Restriction map for
pF1KB6183
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# NO CUT ENZYMES ( 101 enzymes) #
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Acc65I AccII AclI AcsI AflII
AflIII AgeI Alw44I ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BcgI BciVI
BclI BfrI BlnI BsaBI BsiEI
BsiWI Bsp1407I BspDI BspLU11I BsrDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstUI BstZ17I Cfr10I ClaI CpoI
DraI EagI EclXI Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MamI
MfeI MluI MseI MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 549 GACGTC GACGT^C
AccI 89 GTCGAC GT^[AC][GT]AC
AccIII 1922 TCCGGA T^CCGGA
AhdI 751 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1510 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1112 AGCGCT AGC^GCT
ApaI 58 GGGCCC GGGCC^C
AspEI 751 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1835 TGGCCA TGG^CCA
BamHI 250 GGATCC G^GATCC
BbeI 40 GGCGCC GGCGC^C
BfaI 643 CTAG C^TAG
BglII 1382 AGATCT A^GATCT
BseAI 1922 TCCGGA T^CCGGA
BsmI 1297 GAATGC GAATGC(1/-1)
BspEI 1922 TCCGGA T^CCGGA
BspHI 1610 TCATGA T^CATGA
BsrBI 1254 CCGCTC CCGCTC(-3/-3)
Bsu36I 1510 CCTGAGG CC^T[ACGT]AGG
DraIII 1126 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 67 GACCAGGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 751 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1112 AGCGCT AGC^GCT
Eco81I 1510 CCTGAGG CC^T[ACGT]AGG
EcoNI 1305 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 89 GTCGAC GT[CT]^[GA]AC
HindII 89 GTCGAC GT[CT]^[GA]AC
KasI 40 GGCGCC G^GCGCC
MaeI 643 CTAG C^TAG
MluNI 1835 TGGCCA TGG^CCA
MroI 1922 TCCGGA T^CCGGA
MscI 1835 TGGCCA TGG^CCA
NarI 40 GGCGCC GG^CGCC
PpuMI 790 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1610 TCATGA T^CATGA
SalI 89 GTCGAC G^TCGAC
SfoI 40 GGCGCC GGC^GCC
StuI 1850 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 27 enzymes) #
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BbrPI 1222 CACGTG CAC^GTG
1324 CACGTG
BbvCI 1777 CCTCAGC CCTCAGC(-5/-2)
312 GCTGAGG
BlpI 829 GCTGAGC GC^T[ACGT]AGC
1256 GCTCAGC
Bpu1102I 829 GCTGAGC GC^T[ACGT]AGC
1256 GCTCAGC
BsaI 1663 GAGACC GGTCTC(1/5)
1446 GGTCTC
BsaWI 1841 ACCGGA [AT]^CCGG[AT]
1922 TCCGGA
BssSI 398 CACGAG CACGAG(-5/-1)
1696 CACGAG
BstAPI 285 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1311 GCAGGCCCTGC
BstEII 1062 GGTCACC G^GT[ACGT]ACC
1809 GGTTACC
BstPI 1062 GGTCACC G^GT[ACGT]ACC
1809 GGTTACC
BstXI 47 CCACGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1265 CCACAGTGCTGG
CelII 829 GCTGAGC GC^T[ACGT]AGC
1256 GCTCAGC
EcoO65I 1062 GGTCACC G^GT[ACGT]ACC
1809 GGTTACC
EcoT14I 1640 CCTTGG C^C[AT][AT]GG
1748 CCAAGG
HgaI 1182 GACGC GACGC(5/10)
1247 GCGTC
NspI 628 GCATGT [GA]CATG^[CT]
1673 ACATGC
PmaCI 1222 CACGTG CAC^GTG
1324 CACGTG
PmlI 1222 CACGTG CAC^GTG
1324 CACGTG
SacI 217 GAGCTC GAGCT^C
1333 GAGCTC
SapI 1570 GAAGAGC GCTCTTC(1/4)
1711 GAAGAGC
SmaI 30 CCCGGG CCC^GGG
1085 CCCGGG
StyI 1640 CCTTGG C^C[AT][AT]GG
1748 CCAAGG
TaqI 90 TCGA T^CGA
944 TCGA
TthHB8I 90 TCGA T^CGA
944 TCGA
XcmI 470 CCAGCTGCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1619 CCATCAGTCTTCTGG
XmaCI 30 CCCGGG C^CCGGG
1085 CCCGGG
XmaI 30 CCCGGG C^CCGGG
1085 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TseI Tsp45I TspRI
Van91I XhoII