 GCATTC
BspLU11I 1326 ACATGT A^CATGT
1748 ACATGT
BspMI 1057 GCAGGT ACCTGC(4/8)
2178 GCAGGT
BstAPI 343 GCACTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2476 GCACAGATTGC
DraI 2277 TTTAAA TTT^AAA
2884 TTTAAA
DrdI 2789 GACCACTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3014 GACAAATGTGTC
DsaI 1365 CCATGG C^C[GA][CT]GG
1442 CCACGG
EaeI 1067 TGGCCA [CT]^GGCC[GA]
2251 CGGCCG
EcoRI 1818 GAATTC G^AATTC
2748 GAATTC
MfeI 1280 CAATTG C^AATTG
1637 CAATTG
MslI 1363 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1551 CATTCAGATG
MspA1I 491 CAGCGG C[AC]G^C[GT]G
1664 CCGCTG
MunI 1280 CAATTG C^AATTG
1637 CAATTG
PflMI 379 CCACGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1633 CCAACAATTGG
PshBI 652 ATTAAT AT^TAAT
1735 ATTAAT
Psp1406I 137 AACGTT AA^CGTT
2303 AACGTT
Van91I 379 CCACGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1633 CCAACAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI ApoI Asp700I BanII
BbvI BfaI BfrI BmyI BsaJI
BsiYI BslI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XmnI