Restriction map for
pF1KB6171
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII BamHI BbrPI BcgI BfrI
BlpI Bpu1102I BsaAI BsgI BsiWI
BspDI BspHI BspLU11I BsrBI BssHII
Bst1107I BstAPI BstBI BstZ17I CelII
ClaI CpoI DraIII DrdI Eam1105I
Eco47III EcoNI EcoRI EcoRV FseI
FspI HincII HindII HindIII HpaI
KpnI MfeI MluI MunI NdeI
NheI NotI NruI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccII 142 CGCG CG^CG
AccIII 266 TCCGGA T^CCGGA
AcsI 810 GAATTT [GA]^AATT[CT]
AcyI 63 GGCGCC G[GA]^CG[CT]C
Alw44I 1113 GTGCAC G^TGCAC
AlwNI 1930 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 58 CCTCAGG CC^T[ACGT]AGG
ApaI 54 GGGCCC GGGCC^C
ApaLI 1113 GTGCAC G^TGCAC
ApoI 810 GAATTT [GA]^AATT[CT]
AspI 1077 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 660 CTCGGG C^[CT]CG[GA]G
AvaII 1458 GGACC G^G[AT]CC
AvrII 1842 CCTAGG C^CTAGG
BbeI 63 GGCGCC GGCGC^C
BbvCI 757 GCTGAGG CCTCAGC(-5/-2)
BciVI 1008 GTATCC GTATCC(6/5)
BglII 1175 AGATCT A^GATCT
BlnI 1842 CCTAGG C^CTAGG
BsaBI 1003 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 63 GGCGCC G[GA]^CG[CT]C
BseAI 266 TCCGGA T^CCGGA
BsiEI 144 CGGCCG CG[GA][CT]^CG
BsmBI 435 CGTCTC CGTCTC(1/5)
BsmI 1067 GAATGC GAATGC(1/-1)
BsoBI 660 CTCGGG C^[CT]CG[GA]G
Bsp1407I 929 TGTACA T^GTACA
BspEI 266 TCCGGA T^CCGGA
BsrFI 1466 GCCGGC [GA]^CCGG[CT]
BsrGI 929 TGTACA T^GTACA
BssSI 961 CTCGTG CACGAG(-5/-1)
BstUI 142 CGCG CG^CG
Bsu36I 58 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1466 GCCGGC [GA]^CCGG[CT]
DraI 1375 TTTAAA TTT^AAA
EagI 144 CGGCCG C^GGCCG
EclXI 144 CGGCCG C^GGCCG
Eco52I 144 CGGCCG C^GGCCG
Eco81I 58 CCTCAGG CC^T[ACGT]AGG
EcoT22I 835 ATGCAT ATGCA^T
HgaI 1634 GCGTC GACGC(5/10)
Hin1I 63 GGCGCC G[GA]^CG[CT]C
KasI 63 GGCGCC G^GCGCC
KspI 141 CCGCGG CCGC^GG
MaeII 1133 ACGT A^CGT
MamI 1003 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 266 TCCGGA T^CCGGA
MvnI 142 CGCG CG^CG
NaeI 1466 GCCGGC GCC^GGC
NarI 63 GGCGCC GG^CGCC
NgoMIV 1466 GCCGGC G^CCGGC
NsiI 835 ATGCAT ATGCA^T
PflFI 1077 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1600 CAGCTG CAG^CTG
SacI 1942 GAGCTC GAGCT^C
SacII 141 CCGCGG CCGC^GG
SapI 1333 GAAGAGC GCTCTTC(1/4)
SfiI 1597 GGCCAGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 63 GGCGCC GGC^GCC
SspBI 929 TGTACA T^GTACA
Tth111I 1077 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwI 1541 GATCC GGATC(4/5)
167 GGATC
BbsI 223 GTCTTC GAAGAC(2/6)
1654 GTCTTC
BclI 489 TGATCA T^GATCA
986 TGATCA
BglI 1598 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1760 GCCCACAGGGC
BpuAI 223 GTCTTC GAAGAC(2/6)
1654 GTCTTC
BsaI 358 GAGACC GGTCTC(1/5)
874 GAGACC
BsaWI 266 TCCGGA [AT]^CCGG[AT]
467 ACCGGA
BspMI 775 ACCTGC ACCTGC(4/8)
1829 ACCTGC
BsrDI 1680 CATTGC GCAATG(2/0)
1048 GCAATG
BstEII 771 GGTGACC G^GT[ACGT]ACC
1053 GGTCACC
BstPI 771 GGTGACC G^GT[ACGT]ACC
1053 GGTCACC
BstXI 593 CCAAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1394 CCACAAGCCTGG
BstYI 1175 AGATCT [GA]^GATC[CT]
1540 AGATCC
EarI 940 GAAGAG CTCTTC(1/4)
1333 GAAGAG
EcoO65I 771 GGTGACC G^GT[ACGT]ACC
1053 GGTCACC
FauI 140 CCCGC CCCGC(4/6)
1685 CCCGC
FbaI 489 TGATCA T^GATCA
986 TGATCA
HaeII 63 GGCGCC [GA]GCGC^[CT]
407 AGCGCC
Ksp632I 940 GAAGAG CTCTTC(1/4)
1333 GAAGAG
MflI 1175 AGATCT [GA]^GATC[CT]
1540 AGATCC
MseI 74 TTAA T^TAA
1376 TTAA
MslI 129 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
888 CACCTCCATG
NcoI 114 CCATGG C^CATGG
893 CCATGG
PflMI 1706 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1929 CCAGATGCTGG
Tru9I 74 TTAA T^TAA
1376 TTAA
Tsp509I 811 AATT ^AATT
844 AATT
Van91I 1706 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1929 CCAGATGCTGG
XcmI 593 CCAAGCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
828 CCACCCGATGCATGG
XhoII 1175 AGATCT [GA]^GATC[CT]
1540 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AspHI BalI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I